Biotransformation of acrylonitrile to acrylamide using immobilized whole cells of Brevibacterium CH1 in a recycle fed-batch reactor
Acrylamide was produced from acrylonitrile using immobilized Brevibacterium CH1 cells that were isolated from soil and found to possess nitrile hydratase activity. The reaction conditions and stability of the enzyme activity were studied. The conversion yield was nearly 100%, including a trace amoun...
Gespeichert in:
| Veröffentlicht in: | Biotechnology and bioengineering 1989-07, Vol.34 (3), p.380-386 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 386 |
|---|---|
| container_issue | 3 |
| container_start_page | 380 |
| container_title | Biotechnology and bioengineering |
| container_volume | 34 |
| creator | Hwang, Jun Sik Chang, Ho Nam |
| description | Acrylamide was produced from acrylonitrile using immobilized Brevibacterium CH1 cells that were isolated from soil and found to possess nitrile hydratase activity. The reaction conditions and stability of the enzyme activity were studied. The conversion yield was nearly 100%, including a trace amount of acrylic acid. This strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of amidase toward acrylamide. A packed bed reactor was operated in a fed‐batch manner for acrylamide production of high concentration. The acrylonitrile concentration was maintained below 3% and the operating temperature at 4°C to minimize enzyme deactivation. |
| doi_str_mv | 10.1002/bit.260340311 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733498157</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733498157</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4411-49556f00fc7efcf8e817b82a44fdf35b612d4897a6b73701aa6323d5b118e3b13</originalsourceid><addsrcrecordid>eNp9kE2LFDEQhoMo7rh69Co5CJ56TXU---gMuruwKMjKgpeQpBM32t1Zk27X8eofN8MMoydPRRVPVb08CD0HcgaEtK9tnM9aQSgjFOABWgHpZEPajjxEK0KIaCjv2hP0pJSvtZVKiMfoBBRXCoCt0O91THM2Uwkpj2aOacIpYOPydkhTnHMcPJ7TfmDG2Hu8lDh9wXEck41D_OV7fH-bKuX8MJTd8jr7H9EaN_sclxFvLgDHCRucvdu6CgbfN9bM7rZOKpXyU_QomKH4Z4d6ij69e3u9uWiuPpxfbt5cNY4xgIZ1nItASHDSBxeUVyCtag1joQ-UWwFtz1QnjbCSSgLGCNrSnlsA5akFeope7e_e5fR98WXWYyy72GbyaSlaUso6BVxWstmTLqdSsg_6LsfR5K0GonfaddWuj9or_-JwebGj7__SB88VeHkATHFmCNW4i-XICS5Zy3nF5B67r963_3-q15fX_yY4JI5l9j-PmyZ_06La4Prm_bn-yMjndtPdaEX_ABxdq0U</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733498157</pqid></control><display><type>article</type><title>Biotransformation of acrylonitrile to acrylamide using immobilized whole cells of Brevibacterium CH1 in a recycle fed-batch reactor</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Hwang, Jun Sik ; Chang, Ho Nam</creator><creatorcontrib>Hwang, Jun Sik ; Chang, Ho Nam</creatorcontrib><description>Acrylamide was produced from acrylonitrile using immobilized Brevibacterium CH1 cells that were isolated from soil and found to possess nitrile hydratase activity. The reaction conditions and stability of the enzyme activity were studied. The conversion yield was nearly 100%, including a trace amount of acrylic acid. This strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of amidase toward acrylamide. A packed bed reactor was operated in a fed‐batch manner for acrylamide production of high concentration. The acrylonitrile concentration was maintained below 3% and the operating temperature at 4°C to minimize enzyme deactivation.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260340311</identifier><identifier>PMID: 18588114</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies</subject><ispartof>Biotechnology and bioengineering, 1989-07, Vol.34 (3), p.380-386</ispartof><rights>Copyright © 1989 John Wiley & Sons, Inc.</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4411-49556f00fc7efcf8e817b82a44fdf35b612d4897a6b73701aa6323d5b118e3b13</citedby><cites>FETCH-LOGICAL-c4411-49556f00fc7efcf8e817b82a44fdf35b612d4897a6b73701aa6323d5b118e3b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.260340311$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.260340311$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6574255$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18588114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, Jun Sik</creatorcontrib><creatorcontrib>Chang, Ho Nam</creatorcontrib><title>Biotransformation of acrylonitrile to acrylamide using