Extrachromosomal DNA of pea (Pisum sativum) root-tip cells replicates by strand displacement
In cultured pea roots there is extrachromosomal DNA associated with cells that differentiate from the G2phase of the cell cycle that is absent from those that differentiate from the G1phase. We examined this extrachromosomal DNA by electron microscopy and found that it consisted of three types: (i)...
Gespeichert in:
| Veröffentlicht in: | Proc. Natl. Acad. Sci. U.S.A.; (United States) 1983-04, Vol.80 (7), p.1933-1937 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1937 |
|---|---|
| container_issue | 7 |
| container_start_page | 1933 |
| container_title | Proc. Natl. Acad. Sci. U.S.A.; (United States) |
| container_volume | 80 |
| creator | Krimer, D. B. J. Van't Hof |
| description | In cultured pea roots there is extrachromosomal DNA associated with cells that differentiate from the G2phase of the cell cycle that is absent from those that differentiate from the G1phase. We examined this extrachromosomal DNA by electron microscopy and found that it consisted of three types: (i) double-stranded linear molecules with single-stranded branches (74%), (ii) double-stranded molecules without branches (26%), and (iii) free single-stranded molecules. The double-stranded molecules with or without branches were similar in length, having a modal length of 10-15 μ m. The free single-stranded molecules were shorter and had a mean length of 3.8 μ m. The length of the branches attached to the duplex molecules was only slightly less than that of the free form. The duplex molecules with branches were interpreted as configurations reflecting an ongoing strand-displacement process that results in free single-stranded molecules. Finally, measurements on duplex molecules with multiple branches suggested that the extrachromosomal DNA may exist in the form of tandemly repeated sequences. |
| doi_str_mv | 10.1073/pnas.80.7.1933 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_osti_</sourceid><recordid>TN_cdi_proquest_miscellaneous_733505290</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>13408</jstor_id><sourcerecordid>13408</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-6e49ac55fc99572c2fbc9186a79d47c5b2df3419dc16b7e56b1ecd7c3705769e3</originalsourceid><addsrcrecordid>eNp9ks9v1iAcxonRuNfp1YOJhniYemiFAgUOHpZt_kgWNdHdTAildC9LWyrQZfvvpXurm5edOHw_z_d54AGA5xiVGHHyfhp1LAUqeYklIQ_ABiOJi5pK9BBsEKp4IWhF98CTGC8QQpIJ9Bjs4ZplGlUb8OvkKgVttsEPPvpB9_D46yH0HZyshm-_uzgPMOrkLufhHQzepyK5CRrb9xEGO_XO6GQjbK5hzHvGFrYuTr02drBjegoedbqP9tl67oOzjyc_jz4Xp98-fTk6PC0M5TgVtaVSG8Y6IyXjlam6xkgsas1lS7lhTdV2hGLZGlw33LK6wda03BCOGK-lJfvgw27vNDeDbU22DrpXU3CDDtfKa6f-n4xuq879pSKS8Ipl_eud3sfkVDQuWbM1fhytSaomQkqOMvRmNQn-92xjUoOLy0Po0fo5Kk4IQ6ySC3lwL4kJ41gikcFyB5rgYwy2-xcZI7XUq5Z6lUCKq6XeLHh196K3-NpnBl6uwCL8O7674OC-uermvk_2KmXwxQ68iMmHWyNCb3KvMTrtlT4PLqqzH1gKmj9cJQQlfwDTwcuZ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13571908</pqid></control><display><type>article</type><title>Extrachromosomal DNA of pea (Pisum sativum) root-tip cells replicates by strand displacement</title><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Krimer, D. B. ; J. Van't Hof</creator><creatorcontrib>Krimer, D. B. ; J. Van't Hof ; Eidg. Forschungsanstalt Waedenswil (Switzerland) ; Brookhaven National Lab., Upton, NY</creatorcontrib><description>In cultured pea roots there is extrachromosomal DNA associated with cells that differentiate from the G2phase of the cell cycle that is absent from those that differentiate from the G1phase. We examined this extrachromosomal DNA by electron microscopy and found that it consisted of three types: (i) double-stranded linear molecules with single-stranded branches (74%), (ii) double-stranded molecules without branches (26%), and (iii) free single-stranded molecules. The double-stranded molecules with or without branches were similar in length, having a modal length of 10-15 μ m. The free single-stranded molecules were shorter and had a mean length of 3.8 μ m. The length of the branches attached to the duplex molecules was only slightly less than that of the free form. The duplex molecules with branches were interpreted as configurations reflecting an ongoing strand-displacement process that results in free single-stranded molecules. Finally, measurements on duplex molecules with multiple branches suggested that the extrachromosomal DNA may exist in the form of tandemly repeated sequences.