Independent Photocycles of the Spectrally Distinct Forms of Bacteriorhodopsin

Time-resolved, flash-induced difference absorbance spectra (300-700 nm) at pH 10.5 and 5 degrees C for the bacteriorhodopsin photocycle fast and slow decaying forms of the M intermediate (Mf and Ms, respectively) and R intermediate are reported. The main distinguishing features are as follows: For M...

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Veröffentlicht in:	Proc. Natl. Acad. Sci. USA; (United States) 1988-09, Vol.85 (17), p.6358-6361
Hauptverfasser:	Zs. Dancsházy, Govindjee, R., Ebrey, T. G.
Format:	Artikel
Sprache:	eng
Schlagworte:	550200 - Biochemistry Absorption spectra ABSORPTION SPECTROSCOPY bacteriorhodopsin Bacteriorhodopsins BASIC BIOLOGICAL SCIENCES BIOCHEMICAL REACTION KINETICS Biological and medical sciences Biological Sciences: Biophysics Biophysics Bleaching Flash spectra Fundamental and applied biological sciences. Psychology Halobacterium salinarium KINETICS MEMBRANES Molecular biophysics ORGANIC COMPOUNDS PH VALUE Photochemistry. Photosynthesis. Bioluminescence PHOTOSYNTHETIC MEMBRANES PIGMENTS plasma membranes PROTEINS Radiation-biomolecule interaction REACTION KINETICS RHODOPSIN Sampling rates Spectral index Spectrophotometers SPECTROSCOPY TEMPERATURE DEPENDENCE Wavelengths
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