Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis
The various Leishmania species are flagellated protozoans, responsible for a wide spectrum of human diseases. The sequence of the L. major genome is nearing completion and a large proportion of the identified genes have yet to be ascribed functions. DNA microarrays containing PCR-amplified DNA from...
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| Veröffentlicht in: | Molecular and biochemical parasitology 2003-06, Vol.129 (1), p.103-114 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Saxena, Alka Worthey, Elizabeth A. Yan, Shaofeng Leland, Aaron Stuart, Kenneth D. Myler, Peter J. |
| description | The various
Leishmania species are flagellated protozoans, responsible for a wide spectrum of human diseases. The sequence of the
L. major genome is nearing completion and a large proportion of the identified genes have yet to be ascribed functions. DNA microarrays containing PCR-amplified DNA from a random amplified genomic library of
L. major Friedlin (LmjF) [Mol. Biochem. Parasitol. 113 (2001) 337] were hybridized with fluorescent probes made from
L. major Friedlin RNA from five time-points during differentiation from procyclics to metacyclics. The data were normalized for background and probe intensity and the relative abundance of RNA for each spot was calculated. Almost 15% (1387/9282) of the DNAs showed statistically significant (
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| doi_str_mv | 10.1016/S0166-6851(03)00100-2 |
| format | Article |
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Leishmania species are flagellated protozoans, responsible for a wide spectrum of human diseases. The sequence of the
L. major genome is nearing completion and a large proportion of the identified genes have yet to be ascribed functions. DNA microarrays containing PCR-amplified DNA from a random amplified genomic library of
L. major Friedlin (LmjF) [Mol. Biochem. Parasitol. 113 (2001) 337] were hybridized with fluorescent probes made from
L. major Friedlin RNA from five time-points during differentiation from procyclics to metacyclics. The data were normalized for background and probe intensity and the relative abundance of RNA for each spot was calculated. Almost 15% (1387/9282) of the DNAs showed statistically significant (
P<0.01) changes in expression (1.1–5-fold) during the transition, with 1.16% (108) showing up-regulation at two or more time-points and 0.14% (13) showing down-regulation. Northern blot analyses of selected genes confirmed these results. These studies confirmed the stage-specific expression of several known genes, as well as identifying a number of novel genes that are up-regulated in either procyclics or metacyclics.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/S0166-6851(03)00100-2</identifier><identifier>PMID: 12798511</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cloning, Molecular ; DNA microarray ; Fluorescence ; Gene expression ; Gene Expression Profiling ; Leishmania major ; Leishmania major - genetics ; Leishmania major - growth & development ; Leishmania major - metabolism ; Life Cycle Stages ; Metacyclic ; Oligonucleotide Array Sequence Analysis ; Procyclic ; RNA, Messenger - analysis ; RNA, Messenger - metabolism ; Up-Regulation</subject><ispartof>Molecular and biochemical parasitology, 2003-06, Vol.129 (1), p.103-114</ispartof><rights>2003 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-babe26eef7d5576f99a253565c0ec8eb0615df98b5874dc5c1616f61db4b90cf3</citedby><cites>FETCH-LOGICAL-c458t-babe26eef7d5576f99a253565c0ec8eb0615df98b5874dc5c1616f61db4b90cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166685103001002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12798511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saxena, Alka</creatorcontrib><creatorcontrib>Worthey, Elizabeth A.</creatorcontrib><creatorcontrib>Yan, Shaofeng</creatorcontrib><creatorcontrib>Leland, Aaron</creatorcontrib><creatorcontrib>Stuart, Kenneth D.</creatorcontrib><creatorcontrib>Myler, Peter J.</creatorcontrib><title>Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>The various
Leishmania species are flagellated protozoans, responsible for a wide spectrum of human diseases. The sequence of the
L. major genome is nearing completion and a large proportion of the identified genes have yet to be ascribed functions. DNA microarrays containing PCR-amplified DNA from a random amplified genomic library of
L. major Friedlin (LmjF) [Mol. Biochem. Parasitol. 113 (2001) 337] were hybridized with fluorescent probes made from
L. major Friedlin RNA from five time-points during differentiation from procyclics to metacyclics. The data were normalized for background and probe intensity and the relative abundance of RNA for each spot was calculated. Almost 15% (1387/9282) of the DNAs showed statistically significant (
