Conjugation and radiolabeling of monoclonal antibodies with zirconium-89 for PET imaging using the bifunctional chelate p -isothiocyanatobenzyl-desferrioxamine

The positron emitter zirconium-89 ( 89 Zr) has very attractive properties for positron emission tomography (PET) imaging of intact monoclonal antibodies (mAbs) using immuno-PET. This protocol describes the step-by-step procedure for the facile radiolabeling of mAbs or other proteins with 89 Zr using...

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Veröffentlicht in:	Nature protocols 2010-04, Vol.5 (4), p.739-743
Hauptverfasser:	van Dongen, Guus A M S, Vosjan, Maria J W D, Perk, Lars R, Visser, Gerard W M, Budde, Marianne, Jurek, Paul, Kiefer, Garry E
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/245/2092 631/1647/664 Analytical Chemistry Animals Antibodies, Monoclonal - chemistry Biological Techniques Biomedical and Life Sciences Chelating Agents Computational Biology/Bioinformatics Deferoxamine - analogs & derivatives Emissions Filtration Head and Neck Neoplasms - diagnostic imaging Humans Isothiocyanates Life Sciences Methods Mice Mice, Nude Microarrays Monoclonal antibodies Organic Chemistry Oxalic acid PET imaging Positron-Emission Tomography - methods Properties Proteins Protocol Radioactive tracers Radioisotopes Radiopharmaceuticals - chemical synthesis Radiopharmaceuticals - chemistry Reagents Sodium Tomography Transplantation, Heterologous Zirconium
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