The Ligand for c-kit, Stem Cell Factor, Stimulates the Circulation of Cells That Engraft Lethally Irradiated Baboons
Recombinant human stem cell factor (SCF), the ligand for c-kit, has been shown to stimulate increased numbers of hematopoietic progenitor cells of multiple types to circulate in the blood of baboons, but it was not known if the cells stimulated to circulate by SCF contained cells capable of engrafti...
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Veröffentlicht in: | Blood 1992-12, Vol.80 (11), p.2715-2720 |
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creator | Andrews, Robert G. Bensinger, William I. Knitter, Glenn H. Bartelmez, Stephen H. Longin, Kevin Bernstein, Irwin D. Appelbaum, Frederick R. Zsebo, Krisztina M. |
description | Recombinant human stem cell factor (SCF), the ligand for c-kit, has been shown to stimulate increased numbers of hematopoietic progenitor cells of multiple types to circulate in the blood of baboons, but it was not known if the cells stimulated to circulate by SCF contained cells capable of engrafting and rescuing lethally irradiated baboons. Peripheral blood mononuclear cells (PBMNC) were collected by leukapheresis from four untreated control baboons and from three baboons on the 10th or 11th day of treatment with SCF (200 µg/kg/d). All animals were transplanted with 1.00 to 1.04 × 108/kg of cryopreserved autologous PBMNC after treatment with a single dose of 1,020 cGy total body irradiation (TBI). Three animals were transplanted with PBMNC that had been collected during SCF treatment, 24 to 38 days after the last dose of SCF. Rapid trilineage engraftment was documented by bone marrow biopsy in all three. The mean time to a total white blood cell count (WBC) ≥ 500/μL, WBC ≥ 1,000/μL, and an absolute neutrophil count (ANC) ≥ 500/μL was 15 ± 3 (mean ± SD), 19 ± 1, and 19 ± 2 days, respectively. Two animals remain alive with stable engraftment more than 180 and 245 days posttransplant. The third died of sepsis 32 days posttransplant with a hypercellular marrow showing trilineage engraftment. The surviving animals were transfusion independent by 10 and 59 days posttransplant. Four control animals were transplanted with PBMNC collected in the absence of SCF stimulation. One was treated for 11 days with SCF (200 μg/kg/d) after PBMNC were collected. This animal was transplanted 25 days after the last dose of SCF. None of the four control animals engrafted and they died 13, 16, 28, and 38 days posttransplant with marrow aplasia. Treatment with SCF stimulates the circulation of cells that engraft and rescue lethally irradiated baboons. The characteristics of the transplantable cells present in the circulation are now amenable to direct study. |
doi_str_mv | 10.1182/blood.V80.11.2715.2715 |
format | Article |
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Peripheral blood mononuclear cells (PBMNC) were collected by leukapheresis from four untreated control baboons and from three baboons on the 10th or 11th day of treatment with SCF (200 µg/kg/d). All animals were transplanted with 1.00 to 1.04 × 108/kg of cryopreserved autologous PBMNC after treatment with a single dose of 1,020 cGy total body irradiation (TBI). Three animals were transplanted with PBMNC that had been collected during SCF treatment, 24 to 38 days after the last dose of SCF. Rapid trilineage engraftment was documented by bone marrow biopsy in all three. The mean time to a total white blood cell count (WBC) ≥ 500/μL, WBC ≥ 1,000/μL, and an absolute neutrophil count (ANC) ≥ 500/μL was 15 ± 3 (mean ± SD), 19 ± 1, and 19 ± 2 days, respectively. Two animals remain alive with stable engraftment more than 180 and 245 days posttransplant. The third died of sepsis 32 days posttransplant with a hypercellular marrow showing trilineage engraftment. The surviving animals were transfusion independent by 10 and 59 days posttransplant. Four control animals were transplanted with PBMNC collected in the absence of SCF stimulation. One was treated for 11 days with SCF (200 μg/kg/d) after PBMNC were collected. This animal was transplanted 25 days after the last dose of SCF. None of the four control animals engrafted and they died 13, 16, 28, and 38 days posttransplant with marrow aplasia. Treatment with SCF stimulates the circulation of cells that engraft and rescue lethally irradiated baboons. The characteristics of the transplantable cells present in the circulation are now amenable to direct study.