Response of Cytosolic-Isozyme and Plastid-Isozyme Levels of 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthase to Physiological State of Nicotiana silvestris in Suspension Culture
Two isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase are partitioned into plastid (DS-Mn) and cytosolic (DS-Co) compartments of at least several higher plants (RA Jensen 1986 Rec Adv Phytochem 20: 257-258). Differential variation of isozyme levels and in the timing of their expressio...
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| Veröffentlicht in: | Plant Physiol.; (United States) 1987-03, Vol.83 (3), p.479-482 |
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| creator | Robert J. Ganson Jensen, Roy A. |
| description | Two isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase are partitioned into plastid (DS-Mn) and cytosolic (DS-Co) compartments of at least several higher plants (RA Jensen 1986 Rec Adv Phytochem 20: 257-258). Differential variation of isozyme levels and in the timing of their expression was observed during growth of Nicotiana silvestris in suspension culture. The ratio of DS-Co to DS-Mn varied about fivefold in comparison of the different physiological stages of growth. Cultures maintained in exponential phase for >10 generations (EE cells) possessed balanced-growth properties and did not exhibit the considerable variation of isozyme levels found during the initial 2 to 3 generations of exponential growth (E cells) that followed subculture of stationary-phase cultures. The plastid isozyme level declined substantially in stationary phase, responded immediately to subculture, and reached a peak in early exponential growth similar to the steady-state level of DS-Mn in EE cells. In contrast, the cytosolic isozyme level peaked in late exponential growth. A recent history of stationary-phase physiology appeared to foster elevated synthesis of DS-Co since the steady-state level of DS-Co in EE cells was much lower than in E cells. |
| doi_str_mv | 10.1104/pp.83.3.479 |
| format | Article |
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Ganson ; Jensen, Roy A.</creator><creatorcontrib>Robert J. Ganson ; Jensen, Roy A. ; State Univ. of New York, Binghamton</creatorcontrib><description>Two isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase are partitioned into plastid (DS-Mn) and cytosolic (DS-Co) compartments of at least several higher plants (RA Jensen 1986 Rec Adv Phytochem 20: 257-258). Differential variation of isozyme levels and in the timing of their expression was observed during growth of Nicotiana silvestris in suspension culture. The ratio of DS-Co to DS-Mn varied about fivefold in comparison of the different physiological stages of growth. Cultures maintained in exponential phase for >10 generations (EE cells) possessed balanced-growth properties and did not exhibit the considerable variation of isozyme levels found during the initial 2 to 3 generations of exponential growth (E cells) that followed subculture of stationary-phase cultures. The plastid isozyme level declined substantially in stationary phase, responded immediately to subculture, and reached a peak in early exponential growth similar to the steady-state level of DS-Mn in EE cells. In contrast, the cytosolic isozyme level peaked in late exponential growth. A recent history of stationary-phase physiology appeared to foster elevated synthesis of DS-Co since the steady-state level of DS-Co in EE cells was much lower than in E cells.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.83.3.479</identifier><identifier>PMID: 16665275</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>550200 - Biochemistry ; Agronomy. Soil science and plant productions ; Analytical, structural and metabolic biochemistry ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; CELL CULTURES ; Cell growth ; Cell physiology ; Communications ; Cultured cells ; Economic plant physiology ; ENZYME ACTIVITY ; ENZYMES ; Enzymes and enzyme inhibitors ; ESTERASES ; Fundamental and applied biological sciences. Psychology ; Geometric growth ; GROWTH ; HYDROLASES ; L cells ; Ligases ; NICOTIANA ; Nutrition. Photosynthesis. Respiration. 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Ganson</creatorcontrib><creatorcontrib>Jensen, Roy A.</creatorcontrib><creatorcontrib>State Univ. of New York, Binghamton</creatorcontrib><title>Response of Cytosolic-Isozyme and Plastid-Isozyme Levels of 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthase to Physiological State of Nicotiana silvestris in Suspension Culture</title><title>Plant Physiol.; (United States)</title><addtitle>Plant Physiol</addtitle><description>Two isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase are partitioned into plastid (DS-Mn) and cytosolic (DS-Co) compartments of at least several higher plants (RA Jensen 1986 Rec Adv Phytochem 20: 257-258). Differential variation of isozyme levels and in the timing of their expression was observed during growth of Nicotiana silvestris in suspension culture. The ratio of DS-Co to DS-Mn varied about fivefold in comparison of the different physiological stages of growth. Cultures maintained in exponential phase for >10 generations (EE cells) possessed balanced-growth properties and did not exhibit the considerable variation of isozyme levels found during the initial 2 to 3 generations of exponential growth (E cells) that followed subculture of stationary-phase cultures. The plastid isozyme level declined substantially in stationary phase, responded immediately to subculture, and reached a peak in early exponential growth similar to the steady-state level of DS-Mn in EE cells. In contrast, the cytosolic isozyme level peaked in late exponential growth. A recent history of stationary-phase physiology appeared to foster elevated synthesis of DS-Co since the steady-state level of DS-Co in EE cells was much lower than in E cells.