Diffusion of lactose in acidogenic biofilms
Effective diffusivity of lactose in active acidogenic biofilms was measured at 35 degrees C and pH 4.6 with a specially designed diffusion cell. The diffusion cell was designed and operated in such a way that the lactose concentrations on the surface and at the center of a living bacterial aggregate...
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| Veröffentlicht in: | Biotechnology and bioengineering 1993-03, Vol.41 (7), p.736-744 |
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| container_end_page | 744 |
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| container_title | Biotechnology and bioengineering |
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| creator | Yu, J. (University of British Columbia, Vancouver, BC, Canada) Pinder, K.L |
| description | Effective diffusivity of lactose in active acidogenic biofilms was measured at 35 degrees C and pH 4.6 with a specially designed diffusion cell. The diffusion cell was designed and operated in such a way that the lactose concentrations on the surface and at the center of a living bacterial aggregate could be measured at steady state. As a model parameter in a widely accepted reaction-diffusion equation which describes lactose distribution in living biofilms, the effective diffusivity of lactose in the biofilms was found to be about 65% of the lactose diffusivity in free solutions. It was experimentally determined that the active biofilms had about 66% void volume made up of channels through which the lactose molecules were transported into the bacterial aggregates. Therefore, the decrease in lactose diffusivity was mainly caused by the biofilm's solid biomass fraction rather than the tortuosity of the channels |
| doi_str_mv | 10.1002/bit.260410708 |
| format | Article |
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(University of British Columbia, Vancouver, BC, Canada) ; Pinder, K.L</creator><creatorcontrib>Yu, J. (University of British Columbia, Vancouver, BC, Canada) ; Pinder, K.L</creatorcontrib><description>Effective diffusivity of lactose in active acidogenic biofilms was measured at 35 degrees C and pH 4.6 with a specially designed diffusion cell. The diffusion cell was designed and operated in such a way that the lactose concentrations on the surface and at the center of a living bacterial aggregate could be measured at steady state. As a model parameter in a widely accepted reaction-diffusion equation which describes lactose distribution in living biofilms, the effective diffusivity of lactose in the biofilms was found to be about 65% of the lactose diffusivity in free solutions. It was experimentally determined that the active biofilms had about 66% void volume made up of channels through which the lactose molecules were transported into the bacterial aggregates. Therefore, the decrease in lactose diffusivity was mainly caused by the biofilm's solid biomass fraction rather than the tortuosity of the channels</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.260410708</identifier><identifier>PMID: 18609616</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>acidogenic biofilm ; ANAEROBIOSE ; ANAEROBIOSIS ; biofilm void fraction ; Biological and medical sciences ; Biotechnology ; DIFFUSION ; DIFUSION ; effective diffusivity ; FERMENTACION ; FERMENTATION ; Fundamental and applied biological sciences. Psychology ; LACTOSA ; LACTOSE ; LACTOSERUM ; Methods. Procedures. Technologies ; MICROORGANISME ; MICROORGANISMOS ; Others ; SUERO DE LA LECHE ; TRAITEMENT DES EAUX USEES ; TRATAMIENTO DE AGUAS RESIDUALES ; Various methods and equipments</subject><ispartof>Biotechnology and bioengineering, 1993-03, Vol.41 (7), p.736-744</ispartof><rights>Copyright © 1993 John Wiley & Sons, Inc.</rights><rights>1993 INIST-CNRS</rights><rights>(c) 1993 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5228-49f58c753177752c75565c05a021f2da41e3dfda27db3ca7073812287316d29c3</citedby><cites>FETCH-LOGICAL-c5228-49f58c753177752c75565c05a021f2da41e3dfda27db3ca7073812287316d29c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.260410708$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.260410708$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4661473$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18609616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, J. (University of British Columbia, Vancouver, BC, Canada)</creatorcontrib><creatorcontrib>Pinder, K.L</creatorcontrib><title>Diffusion of lactose in acidogenic biofilms</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Effective diffusivity of lactose in active acidogenic biofilms was measured at 35 degrees C and pH 4.6 with a specially designed diffusion cell. The diffusion cell was designed and operated in such a way that the lactose concentrations on the surface and at the center of a living bacterial aggregate could be measured at steady state. As a model parameter in a widely accepted reaction-diffusion equation which describes lactose distribution in living biofilms, the effective diffusivity of lactose in the biofilms was found to be about 65% of the lactose diffusivity in free solutions. It was