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V80.11.2715.2715</identifier><identifier>PMID: 1280476</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Biological and medical sciences ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Cryopreservation ; Female ; Hematopoietic Cell Growth Factors - pharmacology ; Leukapheresis ; Leukocyte Count - drug effects ; Male ; Medical sciences ; Monocytes - drug effects ; Monocytes - radiation effects ; Monocytes - transplantation ; Papio ; Platelet Count - drug effects ; Stem Cell Factor ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Transplantation, Autologous ; Whole-Body Irradiation</subject><ispartof>Blood, 1992-12, Vol.80 (11), p.2715-2720</ispartof><rights>1992 American Society of Hematology</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-9c889d2909c161d524b6561d6714994463a0b992f7d51d7d463fd7b375a6c24a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4507798$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1280476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andrews, Robert G.</creatorcontrib><creatorcontrib>Bensinger, William I.</creatorcontrib><creatorcontrib>Knitter, Glenn H.</creatorcontrib><creatorcontrib>Bartelmez, Stephen H.</creatorcontrib><creatorcontrib>Longin, Kevin</creatorcontrib><creatorcontrib>Bernstein, Irwin D.</creatorcontrib><creatorcontrib>Appelbaum, Frederick R.</creatorcontrib><creatorcontrib>Zsebo, Krisztina M.</creatorcontrib><title>The Ligand for c-kit, Stem Cell Factor, Stimulates the Circulation of Cells That Engraft Lethally Irradiated Baboons</title><title>Blood</title><addtitle>Blood</addtitle><description>Recombinant human stem cell factor (SCF), the ligand for c-kit, has been shown to stimulate increased numbers of hematopoietic progenitor cells of multiple types to circulate in the blood of baboons, but it was not known if the cells stimulated to circulate by SCF contained cells capable of engrafting and rescuing lethally irradiated baboons. Peripheral blood mononuclear cells (PBMNC) were collected by leukapheresis from four untreated control baboons and from three baboons on the 10th or 11th day of treatment with SCF (200 µg/kg/d). All animals were transplanted with 1.00 to 1.04 × 108/kg of cryopreserved autologous PBMNC after treatment with a single dose of 1,020 cGy total body irradiation (TBI). Three animals were transplanted with PBMNC that had been collected during SCF treatment, 24 to 38 days after the last dose of SCF. Rapid trilineage engraftment was documented by bone marrow biopsy in all three. The mean time to a total white blood cell count (WBC) ≥ 500/μL, WBC ≥ 1,000/μL, and an absolute neutrophil count (ANC) ≥ 500/μL was 15 ± 3 (mean ± SD), 19 ± 1, and 19 ± 2 days, respectively. Two animals remain alive with stable engraftment more than 180 and 245 days posttransplant. The third died of sepsis 32 days posttransplant with a hypercellular marrow showing trilineage engraftment. The surviving animals were transfusion independent by 10 and 59 days posttransplant. Four control animals were transplanted with PBMNC collected in the absence of SCF stimulation. One was treated for 11 days with SCF (200 μg/kg/d) after PBMNC were collected. This animal was transplanted 25 days after the last dose of SCF. None of the four control animals engrafted and they died 13, 16, 28, and 38 days posttransplant with marrow aplasia. Treatment with SCF stimulates the circulation of cells that engraft and rescue lethally irradiated baboons. The characteristics of the transplantable cells present in the circulation are now amenable to direct study.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Cryopreservation</subject><subject>Female</subject><subject>Hematopoietic Cell Growth Factors - pharmacology</subject><subject>Leukapheresis</subject><subject>Leukocyte Count - drug effects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - radiation effects</subject><subject>Monocytes - transplantation</subject><subject>Papio</subject><subject>Platelet Count - drug effects</subject><subject>Stem Cell Factor</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Transplantation, Autologous</subject><subject>Whole-Body Irradiation</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v3CAQxVHVKt2k_QitOFQ9xVvA2MAtySr_pJV66LZXNAacpbVNAmylfPvg3VVz7GXgzfwejB5CnylZUirZt24IwS5_yVkumaDNvrxBC9owWRHCyFu0IIS0FVeCvkenKf0mhPKaNSfohDJJuGgXKG-2Dq_9A0wW9yFiU_3x-Rz_yG7EKzcM-AZMDnHu-HE3QHYJ52JZ-Whm6cOEQ79HE95sIePr6SFCn_Ha5S0MwzO-jxGsL06Lr6ALYUof0LsehuQ-Hs8z9PPmerO6q9bfb-9Xl-vKcE5ypYyUyjJFlKEttQ3jXduUSysoV4rztgbSKcV6YRtqhS2N3oquFg20hnGoz9DXw7uPMTztXMp69MmUVWFyYZe0qGsuhaQFbA-giSGl6Hr9GP0I8VlToue49T5uXeIuUs9J70sxfjr-sOtGZ19th3zL_MtxDsnA0EeYjE__MN4QIZQs2MUBcyWNv95FnYx3k3HWR2eytsH_b5MX-O-d1Q</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Andrews, Robert G.</creator><creator>Bensinger, William I.</creator><creator>Knitter, Glenn H.</creator><creator>Bartelmez, Stephen H.</creator><creator>Longin, Kevin</creator><creator>Bernstein, Irwin D.</creator><creator>Appelbaum, Frederick R.</creator><creator>Zsebo, Krisztina M.