Determination of H+/ATP stoichiometry for the plasma membrane H+-ATPase from red beet (Beta vulgaris L.) storage tissue
The H+/ATP stoichiometry was determined for the plasma membrane H+-ATPase from red beet (Beta vulgaris L., var Detroit Dark Red) storage tissue associated with native vesicles. The determination of H+/ATP stoichiometry utilized a kinetic approach where rates of H+ influx, estimated by three differen...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1991-01, Vol.95 (1), p.242-250 |
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| creator | Briskin, D.P. (University of Illinois, Urbana, IL) Reynolds-Niesman, I |
| description | The H+/ATP stoichiometry was determined for the plasma membrane H+-ATPase from red beet (Beta vulgaris L., var Detroit Dark Red) storage tissue associated with native vesicles. The determination of H+/ATP stoichiometry utilized a kinetic approach where rates of H+ influx, estimated by three different methods, were compared to rates of ATP hydrolysis measured by the coupled enzyme assay under identical conditions. These methods for estimating H+ influx were based upon either determining the initial rate of alkalinization of the external medium from pH 6.13, measuring the rate of vesicle H+ leakage from a steady-state pH gradient after stopping the H+-ATPase or utilizing a mathematical model which describes the net transport of H+ at any given point in time. When the rate of H+ influx estimated by each of these methods was compared to the rate of ATP hydrolysis, a H+/ATP stoichiometry of about 1 was observed. In consideration of the maximum free energy available from ATP hydrolysis (delta G(ATP)), this value for H+/ATP stoichiometry is sufficient to account for the magnitude of the proton electrochemical gradient observed across the plasms membrane in vivo |
| doi_str_mv | 10.1104/pp.95.1.242 |
| format | Article |
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(University of Illinois, Urbana, IL) ; Reynolds-Niesman, I</creator><creatorcontrib>Briskin, D.P. (University of Illinois, Urbana, IL) ; Reynolds-Niesman, I</creatorcontrib><description>The H+/ATP stoichiometry was determined for the plasma membrane H+-ATPase from red beet (Beta vulgaris L., var Detroit Dark Red) storage tissue associated with native vesicles. The determination of H+/ATP stoichiometry utilized a kinetic approach where rates of H+ influx, estimated by three different methods, were compared to rates of ATP hydrolysis measured by the coupled enzyme assay under identical conditions. These methods for estimating H+ influx were based upon either determining the initial rate of alkalinization of the external medium from pH 6.13, measuring the rate of vesicle H+ leakage from a steady-state pH gradient after stopping the H+-ATPase or utilizing a mathematical model which describes the net transport of H+ at any given point in time. When the rate of H+ influx estimated by each of these methods was compared to the rate of ATP hydrolysis, a H+/ATP stoichiometry of about 1 was observed. In consideration of the maximum free energy available from ATP hydrolysis (delta G(ATP)), this value for H+/ATP stoichiometry is sufficient to account for the magnitude of the proton electrochemical gradient observed across the plasms membrane in vivo</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.95.1.242</identifier><identifier>PMID: 16667959</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Adenosine triphosphatases ; ADENOSINE TRIPHOSPHATE ; ADENOSINTRIFOSFATO ; Beets ; BETA VULGARIS ; Biological and medical sciences ; Cell membranes ; Cell physiology ; Dyes ; ENERGIA ; ENERGIE ; Estimation methods ; ESTRUCTURA CELULAR ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; HIDROGENO ; HIDROLASAS ; HYDROGENE ; HYDROLASE ; Hydrolysis ; ION ; IONES ; Kinetics ; Membranes and Bioenergetics ; Plant physiology and development ; Plasma membrane and permeation ; Protons ; RACINE ; RAICES ; Stoichiometry ; STRUCTURE CELLULAIRE</subject><ispartof>Plant physiology (Bethesda), 1991-01, Vol.95 (1), p.242-250</ispartof><rights>Copyright 1991 American Society of Plant Physiologists</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-acd037e36ba7632fd40d08330f771ba6800d5d8f9ad677bf39113663117726b63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4273370$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4273370$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,777,781,800,882,4010,27904,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19575528$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16667959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Briskin, D.P. (University of Illinois, Urbana, IL)</creatorcontrib><creatorcontrib>Reynolds-Niesman, I</creatorcontrib><title>Determination of H+/ATP stoichiometry for the plasma membrane H+-ATPase from red beet (Beta vulgaris L.) storage tissue</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The H+/ATP stoichiometry was determined for the plasma membrane H+-ATPase from red beet (Beta vulgaris L., var Detroit Dark Red) storage tissue associated with native vesicles. The determination of H+/ATP stoichiometry utilized a kinetic approach where rates of H+ influx, estimated by three different methods, were compared to rates of ATP hydrolysis measured by the coupled enzyme assay under identical conditions. These methods for estimating H+ influx were based upon either determining the initial rate of alkalinization of the external medium from pH 6.13, measuring the rate of vesicle H+ leakage from a steady-state pH gradient after stopping the H+-ATPase or utilizing a mathematical model which describes the net transport of H+ at any given point in time. When the rate of H+ influx estimated by each of these methods was compared to the rate of ATP hydrolysis, a H+/ATP stoichiometry of about 1 was observed. In consideration of the maximum free energy available from ATP hydrolysis (delta G(ATP)), this value for H+/ATP stoichiometry is sufficient to account for the magnitude of the proton electrochemical gradient observed across the plasms membrane in vivo</description><subject>Adenosine triphosphatases</subject><subject>ADENOSINE TRIPHOSPHATE</subject><subject>ADENOSINTRIFOSFATO</subject><subject>Beets</subject><subject>BETA VULGARIS</subject><subject>Biological and medical sciences</subject><subject>Cell membranes</subject><subject>Cell physiology</subject><subject>Dyes</subject><subject>ENERGIA</subject><subject>ENERGIE</subject><subject>Estimation methods</subject><subject>ESTRUCTURA CELULAR</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIDROGENO</subject><subject>HIDROLASAS</subject><subject>HYDROGENE</subject><subject>HYDROLASE</subject><subject>Hydrolysis</subject><subject>ION</subject><subject>IONES</subject><subject>Kinetics</subject><subject>Membranes and Bioenergetics</subject><subject>Plant physiology and development</subject><subject>Plasma membrane and permeation</subject><subject>Protons</subject><subject>RACINE</subject><subject>RAICES</subject><subject>Stoichiometry</subject><subject>STRUCTURE CELLULAIRE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNpVkc1v1DAQxSMEokvhxA0h5AsCVCW149hOLkilfBRpJZBoz9YkGe-6SuLUdor63-NlVy2cbOn99GbmvSx7yWjBGK1O57loRMGKsiofZSsmeJmXoqofZytK05_WdXOUPQvhmlLKOKueZkdMSqka0ayy358xoh_tBNG6iThDLk5Ozy5_khCd7bbWjRj9HTHOk7hFMg8QRiAjjq2HCROcJxgCEuPdSDz2pEWM5P0njEBul2ED3gayLj7sDD1skEQbwoLPsycGhoAvDu9xdvX1y-X5Rb7-8e37-dk676qaxxy6nnKFXLagJC9NX9Ge1pxToxRrQdaU9qKvTQO9VKo1vGGMS8kZU6qUreTH2ce977y0I_YdTtHDoGdvR_B32oHV_yuT3eqNu9WMKiUYTwbvDgbe3SwYoh5t6HAY0vluCVpxXjWClnUiT_Zk510IHs39FEb1rik9z7oRmunUVKLf_LvYA3uoJgFvDwCEDgaT8u5seOAaoYT4O_b1nrveJXyvV2XaTNEkv9rLBpyGTWpDX_1KKYmSC_4HP8etBA</recordid><startdate>199101</startdate><enddate>199101</enddate><creator>Briskin, D.P. (University of Illinois, Urbana, IL)</creator><creator>Reynolds-Niesman, I</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199101</creationdate><title>Determination