Purification and developmental analysis of the major anionic peroxidase from the seed coat of Glycine max

We show that the majority of peroxidase activity in soybean (Glycine max var Williams 82) seeds is localized to the seed coat. A single isozyme is responsible for this activity and has been purified to electrophoretic homogeneity by successive chromatography on DEAE Sepharose Fast Flow, concanavalin...

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Veröffentlicht in:Plant physiology (Bethesda) 1991-05, Vol.96 (1), p.214-220
Hauptverfasser: Gillikin, J.W. (Bowling Green State University, Bowling Green, OH), Graham, J.S
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creator Gillikin, J.W. (Bowling Green State University, Bowling Green, OH)
Graham, J.S
description We show that the majority of peroxidase activity in soybean (Glycine max var Williams 82) seeds is localized to the seed coat. A single isozyme is responsible for this activity and has been purified to electrophoretic homogeneity by successive chromatography on DEAE Sepharose Fast Flow, concanavalin A-Sepharose, and Sephadex G-75. The peroxidase exhibits a pl of 4.1, an apparent molecular mass of 37 kilodaltons, and has properties characteristic of a glycoprotein. The enzyme begins to accumulate approximately 21 days after anthesis and continues to do so throughout the maturation of the seed coat where it can represent at least 5% of the soluble protein in dry seed coats. Due to its localization in the seed, we propose that this isozyme may play a role in the hardening of the seed cost
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source JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ANION
ANIONES
Cell walls
Cellulose nitrate
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CONTENIDO PROTEICO
DURCISSEMENT
ENDURECIMIENTO
Enzymes
Gels
GLYCINE MAX
ISOENZIMAS
ISOENZYME
Lignin
PEROXIDASAS
peroxidase
PEROXYDASE
Plant tissues
Plants
Polymerization
PROPIEDADES FISICO-QUIMICAS
PROPRIETE PHYSICOCHIMIQUE
PROTEINAS
PROTEINE
PURIFICACION
PURIFICATION
seeds
SEMENCE
SEMILLAS
Soybeans
TENEUR EN PROTEINES
TESTA
title Purification and developmental analysis of the major anionic peroxidase from the seed coat of Glycine max
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