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>The Ligand for c-kit, Stem Cell Factor, Stimulates the Circulation of Cells That Engraft Lethally Irradiated Baboons</title><author>Andrews, Robert G. ; Bensinger, William I. ; Knitter, Glenn H. ; Bartelmez, Stephen H. ; Longin, Kevin ; Bernstein, Irwin D. ; Appelbaum, Frederick R. ; Zsebo, Krisztina M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-9c889d2909c161d524b6561d6714994463a0b992f7d51d7d463fd7b375a6c24a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone marrow, stem cells transplantation. Graft versus host reaction</topic><topic>Cryopreservation</topic><topic>Female</topic><topic>Hematopoietic Cell Growth Factors - pharmacology</topic><topic>Leukapheresis</topic><topic>Leukocyte Count - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - radiation effects</topic><topic>Monocytes - transplantation</topic><topic>Papio</topic><topic>Platelet Count - drug effects</topic><topic>Stem Cell Factor</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><topic>Transplantation, Autologous</topic><topic>Whole-Body Irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andrews, Robert G.</creatorcontrib><creatorcontrib>Bensinger, William I.</creatorcontrib><creatorcontrib>Knitter, Glenn H.</creatorcontrib><creatorcontrib>Bartelmez, Stephen H.</creatorcontrib><creatorcontrib>Longin, Kevin</creatorcontrib><creatorcontrib>Bernstein, Irwin D.</creatorcontrib><creatorcontrib>Appelbaum, Frederick R.</creatorcontrib><creatorcontrib>Zsebo, Krisztina M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andrews, Robert G.</au><au>Bensinger, William I.</au><au>Knitter, Glenn H.</au><au>Bartelmez, Stephen H.</au><au>Longin, Kevin</au><au>Bernstein, Irwin D.</au><au>Appelbaum, Frederick R.</au><au>Zsebo, Krisztina M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Ligand for c-kit, Stem Cell Factor, Stimulates the Circulation of Cells That Engraft Lethally Irradiated Baboons</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>80</volume><issue>11</issue><spage>2715</spage><epage>2720</epage><pages>2715-2720</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Recombinant human stem cell factor (SCF), the ligand for c-kit, has been shown to stimulate increased numbers of hematopoietic progenitor cells of multiple types to circulate in the blood of baboons, but it was not known if the cells stimulated to circulate by SCF contained cells capable of engrafting and rescuing lethally irradiated baboons. Peripheral blood mononuclear cells (PBMNC) were collected by leukapheresis from four untreated control baboons and from three baboons on the 10th or 11th day of treatment with SCF (200 µg/kg/d). All animals were transplanted with 1.00 to 1.04 × 108/kg of cryopreserved autologous PBMNC after treatment with a single dose of 1,020 cGy total body irradiation (TBI). Three animals were transplanted with PBMNC that had been collected during SCF treatment, 24 to 38 days after the last dose of SCF. Rapid trilineage engraftment was documented by bone marrow biopsy in all three. The mean time to a total white blood cell count (WBC) ≥ 500/μL, WBC ≥ 1,000/μL, and an absolute neutrophil count (ANC) ≥ 500/μL was 15 ± 3 (mean ± SD), 19 ± 1, and 19 ± 2 days, respectively. Two animals remain alive with stable engraftment more than 180 and 245 days posttransplant. The third died of sepsis 32 days posttransplant with a hypercellular marrow showing trilineage engraftment. The surviving animals were transfusion independent by 10 and 59 days posttransplant. Four control animals were transplanted with PBMNC collected in the absence of SCF stimulation. One was treated for 11 days with SCF (200 μg/kg/d) after PBMNC were collected. This animal was transplanted 25 days after the last dose of SCF. None of the four control animals engrafted and they died 13, 16, 28, and 38 days posttransplant with marrow aplasia. Treatment with SCF stimulates the circulation of cells that engraft and rescue lethally irradiated baboons. The characteristics of the transplantable cells present in the circulation are now amenable to direct study.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>1280476</pmid><doi>10.1182/blood.V80.11.2715.2715</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Biological and medical sciences Bone marrow, stem cells transplantation. Graft versus host reaction Cryopreservation Female Hematopoietic Cell Growth Factors - pharmacology Leukapheresis Leukocyte Count - drug effects Male Medical sciences Monocytes - drug effects Monocytes - radiation effects Monocytes - transplantation Papio Platelet Count - drug effects Stem Cell Factor Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplantation, Autologous Whole-Body Irradiation |
title | The Ligand for c-kit, Stem Cell Factor, Stimulates the Circulation of Cells That Engraft Lethally Irradiated Baboons |
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