Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels
An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, α-amylase, and β-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through...
Gespeichert in:
| Veröffentlicht in: | Plant physiology (Bethesda) 1984-05, Vol.75 (1), p.278-280 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 280 |
|---|---|
| container_issue | 1 |
| container_start_page | 278 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 75 |
| creator | KAKEFUDA, G DUKE, S. H |
| description | An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, α-amylase, and β-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the transfer gel to its characteristic limit dextrin as it moves through the gel. Various limit dextrins in the starch gel are identified by their characteristic color development in KI/I solution. Isozymes of starch debranching enzyme, α-amylase, and β-amylase can be easily identified in the same gel. |
| doi_str_mv | 10.1104/pp.75.1.278 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_733487867</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4268663</jstor_id><sourcerecordid>4268663</sourcerecordid><originalsourceid>FETCH-LOGICAL-c371t-4dcf32e885d528e042c1f0ff58f55b8a906ec0808f5131ede2268a67dad33f6f3</originalsourceid><addsrcrecordid>eNp9kTtvFDEUhS0EIkugokXIBYIC7eLH-DFlFJYQKRIpltpy7GutI894sGeLScNfx2FXoaO6vsefj3x0EHpLyYZS0n2Zpo0SG7phSj9DKyo4WzPR6edoRUg7E637M_Sq1ntCCOW0e4nOqJSSi16s0O9tAjeXPO1zgTk6vCt2rAEKthVbvAO3H-OvA-CQC573gL_C3B7EPGI7enztYZxjiM7-lXLAt8mOM74YlpTT8mi4HR-WASqOI75tknVlSXaIHvAVpPoavQg2VXhzmufo57ft7vL7-ubH1fXlxc3acUXndedd4Ay0Fl4wDaRjjgYSgtBBiDtteyLBEU3a2iKCB8aktlJ56zkPMvBz9OnoO5Xc4tTZDLE6SO23kA_VKM47rbRUjfz4X5LynlPBaAM_H0FXcq0FgplKHGxZDCXmsRkzTUYJQ01rptHvT7aHuwH8P_ZURQM-nABbnU2h9eBifeJ6xSRVsmHvjth9nXN5uu5a3mbE_wCSEKES</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13931521</pqid></control><display><type>article</type><title>Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels</title><source>JSTOR Archive Collection A-Z Listing</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>KAKEFUDA, G ; DUKE, S. H</creator><creatorcontrib>KAKEFUDA, G ; DUKE, S. H</creatorcontrib><description>An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, α-amylase, and β-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the transfer gel to its characteristic limit dextrin as it moves through the gel. Various limit dextrins in the starch gel are identified by their characteristic color development in KI/I solution. Isozymes of starch debranching enzyme, α-amylase, and β-amylase can be easily identified in the same gel.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.75.1.278</identifier><identifier>PMID: 16663595</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Alfalfa ; alpha -amylase ; Analytical, structural and metabolic biochemistry ; beta -amylase ; Biological and medical sciences ; Colors ; Dextrins ; Electrophoresis ; Enzymes ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Gels ; Hydrolases ; Hydrolysis ; Medicago sativa ; Nodules ; Short Communications ; Starches ; Sweet potatoes</subject><ispartof>Plant physiology (Bethesda), 1984-05, Vol.75 (1), p.278-280</ispartof><rights>Copyright 1984 The American Society of Plant Physiologists</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-4dcf32e885d528e042c1f0ff58f55b8a906ec0808f5131ede2268a67dad33f6f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4268663$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4268663$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9726176$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16663595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KAKEFUDA, G</creatorcontrib><creatorcontrib>DUKE, S. H</creatorcontrib><title>Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, α-amylase, and β-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the transfer gel to its characteristic limit dextrin as it moves through the gel. Various limit dextrins in the starch gel are identified by their characteristic color development in KI/I solution. Isozymes of starch debranching enzyme, α-amylase, and β-amylase can be easily identified in the same gel.