Analysis of indole-3-acetic acid metabolites from Dalbergia dolichopetala by high performance liquid chromatography-mass spectrometry
A mixture of [2-14C1] and [13C6]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chroma...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1992-09, Vol.100 (1), p.63-68 |
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| creator | Ostin, A Monteiro, A.M Crozier, A Jensen, E Sandberg, G |
| description | A mixture of [2-14C1] and [13C6]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [14C1,13C6]indole-3-acetylaspartic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-14C1] or [13C6]indole-3-acetic acid into any of these conjugated indoles |
| doi_str_mv | 10.1104/pp.100.1.63 |
| format | Article |
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Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [14C1,13C6]indole-3-acetylaspartic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-14C1] or [13C6]indole-3-acetic acid into any of these conjugated indoles</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.100.1.63</identifier><identifier>PMID: 16653001</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Acetates ; ACIDE INDOLACETIQUE ; ACIDO INDOLACETICO ; Amino acid metabolism ; Biological and medical sciences ; CHROMATOGRAPHIE LIQUIDE HAUTE PRESS ; COTILEDONES ; COTYLEDON ; Cotyledons ; CROMATOGRAFIA LIQUIDA ALTA PRESION ; DALBERGIA ; Development and Growth Regulation ; ESPECTROMETRIA DE MASAS ; Fundamental and applied biological sciences. Psychology ; GRAINE ; Growth regulators ; INDOL ; INDOLES ; Liquids ; Mass spectra ; Mass spectrometers ; Metabolism ; METABOLISME ; METABOLISMO ; METABOLITE ; Metabolites ; METABOLITOS ; Plant physiology and development ; Plants ; Radioactive decay ; Seedlings ; SEMILLA ; SPECTROMETRIE DE MASSE</subject><ispartof>Plant physiology (Bethesda), 1992-09, Vol.100 (1), p.63-68</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-2ba21bcebb1fa22b26c8a563b038f549d9315731ca54e090d0191dd875afe8263</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4274593$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4274593$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4535300$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16653001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ostin, A</creatorcontrib><creatorcontrib>Monteiro, A.M</creatorcontrib><creatorcontrib>Crozier, A</creatorcontrib><creatorcontrib>Jensen, E</creatorcontrib><creatorcontrib>Sandberg, G</creatorcontrib><title>Analysis of indole-3-acetic acid metabolites from Dalbergia dolichopetala by high performance liquid chromatography-mass spectrometry</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A mixture of [2-14C1] and [13C6]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [14C1,13C6]indole-3-acetylaspartic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-14C1] or [13C6]indole-3-acetic acid into any of these conjugated indoles</description><subject>Acetates</subject><subject>ACIDE INDOLACETIQUE</subject><subject>ACIDO INDOLACETICO</subject><subject>Amino acid metabolism</subject><subject>Biological and medical sciences</subject><subject>CHROMATOGRAPHIE LIQUIDE HAUTE PRESS</subject><subject>COTILEDONES</subject><subject>COTYLEDON</subject><subject>Cotyledons</subject><subject>CROMATOGRAFIA LIQUIDA ALTA PRESION</subject><subject>DALBERGIA</subject><subject>Development and Growth Regulation</subject><subject>ESPECTROMETRIA DE MASAS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GRAINE</subject><subject>Growth regulators</subject><subject>INDOL</subject><subject>INDOLES</subject><subject>Liquids</subject><subject>Mass spectra</subject><subject>Mass spectrometers</subject><subject>Metabolism</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>METABOLITE</subject><subject>Metabolites</subject><subject>METABOLITOS</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Radioactive decay</subject><subject>Seedlings</subject><subject>SEMILLA</subject><subject>SPECTROMETRIE DE