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.80.7.1933</identifier><identifier>PMID: 16593302</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>550300 - Cytology ; BACTERIA ; BASIC BIOLOGICAL SCIENCES ; Biological Sciences: Cell Biology ; Cell biology ; CELL DIFFERENTIATION ; Cells ; Circles ; DATA ; DNA ; Electron micrographs ; ELECTRON MICROSCOPY ; EXPERIMENTAL DATA ; Formamides ; INFORMATION ; Interphase ; LEGUMINOSAE ; MICROORGANISMS ; MICROSCOPY ; MOLECULAR BIOLOGY ; MOLECULAR STRUCTURE ; MOLECULES ; NUCLEIC ACIDS ; NUMERICAL DATA ; ORGANIC COMPOUNDS ; Peas ; PISUM ; Pisum sativum ; PLANT CELLS ; PLANTS ; RHIZOBIUM ; ROOTS</subject><ispartof>Proc. Natl. Acad. Sci. U.S.A.; (United States), 1983-04, Vol.80 (7), p.1933-1937</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-6e49ac55fc99572c2fbc9186a79d47c5b2df3419dc16b7e56b1ecd7c3705769e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/80/7.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/13408$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/13408$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16593302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6389970$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Krimer, D. B.</creatorcontrib><creatorcontrib>J. Van't Hof</creatorcontrib><creatorcontrib>Eidg. Forschungsanstalt Waedenswil (Switzerland)</creatorcontrib><creatorcontrib>Brookhaven National Lab., Upton, NY</creatorcontrib><title>Extrachromosomal DNA of pea (Pisum sativum) root-tip cells replicates by strand displacement</title><title>Proc. Natl. Acad. Sci. U.S.A.; (United States)</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>In cultured pea roots there is extrachromosomal DNA associated with cells that differentiate from the G2phase of the cell cycle that is absent from those that differentiate from the G1phase. We examined this extrachromosomal DNA by electron microscopy and found that it consisted of three types: (i) double-stranded linear molecules with single-stranded branches (74%), (ii) double-stranded molecules without branches (26%), and (iii) free single-stranded molecules. The double-stranded molecules with or without branches were similar in length, having a modal length of 10-15 μ m. The free single-stranded molecules were shorter and had a mean length of 3.8 μ m. The length of the branches attached to the duplex molecules was only slightly less than that of the free form. The duplex molecules with branches were interpreted as configurations reflecting an ongoing strand-displacement process that results in free single-stranded molecules. Finally, measurements on duplex molecules with multiple branches suggested that the extrachromosomal DNA may exist in the form of tandemly repeated sequences.</description><subject>550300 - Cytology</subject><subject>BACTERIA</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological Sciences: Cell Biology</subject><subject>Cell biology</subject><subject>CELL DIFFERENTIATION</subject><subject>Cells</subject><subject>Circles</subject><subject>DATA</subject><subject>DNA</subject><subject>Electron micrographs</subject><subject>ELECTRON MICROSCOPY</subject><subject>EXPERIMENTAL DATA</subject><subject>Formamides</subject><subject>INFORMATION</subject><subject>Interphase</subject><subject>LEGUMINOSAE</subject><subject>MICROORGANISMS</subject><subject>MICROSCOPY</subject><subject>MOLECULAR BIOLOGY</subject><subject>MOLECULAR STRUCTURE</subject><subject>MOLECULES</subject><subject>NUCLEIC ACIDS</subject><subject>NUMERICAL DATA</subject><subject>ORGANIC COMPOUNDS</subject><subject>Peas</subject><subject>PISUM</subject><subject>Pisum sativum</subject><subject>PLANT CELLS</subject><subject>PLANTS</subject><subject>RHIZOBIUM</subject><subject>ROOTS</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><recordid>eNp9ks9v1iAcxonRuNfp1YOJhniYemiFAgUOHpZt_kgWNdHdTAildC9LWyrQZfvvpXurm5edOHw_z_d54AGA5xiVGHHyfhp1LAUqeYklIQ_ABiOJi5pK9BBsEKp4IWhF98CTGC8QQpIJ9Bjs4ZplGlUb8OvkKgVttsEPPvpB9_D46yH0HZyshm-_uzgPMOrkLufhHQzepyK5CRrb9xEGO_XO6GQjbK5hzHvGFrYuTr02drBjegoedbqP9tl67oOzjyc_jz4Xp98-fTk6PC0M5TgVtaVSG8Y6IyXjlam6xkgsas1lS7lhTdV2hGLZGlw33LK6wda03BCOGK-lJfvgw27vNDeDbU22DrpXU3CDDtfKa6f-n4xuq879pSKS8Ipl_eud3sfkVDQuWbM1fhytSaomQkqOMvRmNQn-92xjUoOLy0Po0fo5Kk4IQ6ySC3lwL4kJ41gikcFyB5rgYwy2-xcZI7XUq5Z6lUCKq6XeLHh196K3-NpnBl6uwCL8O7674OC-uermvk_2KmXwxQ68iMmHWyNCb3KvMTrtlT4PLqqzH1gKmj9cJQQlfwDTwcuZ</recordid><startdate>19830401</startdate><enddate>19830401</enddate><creator>Krimer, D. B.