</description><subject>550200 - Biochemistry</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>CELL CULTURES</subject><subject>Cell growth</subject><subject>Cell physiology</subject><subject>Communications</subject><subject>Cultured cells</subject><subject>Economic plant physiology</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>Enzymes and enzyme inhibitors</subject><subject>ESTERASES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geometric growth</subject><subject>GROWTH</subject><subject>HYDROLASES</subject><subject>L cells</subject><subject>Ligases</subject><subject>NICOTIANA</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>PHOSPHATASES</subject><subject>PLANT CELLS</subject><subject>PLANTS</subject><subject>Population growth</subject><subject>Steepest descent method</subject><subject>Subcultures</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpFkUGP0zAQhSMEYsvCiStCFkLigFLsOE6c46rssitVUFE4W44zoV65djbjrAj_i_-Hq1bl5JHf5zfjeVn2mtElY7T8NAxLyZd8WdbNk2zBBC_yQpTyabagNNVUyuYie4F4TyllnJXPswtWVZUoarHI_n4HHIJHIKEnqzkGDM6a_A7Dn3kPRPuObJzGaLvz3RoeweGB5_lnCL_nvMuvRt1aH_JbGOLkkonXEUidb3YBh92h3s4-7nTqEwPZ7Ga0wYVf1mhHtvGgJ7uv1oRotdcErXsEjKNFYj3ZTjiATy88WU0uTiO8zJ712iG8Op2X2c-b6x-r23z97cvd6mqdG17VMe-6ltVdowFAt1waUQrTFrTlpWS04kUnisLURnZ9LbioWto1zOi-l5oJk1R-mb07-oa0AYXGRjA7E7wHE1VV8qYRRYI-HKFhDA9TGlvtLRpwTnsIE6qa87IpuJSJ_HgkzRgQR-jVMNq9HmfFqDpkqYZBSa64Slkm-u3Jd2r30P1nT-El4P0J0JgW2Y_aG4tnrk6_bPjB580Ru8cYxrNcFjUt0_j_AG2rtDA</recordid><startdate>19870301</startdate><enddate>19870301</enddate><creator>Robert J. Ganson</creator><creator>Jensen, Roy A.</creator><general>American Society of Plant Physiologists</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19870301</creationdate><title>Response of Cytosolic-Isozyme and Plastid-Isozyme Levels of 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthase to Physiological State of Nicotiana silvestris in Suspension Culture</title><author>Robert J. Ganson ; Jensen, Roy A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-ddb17d9aeeeab38c545cb20b34810632d522c7c8df75356b0d91caff8a15c2d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>550200 - Biochemistry</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>CELL CULTURES</topic><topic>Cell growth</topic><topic>Cell physiology</topic><topic>Communications</topic><topic>Cultured cells</topic><topic>Economic plant physiology</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>Enzymes and enzyme inhibitors</topic><topic>ESTERASES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geometric growth</topic><topic>GROWTH</topic><topic>HYDROLASES</topic><topic>L cells</topic><topic>Ligases</topic><topic>NICOTIANA</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>PHOSPHATASES</topic><topic>PLANT CELLS</topic><topic>PLANTS</topic><topic>Population growth</topic><topic>Steepest descent method</topic><topic>Subcultures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robert J. Ganson</creatorcontrib><creatorcontrib>Jensen, Roy A.</creatorcontrib><creatorcontrib>State Univ. of New York, Binghamton</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Plant Physiol.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robert J. Ganson</au><au>Jensen, Roy A.</au><aucorp>State Univ. of New York, Binghamton</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Response of Cytosolic-Isozyme and Plastid-Isozyme Levels of 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthase to Physiological State of Nicotiana silvestris in Suspension Culture</atitle><jtitle>Plant Physiol.; (United States)</jtitle><addtitle>Plant Physiol</addtitle><date>1987-03-01</date><risdate>1987</risdate><volume>83</volume><issue>3</issue><spage>479</spage><epage>482</epage><pages>479-482</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Two isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase are partitioned into plastid (DS-Mn) and cytosolic (DS-Co) compartments of at least several higher plants (RA Jensen 1986 Rec Adv Phytochem 20: 257-258). Differential variation of isozyme levels and in the timing of their expression was observed during growth of Nicotiana silvestris in suspension culture. The ratio of DS-Co to DS-Mn varied about fivefold in comparison of the different physiological stages of growth. Cultures maintained in exponential phase for >10 generations (EE cells) possessed balanced-growth properties and did not exhibit the considerable variation of isozyme levels found during the initial 2 to 3 generations of exponential growth (E cells) that followed subculture of stationary-phase cultures. The plastid isozyme level declined substantially in stationary phase, responded immediately to subculture, and reached a peak in early exponential growth similar to the steady-state level of DS-Mn in EE cells. In contrast, the cytosolic isozyme level peaked in late exponential growth. A recent history of stationary-phase physiology appeared to foster elevated synthesis of DS-Co since the steady-state level of DS-Co in EE cells was much lower than in E cells.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16665275</pmid><doi>10.1104/pp.83.3.479</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 550200 - Biochemistry Agronomy. Soil science and plant productions Analytical, structural and metabolic biochemistry BASIC BIOLOGICAL SCIENCES Biological and medical sciences CELL CULTURES Cell growth Cell physiology Communications Cultured cells Economic plant physiology ENZYME ACTIVITY ENZYMES Enzymes and enzyme inhibitors ESTERASES Fundamental and applied biological sciences. Psychology Geometric growth GROWTH HYDROLASES L cells Ligases NICOTIANA Nutrition. Photosynthesis. Respiration. Metabolism PHOSPHATASES PLANT CELLS PLANTS Population growth Steepest descent method Subcultures |
| title | Response of Cytosolic-Isozyme and Plastid-Isozyme Levels of 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate Synthase to Physiological State of Nicotiana silvestris in Suspension Culture |
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