Rapid activation of phenylpropanoid metabolism in elicitor-treated hybrid poplar (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) suspension-cultured cells
Elicitor induction of phenylpropanoid metabolism was investigated in suspension-cultured cells of the fast-growing poplar hybrid (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) H11-11. Treatment of cells with polygalacturonic acid lyase or two fungal elicitors resulted in rapid and...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1992-02, Vol.98 (2), p.728-737 |
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| creator | De Sa, M.M Subramaniam, R Williams, F.E Douglas, C.J |
| description | Elicitor induction of phenylpropanoid metabolism was investigated in suspension-cultured cells of the fast-growing poplar hybrid (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) H11-11. Treatment of cells with polygalacturonic acid lyase or two fungal elicitors resulted in rapid and transient increases in extractable L-phenylalanine ammonia lyase and 4-coumarate:coenzyme A ligase enzyme activities. The substrate specificity of the inducible 4-coumarate:coenzyme A ligase enzyme activity appeared to differ from substrate specificity of 4-coumarate:coenzyme A ligase enzyme activity in untreated control cells. Large and transient increases in the accumulation of L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase mRNAs preceded the increases in enzyme activities and were detectable by 30 minutes after the start of elicitor treatment. Chalcone synthase, cinnamyl alcohol dehydrogenase, and coniferin beta-glucosidase enzyme activities were unaffected by the elicitors, but a large and transient increase in beta-glucosidase activity capable of hydrolyzing 4-nitrophenyl-beta-glucoside was observed. Subsequent to increases in L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase enzyme activities, cell wall-bound thioglycolic acid-extractable compounds accumulated in elicitor-treated cultures, and these cells exhibited strong staining with phloroglucinol, suggesting the accumulation of wall-bound phenolic compounds. |
| doi_str_mv | 10.1104/pp.98.2.728 |
| format | Article |
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Treatment of cells with polygalacturonic acid lyase or two fungal elicitors resulted in rapid and transient increases in extractable L-phenylalanine ammonia lyase and 4-coumarate:coenzyme A ligase enzyme activities. The substrate specificity of the inducible 4-coumarate:coenzyme A ligase enzyme activity appeared to differ from substrate specificity of 4-coumarate:coenzyme A ligase enzyme activity in untreated control cells. Large and transient increases in the accumulation of L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase mRNAs preceded the increases in enzyme activities and were detectable by 30 minutes after the start of elicitor treatment. Chalcone synthase, cinnamyl alcohol dehydrogenase, and coniferin beta-glucosidase enzyme activities were unaffected by the elicitors, but a large and transient increase in beta-glucosidase activity capable of hydrolyzing 4-nitrophenyl-beta-glucoside was observed. Subsequent to increases in L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase enzyme activities, cell wall-bound thioglycolic acid-extractable compounds accumulated in elicitor-treated cultures, and these cells exhibited strong staining with phloroglucinol, suggesting the accumulation of wall-bound phenolic compounds.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.98.2.728</identifier><identifier>PMID: 16668702</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACIDE PROPIONIQUE ; ACIDO PROPIONICO ; AGENT PATHOGENE ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; CAL ; CALLO ; callus ; Cell culture techniques ; cell suspension culture ; Cell walls ; Cellular metabolism ; COMPOSE PHENOLIQUE ; COMPUESTOS FENOLICOS ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; Cultured cells ; derivatives ; Enzyme activity ; Fundamental and applied biological sciences. Psychology ; Genetics and breeding of economic plants ; HIBRIDOS ; HYBRIDE ; hybrids ; induced resistance ; Lignin ; metabolism ; METABOLISME ; METABOLISMO ; ORGANISMOS PATOGENOS ; Parsley ; Pest resistance ; phenolic compounds ; Plant cells ; Plant pathogens ; Plants ; Populus balsamifera subsp. trichocarpa ; POPULUS DELTOIDES ; POPULUS TRICHOCARPA ; propionic acid ; RESISTANCE INDUITE ; RESISTENCIA INDUCIDA ; RNA ; Varietal selection. Specialized plant breeding, plant breeding aims</subject><ispartof>Plant physiology (Bethesda), 1992-02, Vol.98 (2), p.728-737</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-b28b1a0742b67e0d43e8ad6f1e71f9b4d5f300e054d82f9b226d6651e72f4c163</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4274152$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4274152$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5255641$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16668702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Sa, M.M</creatorcontrib><creatorcontrib>Subramaniam, R</creatorcontrib><creatorcontrib>Williams, F.E</creatorcontrib><creatorcontrib>Douglas, C.J</creatorcontrib><title>Rapid activation of phenylpropanoid metabolism in elicitor-treated hybrid poplar (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) suspension-cultured cells</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Elicitor induction of phenylpropanoid metabolism was investigated in suspension-cultured cells of the fast-growing poplar hybrid (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) H11-11. Treatment of cells with polygalacturonic acid lyase or two fungal elicitors resulted in rapid and transient increases in extractable L-phenylalanine ammonia lyase and 4-coumarate:coenzyme A ligase enzyme activities. The substrate specificity of the inducible 4-coumarate:coenzyme A ligase enzyme activity appeared to differ from substrate specificity of 4-coumarate:coenzyme A ligase enzyme activity in untreated control cells. Large and transient increases in the accumulation of L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase mRNAs preceded the increases in enzyme activities and were detectable by 30 minutes after the start of elicitor treatment. Chalcone synthase, cinnamyl alcohol dehydrogenase, and coniferin beta-glucosidase enzyme activities were unaffected by the elicitors, but a large and transient increase in beta-glucosidase activity capable of hydrolyzing 4-nitrophenyl-beta-glucoside was observed. Subsequent to increases in L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase enzyme activities, cell wall-bound thioglycolic acid-extractable compounds accumulated in elicitor-treated cultures, and these cells exhibited strong staining with phloroglucinol, suggesting the accumulation of wall-bound phenolic compounds.</description><subject>ACIDE PROPIONIQUE</subject><subject>ACIDO PROPIONICO</subject><subject>AGENT PATHOGENE</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>CAL</subject><subject>CALLO</subject><subject>callus</subject><subject>Cell culture techniques</subject><subject>cell suspension culture</subject><subject>Cell walls</subject><subject>Cellular metabolism</subject><subject>COMPOSE PHENOLIQUE</subject><subject>COMPUESTOS FENOLICOS</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>Cultured cells</subject><subject>derivatives</subject><subject>Enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics and breeding of economic plants</subject><subject>HIBRIDOS</subject><subject>HYBRIDE</subject><subject>hybrids</subject><subject>induced resistance</subject><subject>Lignin</subject><subject>metabolism</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>ORGANISMOS PATOGENOS</subject><subject>Parsley</subject><subject>Pest resistance</subject><subject>phenolic compounds</subject><subject>Plant cells</subject><subject>Plant pathogens</subject><subject>Plants</subject><subject>Populus balsamifera subsp. trichocarpa</subject><subject>POPULUS DELTOIDES</subject><subject>POPULUS TRICHOCARPA</subject><subject>propionic acid</subject><subject>RESISTANCE INDUITE</subject><subject>RESISTENCIA INDUCIDA</subject><subject>RNA</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNplkk-L1TAUxYsoznN05U5EsxD_IK1JmrTpUgYdhRFFZ8BduU1TX4a0iUkqvE_k1_Q-3nNcuErI-XHuzT23KB4yWjFGxZsQqk5VvGq5ulVsmKx5yaVQt4sNpXinSnUnxb2UrimlrGbibnHCmqZRLeWb4vdXCHYkoLP9Bdn6hfiJhK1Zdi5EH2DxqM4mw-CdTTOxCzHOapt9LHM0kM1ItrshIhV8cBDJyy8-rG5NJEert15DDEAufYwVeU7OI-zId_IXGY3LWMAk8gli2r4iaU3BLAn7KPXq8hrRXhvn0v3izgQumQfH87S4ev_u8uxDefH5_OPZ24tSC8lyOXA1MKCt4EPTGjqK2igYm4mZlk3dIEY51ZQaKsWoOD5w3oxNI1Hmk9CsqU-LFwdf_P3P1aTczzbtO4DF-DX1bV2LrsX5Ivn6QOroU4pm6kO0M8Rdz2i_D6YPoe9Uz3sMBuknR991mM34jz0mgcCzIwBJg5siLNqmG05yKRvBEHt8wK4TRnAjC94KJvcujw7yBL6HHxEdrr51TCkEUHz6v8hxJyhrJU6C1X8AFXWzzA</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>De Sa, M.M</creator><creator>Subramaniam, R</creator><creator>Williams, F.E</creator><creator>Douglas, C.J</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920201</creationdate><title>Rapid activation of phenylpropanoid metabolism in elicitor-treated hybrid poplar (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) suspension-cultured cells</title><author>De Sa, M.M ; Subramaniam, R ; Williams, F.E ; Douglas, C.