Effectiveness of Certain Sterilizing Agents for Seedling Incubation in Radiothymidine

Effectiveness of Certain Sterilizing Agents for Seedling Incubation in Radiothymidine

Failure of seed sterilization methods and breakdown in sterile technique can result in catabolism of thymidine by microorganisms during seedling incubation in radioisotope. The effectiveness of some sterilizing agents was monitored by microbiological methods and by thin layer chromatography for the...

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Veröffentlicht in:Plant physiology (Bethesda) 1969-02, Vol.44 (2), p.180-182
Hauptverfasser: McQuade, H. A., Alice A. Atchison, J. T. Parisi
Format: Artikel
Sprache:eng
Schlagworte:
Chemical sterilization
Germination
Hypochlorite
Microorganisms
Oxides
Peas
Seedlings
Sodium
Sterilization
Sunflower seeds
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container_title Plant physiology (Bethesda)
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creator McQuade, H. A.
Alice A. Atchison
J. T. Parisi
description Failure of seed sterilization methods and breakdown in sterile technique can result in catabolism of thymidine by microorganisms during seedling incubation in radioisotope. The effectiveness of some sterilizing agents was monitored by microbiological methods and by thin layer chromatography for the presence of thymidine degradation products. Sodium hypochlorite and ethylene oxide each sterilized pea and sunflower seeds. Sodium hypochlorite failed to sterilize onion seeds while ethylene oxide was only occasionally effective and often retarded germination. Captan, commonly used before germination to prevent fungal blight of seedlings, did not reduce the bacterial flora of onion seeds.
doi_str_mv 10.1104/pp.44.2.180
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source JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Chemical sterilization
Germination
Hypochlorite
Microorganisms
Oxides
Peas
Seedlings
Sodium
Sterilization
Sunflower seeds
title Effectiveness of Certain Sterilizing Agents for Seedling Incubation in Radiothymidine
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