immobilized whole cells of Brevibacterium CH1 in a recycle fed-batch reactor</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Acrylamide was produced from acrylonitrile using immobilized Brevibacterium CH1 cells that were isolated from soil and found to possess nitrile hydratase activity. The reaction conditions and stability of the enzyme activity were studied. The conversion yield was nearly 100%, including a trace amount of acrylic acid. This strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of amidase toward acrylamide. A packed bed reactor was operated in a fed‐batch manner for acrylamide production of high concentration. The acrylonitrile concentration was maintained below 3% and the operating temperature at 4°C to minimize enzyme deactivation.</description><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNp9kE2LFDEQhoMo7rh69Co5CJ56TXU---gMuruwKMjKgpeQpBM32t1Zk27X8eofN8MMoydPRRVPVb08CD0HcgaEtK9tnM9aQSgjFOABWgHpZEPajjxEK0KIaCjv2hP0pJSvtZVKiMfoBBRXCoCt0O91THM2Uwkpj2aOacIpYOPydkhTnHMcPJ7TfmDG2Hu8lDh9wXEck41D_OV7fH-bKuX8MJTd8jr7H9EaN_sclxFvLgDHCRucvdu6CgbfN9bM7rZOKpXyU_QomKH4Z4d6ij69e3u9uWiuPpxfbt5cNY4xgIZ1nItASHDSBxeUVyCtag1joQ-UWwFtz1QnjbCSSgLGCNrSnlsA5akFeope7e_e5fR98WXWYyy72GbyaSlaUso6BVxWstmTLqdSsg_6LsfR5K0GonfaddWuj9or_-JwebGj7__SB88VeHkATHFmCNW4i-XICS5Zy3nF5B67r963_3-q15fX_yY4JI5l9j-PmyZ_06La4Prm_bn-yMjndtPdaEX_ABxdq0U</recordid><startdate>198907</startdate><enddate>198907</enddate><creator>Hwang, Jun Sik</creator><creator>Chang, Ho Nam</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198907</creationdate><title>Biotransformation of acrylonitrile to acrylamide using immobilized whole cells of Brevibacterium CH1 in a recycle fed-batch reactor</title><author>Hwang, Jun Sik ; Chang, Ho Nam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4411-49556f00fc7efcf8e817b82a44fdf35b612d4897a6b73701aa6323d5b118e3b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, Jun Sik</creatorcontrib><creatorcontrib>Chang, Ho Nam</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, Jun Sik</au><au>Chang, Ho Nam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biotransformation of acrylonitrile to acrylamide using immobilized whole cells of Brevibacterium CH1 in a recycle fed-batch reactor</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1989-07</date><risdate>1989</risdate><volume>34</volume><issue>3</issue><spage>380</spage><epage>386</epage><pages>380-386</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Acrylamide was produced from acrylonitrile using immobilized Brevibacterium CH1 cells that were isolated from soil and found to possess nitrile hydratase activity. The reaction conditions and stability of the enzyme activity were studied. The conversion yield was nearly 100%, including a trace amount of acrylic acid. This strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of amidase toward acrylamide. A packed bed reactor was operated in a fed‐batch manner for acrylamide production of high concentration. The acrylonitrile concentration was maintained below 3% and the operating temperature at 4°C to minimize enzyme deactivation.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18588114</pmid><doi>10.1002/bit.260340311</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3592 |
| ispartof | Biotechnology and bioengineering, 1989-07, Vol.34 (3), p.380-386 |
| issn | 0006-3592 1097-0290 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733498157 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies |
| title | Biotransformation of acrylonitrile to acrylamide using immobilized whole cells of Brevibacterium CH1 in a recycle fed-batch reactor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T03%3A25%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biotransformation%20of%20acrylonitrile%20to%20acrylamide%20using%20immobilized%20whole%20cells%20of%20Brevibacterium%20CH1%20in%20a%20recycle%20fed-batch%20reactor&rft.jtitle=Biotechnology%20and%20bioengineering&rft.au=Hwang,%20Jun%20Sik&rft.date=1989-07&rft.volume=34&rft.issue=3&rft.spage=380&rft.epage=386&rft.pages=380-386&rft.issn=0006-3592&rft.eissn=1097-0290&rft.coden=BIBIAU&rft_id=info:doi/10.1002/bit.260340311&rft_dat=%3Cproquest_cross%3E733498157%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733498157&rft_id=info:pmid/18588114&rfr_iscdi=true |