Preparation and properties of mitochondria from cowpea nodules
Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had simila...
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Veröffentlicht in: | Plant physiology (Bethesda) 1986-08, Vol.81 (4), p.1092-1096 |
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description | Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O2 uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD+-malic enzyme which was 12-fold lower in the mitochondria from nodules. |
doi_str_mv | 10.1104/pp.81.4.1092 |
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Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O2 uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD+-malic enzyme which was 12-fold lower in the mitochondria from nodules.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.81.4.1092</identifier><identifier>PMID: 16664949</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Bacteroids ; Biological and medical sciences ; Cell structures and functions ; Centrifugation ; CHEMICOPHYSICAL PROPERTIES ; Enzymes ; EPURATION ; FONCTION PHYSIOLOGIQUE ; FORMATION DE NODOSITES ; FUNCION FISIOLOGICA ; Fundamental and applied biological sciences. Psychology ; Hypocotyls ; ISOLATION TECHNIQUE ; MITOCHONDRIA ; Mitochondria and cell respiration ; MITOCHONDRIE ; MITOCONDRIA ; Molecular and cellular biology ; NODULACION ; Nodules ; Oxidation ; OXIDOREDUCTASES ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; percoll gradient ; PHYSIOLOGICAL FUNCTIONS ; Plants ; PROPIEDADES FISICO-QUIMICAS ; PROPRIETE PHYSICO-CHIMIQUE ; PURIFICACION ; PURIFICATION ; RESPIRACION ; RESPIRATION ; ROOT NODULATION ; Soybeans ; TECHNIQUE DE L'ISOLEMENT ; TECNICAS DE AISLAMIENTO ; VIGNA UNGUICULATA</subject><ispartof>Plant physiology (Bethesda), 1986-08, Vol.81 (4), p.1092-1096</ispartof><rights>Copyright 1986 American Society of Plant Physiologists</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-a5ba50d738d10279390b5fdde7e267621efe06cfac5d76edd714f4a49f7cf70b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4270109$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4270109$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8098366$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16664949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rawsthorne, S</creatorcontrib><creatorcontrib>LaRue, T.A</creatorcontrib><title>Preparation and properties of mitochondria from cowpea nodules</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O2 uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD+-malic enzyme which was 12-fold lower in the mitochondria from nodules.</description><subject>Bacteroids</subject><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>Centrifugation</subject><subject>CHEMICOPHYSICAL PROPERTIES</subject><subject>Enzymes</subject><subject>EPURATION</subject><subject>FONCTION PHYSIOLOGIQUE</subject><subject>FORMATION DE NODOSITES</subject><subject>FUNCION FISIOLOGICA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypocotyls</subject><subject>ISOLATION TECHNIQUE</subject><subject>MITOCHONDRIA</subject><subject>Mitochondria and cell respiration</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>Molecular and cellular biology</subject><subject>NODULACION</subject><subject>Nodules</subject><subject>Oxidation</subject><subject>OXIDOREDUCTASES</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>percoll gradient</subject><subject>PHYSIOLOGICAL FUNCTIONS</subject><subject>Plants</subject><subject>PROPIEDADES FISICO-QUIMICAS</subject><subject>PROPRIETE PHYSICO-CHIMIQUE</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>RESPIRACION</subject><subject>RESPIRATION</subject><subject>ROOT NODULATION</subject><subject>Soybeans</subject><subject>TECHNIQUE DE L'ISOLEMENT</subject><subject>TECNICAS DE AISLAMIENTO</subject><subject>VIGNA