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-b28b1a0742b67e0d43e8ad6f1e71f9b4d5f300e054d82f9b226d6651e72f4c163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACIDE PROPIONIQUE</topic><topic>ACIDO PROPIONICO</topic><topic>AGENT PATHOGENE</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>CAL</topic><topic>CALLO</topic><topic>callus</topic><topic>Cell culture techniques</topic><topic>cell suspension culture</topic><topic>Cell walls</topic><topic>Cellular metabolism</topic><topic>COMPOSE PHENOLIQUE</topic><topic>COMPUESTOS FENOLICOS</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>Cultured cells</topic><topic>derivatives</topic><topic>Enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics and breeding of economic plants</topic><topic>HIBRIDOS</topic><topic>HYBRIDE</topic><topic>hybrids</topic><topic>induced resistance</topic><topic>Lignin</topic><topic>metabolism</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>ORGANISMOS PATOGENOS</topic><topic>Parsley</topic><topic>Pest resistance</topic><topic>phenolic compounds</topic><topic>Plant cells</topic><topic>Plant pathogens</topic><topic>Plants</topic><topic>Populus balsamifera subsp. trichocarpa</topic><topic>POPULUS DELTOIDES</topic><topic>POPULUS TRICHOCARPA</topic><topic>propionic acid</topic><topic>RESISTANCE INDUITE</topic><topic>RESISTENCIA INDUCIDA</topic><topic>RNA</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Sa, M.M</creatorcontrib><creatorcontrib>Subramaniam, R</creatorcontrib><creatorcontrib>Williams, F.E</creatorcontrib><creatorcontrib>Douglas, C.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Sa, M.M</au><au>Subramaniam, R</au><au>Williams, F.E</au><au>Douglas, C.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid activation of phenylpropanoid metabolism in elicitor-treated hybrid poplar (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) suspension-cultured cells</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>98</volume><issue>2</issue><spage>728</spage><epage>737</epage><pages>728-737</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Elicitor induction of phenylpropanoid metabolism was investigated in suspension-cultured cells of the fast-growing poplar hybrid (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) H11-11. Treatment of cells with polygalacturonic acid lyase or two fungal elicitors resulted in rapid and transient increases in extractable L-phenylalanine ammonia lyase and 4-coumarate:coenzyme A ligase enzyme activities. The substrate specificity of the inducible 4-coumarate:coenzyme A ligase enzyme activity appeared to differ from substrate specificity of 4-coumarate:coenzyme A ligase enzyme activity in untreated control cells. Large and transient increases in the accumulation of L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase mRNAs preceded the increases in enzyme activities and were detectable by 30 minutes after the start of elicitor treatment. Chalcone synthase, cinnamyl alcohol dehydrogenase, and coniferin beta-glucosidase enzyme activities were unaffected by the elicitors, but a large and transient increase in beta-glucosidase activity capable of hydrolyzing 4-nitrophenyl-beta-glucoside was observed. Subsequent to increases in L-phenylalanine ammonia-lyase and 4-coumarate:coenzyme A ligase enzyme activities, cell wall-bound thioglycolic acid-extractable compounds accumulated in elicitor-treated cultures, and these cells exhibited strong staining with phloroglucinol, suggesting the accumulation of wall-bound phenolic compounds.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16668702</pmid><doi>10.1104/pp.98.2.728</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1992-02, Vol.98 (2), p.728-737 |
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| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ACIDE PROPIONIQUE ACIDO PROPIONICO AGENT PATHOGENE Agronomy. Soil science and plant productions Biological and medical sciences CAL CALLO callus Cell culture techniques cell suspension culture Cell walls Cellular metabolism COMPOSE PHENOLIQUE COMPUESTOS FENOLICOS CULTIVO DE CELULAS CULTURE DE CELLULE Cultured cells derivatives Enzyme activity Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants HIBRIDOS HYBRIDE hybrids induced resistance Lignin metabolism METABOLISME METABOLISMO ORGANISMOS PATOGENOS Parsley Pest resistance phenolic compounds Plant cells Plant pathogens Plants Populus balsamifera subsp. trichocarpa POPULUS DELTOIDES POPULUS TRICHOCARPA propionic acid RESISTANCE INDUITE RESISTENCIA INDUCIDA RNA Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Rapid activation of phenylpropanoid metabolism in elicitor-treated hybrid poplar (Populus trichocarpa Torr. & Gray X Populus deltoides Marsh) suspension-cultured cells |
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