</description><subject>Alfalfa</subject><subject>alpha -amylase</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>beta -amylase</subject><subject>Biological and medical sciences</subject><subject>Colors</subject><subject>Dextrins</subject><subject>Electrophoresis</subject><subject>Enzymes</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Hydrolases</subject><subject>Hydrolysis</subject><subject>Medicago sativa</subject><subject>Nodules</subject><subject>Short Communications</subject><subject>Starches</subject><subject>Sweet potatoes</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNp9kTtvFDEUhS0EIkugokXIBYIC7eLH-DFlFJYQKRIpltpy7GutI894sGeLScNfx2FXoaO6vsefj3x0EHpLyYZS0n2Zpo0SG7phSj9DKyo4WzPR6edoRUg7E637M_Sq1ntCCOW0e4nOqJSSi16s0O9tAjeXPO1zgTk6vCt2rAEKthVbvAO3H-OvA-CQC573gL_C3B7EPGI7enztYZxjiM7-lXLAt8mOM74YlpTT8mi4HR-WASqOI75tknVlSXaIHvAVpPoavQg2VXhzmufo57ft7vL7-ubH1fXlxc3acUXndedd4Ay0Fl4wDaRjjgYSgtBBiDtteyLBEU3a2iKCB8aktlJ56zkPMvBz9OnoO5Xc4tTZDLE6SO23kA_VKM47rbRUjfz4X5LynlPBaAM_H0FXcq0FgplKHGxZDCXmsRkzTUYJQ01rptHvT7aHuwH8P_ZURQM-nABbnU2h9eBifeJ6xSRVsmHvjth9nXN5uu5a3mbE_wCSEKES</recordid><startdate>198405</startdate><enddate>198405</enddate><creator>KAKEFUDA, G</creator><creator>DUKE, S. H</creator><general>American Society of Plant Physiologists</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>198405</creationdate><title>Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels</title><author>KAKEFUDA, G ; DUKE, S. H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-4dcf32e885d528e042c1f0ff58f55b8a906ec0808f5131ede2268a67dad33f6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Alfalfa</topic><topic>alpha -amylase</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>beta -amylase</topic><topic>Biological and medical sciences</topic><topic>Colors</topic><topic>Dextrins</topic><topic>Electrophoresis</topic><topic>Enzymes</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Hydrolases</topic><topic>Hydrolysis</topic><topic>Medicago sativa</topic><topic>Nodules</topic><topic>Short Communications</topic><topic>Starches</topic><topic>Sweet potatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KAKEFUDA, G</creatorcontrib><creatorcontrib>DUKE, S. H</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KAKEFUDA, G</au><au>DUKE, S. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1984-05</date><risdate>1984</risdate><volume>75</volume><issue>1</issue><spage>278</spage><epage>280</epage><pages>278-280</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, α-amylase, and β-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the transfer gel to its characteristic limit dextrin as it moves through the gel. Various limit dextrins in the starch gel are identified by their characteristic color development in KI/I solution. Isozymes of starch debranching enzyme, α-amylase, and β-amylase can be easily identified in the same gel.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16663595</pmid><doi>10.1104/pp.75.1.278</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1984-05, Vol.75 (1), p.278-280 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733487867 |
| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Alfalfa alpha -amylase Analytical, structural and metabolic biochemistry beta -amylase Biological and medical sciences Colors Dextrins Electrophoresis Enzymes Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Gels Hydrolases Hydrolysis Medicago sativa Nodules Short Communications Starches Sweet potatoes |
| title | Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T20%3A31%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrophoretic%20Transfer%20as%20a%20Technique%20for%20the%20Detection%20and%20Identification%20of%20Plant%20Amylolytic%20Enzymes%20in%20Polyacrylamide%20Gels&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=KAKEFUDA,%20G&rft.date=1984-05&rft.volume=75&rft.issue=1&rft.spage=278&rft.epage=280&rft.pages=278-280&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.75.1.278&rft_dat=%3Cjstor_proqu%3E4268663%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13931521&rft_id=info:pmid/16663595&rft_jstor_id=4268663&rfr_iscdi=true |