UNGUICULATA</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNpFkM1LHEEUxJugxHWTW04iMgchF3fzerqnPy5CkCQKCwqJ5-Ztf-jIzHTbPUPIf59ZdtHTK6gfVY8i5AuFNaXAv6W0VnTN1xR0_YEsaMPqVd1wdUQWALMGpfQJOS3lBQAoo_wjOaFCCK65XpDrh-wTZhzbOFQ4uCrlmHweW1-qGKq-HaN9joPLLVYhx76y8W_yWA3RTZ0vn8hxwK74z4e7JI8_f_y5uV1t7n_d3XzfrCwTbFxhs8UGnGTKUailZhq2TXDOS18LKWrqgwdhA9rGSeGdk5QHjlwHaYOELVuSr_vc-b3XyZfR9G2xvutw8HEqRjLGNahazOTVnrQ5lpJ9MCm3PeZ_hoLZDWZSMooabnaDzfjFIXja9t69w4eFZuDyAGCx2IWMg23LG6dAKyZ2ted77KWMMb_ZvJYw98z22d4OGA0-5Tnh8beSwJRg7D-0XYUF</recordid><startdate>19860801</startdate><enddate>19860801</enddate><creator>Rawsthorne, S</creator><creator>LaRue, T.A</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860801</creationdate><title>Preparation and properties of mitochondria from cowpea nodules</title><author>Rawsthorne, S ; LaRue, T.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-a5ba50d738d10279390b5fdde7e267621efe06cfac5d76edd714f4a49f7cf70b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Bacteroids</topic><topic>Biological and medical sciences</topic><topic>Cell structures and functions</topic><topic>Centrifugation</topic><topic>CHEMICOPHYSICAL PROPERTIES</topic><topic>Enzymes</topic><topic>EPURATION</topic><topic>FONCTION PHYSIOLOGIQUE</topic><topic>FORMATION DE NODOSITES</topic><topic>FUNCION FISIOLOGICA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypocotyls</topic><topic>ISOLATION TECHNIQUE</topic><topic>MITOCHONDRIA</topic><topic>Mitochondria and cell respiration</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>Molecular and cellular biology</topic><topic>NODULACION</topic><topic>Nodules</topic><topic>Oxidation</topic><topic>OXIDOREDUCTASES</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>percoll gradient</topic><topic>PHYSIOLOGICAL FUNCTIONS</topic><topic>Plants</topic><topic>PROPIEDADES FISICO-QUIMICAS</topic><topic>PROPRIETE PHYSICO-CHIMIQUE</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>RESPIRACION</topic><topic>RESPIRATION</topic><topic>ROOT NODULATION</topic><topic>Soybeans</topic><topic>TECHNIQUE DE L'ISOLEMENT</topic><topic>TECNICAS DE AISLAMIENTO</topic><topic>VIGNA UNGUICULATA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rawsthorne, S</creatorcontrib><creatorcontrib>LaRue, T.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rawsthorne, S</au><au>LaRue, T.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and properties of mitochondria from cowpea nodules</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1986-08-01</date><risdate>1986</risdate><volume>81</volume><issue>4</issue><spage>1092</spage><epage>1096</epage><pages>1092-1096</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O2 uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD+-malic enzyme which was 12-fold lower in the mitochondria from nodules.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16664949</pmid><doi>10.1104/pp.81.4.1092</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection |
subjects | Bacteroids Biological and medical sciences Cell structures and functions Centrifugation CHEMICOPHYSICAL PROPERTIES Enzymes EPURATION FONCTION PHYSIOLOGIQUE FORMATION DE NODOSITES FUNCION FISIOLOGICA Fundamental and applied biological sciences. Psychology Hypocotyls ISOLATION TECHNIQUE MITOCHONDRIA Mitochondria and cell respiration MITOCHONDRIE MITOCONDRIA Molecular and cellular biology NODULACION Nodules Oxidation OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE percoll gradient PHYSIOLOGICAL FUNCTIONS Plants PROPIEDADES FISICO-QUIMICAS PROPRIETE PHYSICO-CHIMIQUE PURIFICACION PURIFICATION RESPIRACION RESPIRATION ROOT NODULATION Soybeans TECHNIQUE DE L'ISOLEMENT TECNICAS DE AISLAMIENTO VIGNA UNGUICULATA |
title | Preparation and properties of mitochondria from cowpea nodules |
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