Preparation and properties of mitochondria from cowpea nodules

Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had simila...

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Veröffentlicht in:Plant physiology (Bethesda) 1986-08, Vol.81 (4), p.1092-1096
Hauptverfasser: Rawsthorne, S, LaRue, T.A
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description Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O2 uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD+-malic enzyme which was 12-fold lower in the mitochondria from nodules.
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Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O2 uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD+-malic enzyme which was 12-fold lower in the mitochondria from nodules.</description><subject>Bacteroids</subject><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>Centrifugation</subject><subject>CHEMICOPHYSICAL PROPERTIES</subject><subject>Enzymes</subject><subject>EPURATION</subject><subject>FONCTION PHYSIOLOGIQUE</subject><subject>FORMATION DE NODOSITES</subject><subject>FUNCION FISIOLOGICA</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Hypocotyls</topic><topic>ISOLATION TECHNIQUE</topic><topic>MITOCHONDRIA</topic><topic>Mitochondria and cell respiration</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>Molecular and cellular biology</topic><topic>NODULACION</topic><topic>Nodules</topic><topic>Oxidation</topic><topic>OXIDOREDUCTASES</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>percoll gradient</topic><topic>PHYSIOLOGICAL FUNCTIONS</topic><topic>Plants</topic><topic>PROPIEDADES FISICO-QUIMICAS</topic><topic>PROPRIETE PHYSICO-CHIMIQUE</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>RESPIRACION</topic><topic>RESPIRATION</topic><topic>ROOT NODULATION</topic><topic>Soybeans</topic><topic>TECHNIQUE DE L'ISOLEMENT</topic><topic>TECNICAS DE AISLAMIENTO</topic><topic>VIGNA UNGUICULATA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rawsthorne, S</creatorcontrib><creatorcontrib>LaRue, T.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rawsthorne, S</au><au>LaRue, T.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and properties of mitochondria from cowpea nodules</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1986-08-01</date><risdate>1986</risdate><volume>81</volume><issue>4</issue><spage>1092</spage><epage>1096</epage><pages>1092-1096</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O2 uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD+-malic enzyme which was 12-fold lower in the mitochondria from nodules.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16664949</pmid><doi>10.1104/pp.81.4.1092</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection
subjects Bacteroids
Biological and medical sciences
Cell structures and functions
Centrifugation
CHEMICOPHYSICAL PROPERTIES
Enzymes
EPURATION
FONCTION PHYSIOLOGIQUE
FORMATION DE NODOSITES
FUNCION FISIOLOGICA
Fundamental and applied biological sciences. Psychology
Hypocotyls
ISOLATION TECHNIQUE
MITOCHONDRIA
Mitochondria and cell respiration
MITOCHONDRIE
MITOCONDRIA
Molecular and cellular biology
NODULACION
Nodules
Oxidation
OXIDOREDUCTASES
OXIDORREDUCTASAS
OXYDOREDUCTASE
percoll gradient
PHYSIOLOGICAL FUNCTIONS
Plants
PROPIEDADES FISICO-QUIMICAS
PROPRIETE PHYSICO-CHIMIQUE
PURIFICACION
PURIFICATION
RESPIRACION
RESPIRATION
ROOT NODULATION
Soybeans
TECHNIQUE DE L'ISOLEMENT
TECNICAS DE AISLAMIENTO
VIGNA UNGUICULATA
title Preparation and properties of mitochondria from cowpea nodules
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