</creator><creator>J. Van't Hof</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19830401</creationdate><title>Extrachromosomal DNA of pea (Pisum sativum) root-tip cells replicates by strand displacement</title><author>Krimer, D. B. ; J. Van't Hof</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-6e49ac55fc99572c2fbc9186a79d47c5b2df3419dc16b7e56b1ecd7c3705769e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>550300 - Cytology</topic><topic>BACTERIA</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological Sciences: Cell Biology</topic><topic>Cell biology</topic><topic>CELL DIFFERENTIATION</topic><topic>Cells</topic><topic>Circles</topic><topic>DATA</topic><topic>DNA</topic><topic>Electron micrographs</topic><topic>ELECTRON MICROSCOPY</topic><topic>EXPERIMENTAL DATA</topic><topic>Formamides</topic><topic>INFORMATION</topic><topic>Interphase</topic><topic>LEGUMINOSAE</topic><topic>MICROORGANISMS</topic><topic>MICROSCOPY</topic><topic>MOLECULAR BIOLOGY</topic><topic>MOLECULAR STRUCTURE</topic><topic>MOLECULES</topic><topic>NUCLEIC ACIDS</topic><topic>NUMERICAL DATA</topic><topic>ORGANIC COMPOUNDS</topic><topic>Peas</topic><topic>PISUM</topic><topic>Pisum sativum</topic><topic>PLANT CELLS</topic><topic>PLANTS</topic><topic>RHIZOBIUM</topic><topic>ROOTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krimer, D. B.</creatorcontrib><creatorcontrib>J. Van't Hof</creatorcontrib><creatorcontrib>Eidg. Forschungsanstalt Waedenswil (Switzerland)</creatorcontrib><creatorcontrib>Brookhaven National Lab., Upton, NY</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krimer, D. B.</au><au>J. Van't Hof</au><aucorp>Eidg. Forschungsanstalt Waedenswil (Switzerland)</aucorp><aucorp>Brookhaven National Lab., Upton, NY</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extrachromosomal DNA of pea (Pisum sativum) root-tip cells replicates by strand displacement</atitle><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1983-04-01</date><risdate>1983</risdate><volume>80</volume><issue>7</issue><spage>1933</spage><epage>1937</epage><pages>1933-1937</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>In cultured pea roots there is extrachromosomal DNA associated with cells that differentiate from the G2phase of the cell cycle that is absent from those that differentiate from the G1phase. We examined this extrachromosomal DNA by electron microscopy and found that it consisted of three types: (i) double-stranded linear molecules with single-stranded branches (74%), (ii) double-stranded molecules without branches (26%), and (iii) free single-stranded molecules. The double-stranded molecules with or without branches were similar in length, having a modal length of 10-15 μ m. The free single-stranded molecules were shorter and had a mean length of 3.8 μ m. The length of the branches attached to the duplex molecules was only slightly less than that of the free form. The duplex molecules with branches were interpreted as configurations reflecting an ongoing strand-displacement process that results in free single-stranded molecules. Finally, measurements on duplex molecules with multiple branches suggested that the extrachromosomal DNA may exist in the form of tandemly repeated sequences.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>16593302</pmid><doi>10.1073/pnas.80.7.1933</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proc. Natl. Acad. Sci. U.S.A.; (United States), 1983-04, Vol.80 (7), p.1933-1937 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733505290 |
| source | JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 550300 - Cytology BACTERIA BASIC BIOLOGICAL SCIENCES Biological Sciences: Cell Biology Cell biology CELL DIFFERENTIATION Cells Circles DATA DNA Electron micrographs ELECTRON MICROSCOPY EXPERIMENTAL DATA Formamides INFORMATION Interphase LEGUMINOSAE MICROORGANISMS MICROSCOPY MOLECULAR BIOLOGY MOLECULAR STRUCTURE MOLECULES NUCLEIC ACIDS NUMERICAL DATA ORGANIC COMPOUNDS Peas PISUM Pisum sativum PLANT CELLS PLANTS RHIZOBIUM ROOTS |
| title | Extrachromosomal DNA of pea (Pisum sativum) root-tip cells replicates by strand displacement |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T15%3A45%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extrachromosomal%20DNA%20of%20pea%20(Pisum%20sativum)%20root-tip%20cells%20replicates%20by%20strand%20displacement&rft.jtitle=Proc.%20Natl.%20Acad.%20Sci.%20U.S.A.;%20(United%20States)&rft.au=Krimer,%20D.%20B.&rft.aucorp=Eidg.%20Forschungsanstalt%20Waedenswil%20(Switzerland)&rft.date=1983-04-01&rft.volume=80&rft.issue=7&rft.spage=1933&rft.epage=1937&rft.pages=1933-1937&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.80.7.1933&rft_dat=%3Cjstor_osti_%3E13408%3C/jstor_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13571908&rft_id=info:pmid/16593302&rft_jstor_id=13408&rfr_iscdi=true |