P<0.01) changes in expression (1.1–5-fold) during the transition, with 1.16% (108) showing up-regulation at two or more time-points and 0.14% (13) showing down-regulation. Northern blot analyses of selected genes confirmed these results. These studies confirmed the stage-specific expression of several known genes, as well as identifying a number of novel genes that are up-regulated in either procyclics or metacyclics.</description><subject>Animals</subject><subject>Cloning, Molecular</subject><subject>DNA microarray</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Leishmania major</subject><subject>Leishmania major - genetics</subject><subject>Leishmania major - growth & development</subject><subject>Leishmania major - metabolism</subject><subject>Life Cycle Stages</subject><subject>Metacyclic</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Procyclic</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>Up-Regulation</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v3CAQQFHVqtkk_QmtOFXpwS3gBeNTFOWrlVbtIckZYRjSiWy8ATvq3vrTy2a37TEXEMybGYZHyHvOPnPG1ZebsqhKaclPWP2JMc5YJV6RBdeNqNql0K_J4h9yQA5zfmCMyUapt-SAi6Yt13xBfl8-2X62E46RjoF6DAESxAltT-8hAoVf6wQ5b-MY6Qow_xxsREsH-zAmepUQfF8i6zS6jevRZWqjpwNM9u95zhjv6cX3MzqgS6NNyW4KZPtNxnxM3gTbZ3i334_I3dXl7fnXavXj-tv52apyS6mnqrMdCAUQGi_LEKFtrZC1VNIxcBo6prj0odWd1M3SO-m44ioo7rtl1zIX6iPycVe3PPRxhjyZAbODvrcRxjmbpq4lqwV7EeRat0I2TQHlDiwz5ZwgmHXCwaaN4cxsHZlnR2YrwLDaPDsyouR92DeYuwH8_6y9lAKc7gAo__GEkEx2CNGBxwRuMn7EF1r8AQOvo8E</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>Saxena, Alka</creator><creator>Worthey, Elizabeth A.</creator><creator>Yan, Shaofeng</creator><creator>Leland, Aaron</creator><creator>Stuart, Kenneth D.</creator><creator>Myler, Peter J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis</title><author>Saxena, Alka ; Worthey, Elizabeth A. ; Yan, Shaofeng ; Leland, Aaron ; Stuart, Kenneth D. ; Myler, Peter J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-babe26eef7d5576f99a253565c0ec8eb0615df98b5874dc5c1616f61db4b90cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Cloning, Molecular</topic><topic>DNA microarray</topic><topic>Fluorescence</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Leishmania major</topic><topic>Leishmania major - genetics</topic><topic>Leishmania major - growth & development</topic><topic>Leishmania major - metabolism</topic><topic>Life Cycle Stages</topic><topic>Metacyclic</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Procyclic</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saxena, Alka</creatorcontrib><creatorcontrib>Worthey, Elizabeth A.</creatorcontrib><creatorcontrib>Yan, Shaofeng</creatorcontrib><creatorcontrib>Leland, Aaron</creatorcontrib><creatorcontrib>Stuart, Kenneth D.</creatorcontrib><creatorcontrib>Myler, Peter J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saxena, Alka</au><au>Worthey, Elizabeth A.</au><au>Yan, Shaofeng</au><au>Leland, Aaron</au><au>Stuart, Kenneth D.</au><au>Myler, Peter J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>129</volume><issue>1</issue><spage>103</spage><epage>114</epage><pages>103-114</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><abstract>The various
Leishmania species are flagellated protozoans, responsible for a wide spectrum of human diseases. The sequence of the
L. major genome is nearing completion and a large proportion of the identified genes have yet to be ascribed functions. DNA microarrays containing PCR-amplified DNA from a random amplified genomic library of
L. major Friedlin (LmjF) [Mol. Biochem. Parasitol. 113 (2001) 337] were hybridized with fluorescent probes made from
L. major Friedlin RNA from five time-points during differentiation from procyclics to metacyclics. The data were normalized for background and probe intensity and the relative abundance of RNA for each spot was calculated. Almost 15% (1387/9282) of the DNAs showed statistically significant (
P<0.01) changes in expression (1.1–5-fold) during the transition, with 1.16% (108) showing up-regulation at two or more time-points and 0.14% (13) showing down-regulation. Northern blot analyses of selected genes confirmed these results. These studies confirmed the stage-specific expression of several known genes, as well as identifying a number of novel genes that are up-regulated in either procyclics or metacyclics.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>12798511</pmid><doi>10.1016/S0166-6851(03)00100-2</doi><tpages>12</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cloning, Molecular DNA microarray Fluorescence Gene expression Gene Expression Profiling Leishmania major Leishmania major - genetics Leishmania major - growth & development Leishmania major - metabolism Life Cycle Stages Metacyclic Oligonucleotide Array Sequence Analysis Procyclic RNA, Messenger - analysis RNA, Messenger - metabolism Up-Regulation |
| title | Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis |
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