of H+/ATP stoichiometry for the plasma membrane H+-ATPase from red beet (Beta vulgaris L.) storage tissue</title><author>Briskin, D.P. (University of Illinois, Urbana, IL) ; Reynolds-Niesman, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-acd037e36ba7632fd40d08330f771ba6800d5d8f9ad677bf39113663117726b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adenosine triphosphatases</topic><topic>ADENOSINE TRIPHOSPHATE</topic><topic>ADENOSINTRIFOSFATO</topic><topic>Beets</topic><topic>BETA VULGARIS</topic><topic>Biological and medical sciences</topic><topic>Cell membranes</topic><topic>Cell physiology</topic><topic>Dyes</topic><topic>ENERGIA</topic><topic>ENERGIE</topic><topic>Estimation methods</topic><topic>ESTRUCTURA CELULAR</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIDROGENO</topic><topic>HIDROLASAS</topic><topic>HYDROGENE</topic><topic>HYDROLASE</topic><topic>Hydrolysis</topic><topic>ION</topic><topic>IONES</topic><topic>Kinetics</topic><topic>Membranes and Bioenergetics</topic><topic>Plant physiology and development</topic><topic>Plasma membrane and permeation</topic><topic>Protons</topic><topic>RACINE</topic><topic>RAICES</topic><topic>Stoichiometry</topic><topic>STRUCTURE CELLULAIRE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Briskin, D.P. (University of Illinois, Urbana, IL)</creatorcontrib><creatorcontrib>Reynolds-Niesman, I</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Briskin, D.P. (University of Illinois, Urbana, IL)</au><au>Reynolds-Niesman, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of H+/ATP stoichiometry for the plasma membrane H+-ATPase from red beet (Beta vulgaris L.) storage tissue</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1991-01</date><risdate>1991</risdate><volume>95</volume><issue>1</issue><spage>242</spage><epage>250</epage><pages>242-250</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The H+/ATP stoichiometry was determined for the plasma membrane H+-ATPase from red beet (Beta vulgaris L., var Detroit Dark Red) storage tissue associated with native vesicles. The determination of H+/ATP stoichiometry utilized a kinetic approach where rates of H+ influx, estimated by three different methods, were compared to rates of ATP hydrolysis measured by the coupled enzyme assay under identical conditions. These methods for estimating H+ influx were based upon either determining the initial rate of alkalinization of the external medium from pH 6.13, measuring the rate of vesicle H+ leakage from a steady-state pH gradient after stopping the H+-ATPase or utilizing a mathematical model which describes the net transport of H+ at any given point in time. When the rate of H+ influx estimated by each of these methods was compared to the rate of ATP hydrolysis, a H+/ATP stoichiometry of about 1 was observed. In consideration of the maximum free energy available from ATP hydrolysis (delta G(ATP)), this value for H+/ATP stoichiometry is sufficient to account for the magnitude of the proton electrochemical gradient observed across the plasms membrane in vivo</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16667959</pmid><doi>10.1104/pp.95.1.242</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | Adenosine triphosphatases ADENOSINE TRIPHOSPHATE ADENOSINTRIFOSFATO Beets BETA VULGARIS Biological and medical sciences Cell membranes Cell physiology Dyes ENERGIA ENERGIE Estimation methods ESTRUCTURA CELULAR Fluorescence Fundamental and applied biological sciences. Psychology HIDROGENO HIDROLASAS HYDROGENE HYDROLASE Hydrolysis ION IONES Kinetics Membranes and Bioenergetics Plant physiology and development Plasma membrane and permeation Protons RACINE RAICES Stoichiometry STRUCTURE CELLULAIRE |
| title | Determination of H+/ATP stoichiometry for the plasma membrane H+-ATPase from red beet (Beta vulgaris L.) storage tissue |
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