experimentally determined that the active biofilms had about 66% void volume made up of channels through which the lactose molecules were transported into the bacterial aggregates. Therefore, the decrease in lactose diffusivity was mainly caused by the biofilm's solid biomass fraction rather than the tortuosity of the channels</description><subject>acidogenic biofilm</subject><subject>ANAEROBIOSE</subject><subject>ANAEROBIOSIS</subject><subject>biofilm void fraction</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>DIFFUSION</subject><subject>DIFUSION</subject><subject>effective diffusivity</subject><subject>FERMENTACION</subject><subject>FERMENTATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>LACTOSA</subject><subject>LACTOSE</subject><subject>LACTOSERUM</subject><subject>Methods. Procedures. Technologies</subject><subject>MICROORGANISME</subject><subject>MICROORGANISMOS</subject><subject>Others</subject><subject>SUERO DE LA LECHE</subject><subject>TRAITEMENT DES EAUX USEES</subject><subject>TRATAMIENTO DE AGUAS RESIDUALES</subject><subject>Various methods and equipments</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqF0M9PFDEUB_DGSGRdPXrhQOZA9EAGX3--6VFQfkk0BgiGS9PttKQ6O4XpboT_npKdrJ7w1Db5vNf3voS8o7BHAdjHWVzsMQWCAkLzgkwoaKyBaXhJJgCgai412ySvc_5Vntgo9Yps0kaBVlRNyO7nGMIyx9RXKVSddYuUfRX7yrrYphvfR1fNYgqxm-c3ZCPYLvu34zkll4dfLg6O67PvRycHn85qJxlraqGDbBxKThFRsnKTSjqQFhgNrLWCet6G1jJsZ9xZBOQNLYXIqWqZdnxKPqz63g7pbunzwsxjdr7rbO_TMhvkXCAr-xb5_llJlUCBiv0fctVorZ9gvYJuSDkPPpjbIc7t8GAomKfATQncrAMvfntsvJzNfftXjwkXsDMCm53twmB7F_PaCaWoKAtNCa7Yn9j5h-c_NfsnF_9OME4c88Lfryvt8Nso5CjN1bcjc01_Xu__-HpsTovfWvlgk7E3Qxnm8lwLkIwjfwQPzayY</recordid><startdate>19930325</startdate><enddate>19930325</enddate><creator>Yu, J. (University of British Columbia, Vancouver, BC, Canada)</creator><creator>Pinder, K.L</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QO</scope><scope>7TC</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19930325</creationdate><title>Diffusion of lactose in acidogenic biofilms</title><author>Yu, J. (University of British Columbia, Vancouver, BC, Canada) ; Pinder, K.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5228-49f58c753177752c75565c05a021f2da41e3dfda27db3ca7073812287316d29c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>acidogenic biofilm</topic><topic>ANAEROBIOSE</topic><topic>ANAEROBIOSIS</topic><topic>biofilm void fraction</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>DIFFUSION</topic><topic>DIFUSION</topic><topic>effective diffusivity</topic><topic>FERMENTACION</topic><topic>FERMENTATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>LACTOSA</topic><topic>LACTOSE</topic><topic>LACTOSERUM</topic><topic>Methods. Procedures. Technologies</topic><topic>MICROORGANISME</topic><topic>MICROORGANISMOS</topic><topic>Others</topic><topic>SUERO DE LA LECHE</topic><topic>TRAITEMENT DES EAUX USEES</topic><topic>TRATAMIENTO DE AGUAS RESIDUALES</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, J. 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(University of British Columbia, Vancouver, BC, Canada)</au><au>Pinder, K.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diffusion of lactose in acidogenic biofilms</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1993-03-25</date><risdate>1993</risdate><volume>41</volume><issue>7</issue><spage>736</spage><epage>744</epage><pages>736-744</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Effective diffusivity of lactose in active acidogenic biofilms was measured at 35 degrees C and pH 4.6 with a specially designed diffusion cell. The diffusion cell was designed and operated in such a way that the lactose concentrations on the surface and at the center of a living bacterial aggregate could be measured at steady state. As a model parameter in a widely accepted reaction-diffusion equation which describes lactose distribution in living biofilms, the effective diffusivity of lactose in the biofilms was found to be about 65% of the lactose diffusivity in free solutions. It was experimentally determined that the active biofilms had about 66% void volume made up of channels through which the lactose molecules were transported into the bacterial aggregates. Therefore, the decrease in lactose diffusivity was mainly caused by the biofilm's solid biomass fraction rather than the tortuosity of the channels</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18609616</pmid><doi>10.1002/bit.260410708</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biotechnology and bioengineering, 1993-03, Vol.41 (7), p.736-744 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | acidogenic biofilm ANAEROBIOSE ANAEROBIOSIS biofilm void fraction Biological and medical sciences Biotechnology DIFFUSION DIFUSION effective diffusivity FERMENTACION FERMENTATION Fundamental and applied biological sciences. Psychology LACTOSA LACTOSE LACTOSERUM Methods. Procedures. Technologies MICROORGANISME MICROORGANISMOS Others SUERO DE LA LECHE TRAITEMENT DES EAUX USEES TRATAMIENTO DE AGUAS RESIDUALES Various methods and equipments |
| title | Diffusion of lactose in acidogenic biofilms |
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