MASSE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpF0M1u3CAUBWBUtWomaVfdVVXFolIXladgwMbLKE1_pEhdtFlbFwxjIjwQYBZ-gL53GM2oWfmY-3GQLkLvKNlSSvjXGLeU1Lzt2Au0oYK1TSu4fIk2hNRMpBwu0GXOD4QQyih_jS5o1wlW_zbo3_Ue_JpdxsFit5-CNw1rQJviNAbtJryYAip4V0zGNoUFfwOvTNo5wFU7PYdYhQesVjy73YyjSTakBfbaYO8eD7VDz_UilLBLEOe1WSBnnKPRpR6bktY36JUFn83b8_cK3X-__Xvzs7n7_ePXzfVdo_nAStMqaKnSRilqoW1V22kJomOKMGkFH6aBUdEzqkFwQwYyETrQaZK9AGtk27Er9PnUG1N4PJhcxsVlbbyHvQmHPPaMcUk70lf55SR1CjknY8eY3AJpHSkZj2sfY6yx5rFjVX889x7UYqZne95zBZ_OALIGb1Pdjsv_HRfs6Cr7cGIPuYT0PG57LobjM-9PYwthhF2qDfd_hjqVgrInUFqePA</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Ostin, A</creator><creator>Monteiro, A.M</creator><creator>Crozier, A</creator><creator>Jensen, E</creator><creator>Sandberg, G</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920901</creationdate><title>Analysis of indole-3-acetic acid metabolites from Dalbergia dolichopetala by high performance liquid chromatography-mass spectrometry</title><author>Ostin, A ; Monteiro, A.M ; Crozier, A ; Jensen, E ; Sandberg, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-2ba21bcebb1fa22b26c8a563b038f549d9315731ca54e090d0191dd875afe8263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acetates</topic><topic>ACIDE INDOLACETIQUE</topic><topic>ACIDO INDOLACETICO</topic><topic>Amino acid metabolism</topic><topic>Biological and medical sciences</topic><topic>CHROMATOGRAPHIE LIQUIDE HAUTE PRESS</topic><topic>COTILEDONES</topic><topic>COTYLEDON</topic><topic>Cotyledons</topic><topic>CROMATOGRAFIA LIQUIDA ALTA PRESION</topic><topic>DALBERGIA</topic><topic>Development and Growth Regulation</topic><topic>ESPECTROMETRIA DE MASAS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GRAINE</topic><topic>Growth regulators</topic><topic>INDOL</topic><topic>INDOLES</topic><topic>Liquids</topic><topic>Mass spectra</topic><topic>Mass spectrometers</topic><topic>Metabolism</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>METABOLITE</topic><topic>Metabolites</topic><topic>METABOLITOS</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Radioactive decay</topic><topic>Seedlings</topic><topic>SEMILLA</topic><topic>SPECTROMETRIE DE MASSE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ostin, A</creatorcontrib><creatorcontrib>Monteiro, A.M</creatorcontrib><creatorcontrib>Crozier, A</creatorcontrib><creatorcontrib>Jensen, E</creatorcontrib><creatorcontrib>Sandberg, G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ostin, A</au><au>Monteiro, A.M</au><au>Crozier, A</au><au>Jensen, E</au><au>Sandberg, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of indole-3-acetic acid metabolites from Dalbergia dolichopetala by high performance liquid chromatography-mass spectrometry</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>100</volume><issue>1</issue><spage>63</spage><epage>68</epage><pages>63-68</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A mixture of [2-14C1] and [13C6]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [14C1,13C6]indole-3-acetylaspartic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-14C1] or [13C6]indole-3-acetic acid into any of these conjugated indoles</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16653001</pmid><doi>10.1104/pp.100.1.63</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1992-09, Vol.100 (1), p.63-68 |
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| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acetates ACIDE INDOLACETIQUE ACIDO INDOLACETICO Amino acid metabolism Biological and medical sciences CHROMATOGRAPHIE LIQUIDE HAUTE PRESS COTILEDONES COTYLEDON Cotyledons CROMATOGRAFIA LIQUIDA ALTA PRESION DALBERGIA Development and Growth Regulation ESPECTROMETRIA DE MASAS Fundamental and applied biological sciences. Psychology GRAINE Growth regulators INDOL INDOLES Liquids Mass spectra Mass spectrometers Metabolism METABOLISME METABOLISMO METABOLITE Metabolites METABOLITOS Plant physiology and development Plants Radioactive decay Seedlings SEMILLA SPECTROMETRIE DE MASSE |
| title | Analysis of indole-3-acetic acid metabolites from Dalbergia dolichopetala by high performance liquid chromatography-mass spectrometry |
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