Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis
A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high‐voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulp...
Gespeichert in:
| Veröffentlicht in: | Phytochemical analysis 2003-05, Vol.14 (3), p.150-154 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 154 |
|---|---|
| container_issue | 3 |
| container_start_page | 150 |
| container_title | Phytochemical analysis |
| container_volume | 14 |
| creator | Asenstorfer, R.E Morgan, A.L Hayasaka, Y Sedgley, M Jones, G.P |
| description | A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high‐voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulphite‐addition compounds from crude extracts of plant tissue. In conjunction with liquid chromatography–electrospray mass spectrometry, the method enabled the anthocyanins from the flowers of two Banksia species and the leaves of two Acacia species to be identified. The Banksia flowers contained both cyanidin and peonidin‐based pigments, while the Acacia leaves contained cyanidin and delphinidin derivatives. Copyright © 2003 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/pca.696 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73346257</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73346257</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3756-ed2368dd28e89e869a4ffd900401cf0d0e49c89df63e72b3e108d9590b7f78273</originalsourceid><addsrcrecordid>eNp10MFu1DAQBmALUdGlIN4AcoIDShnbiRMftytoUatSiVYgLpbXGW9Ms3GwE2DfHq-yak-cZqT59EvzE_KKwikFYB8Go0-FFE_IgoKUOS1F-ZQsQJZ1DrwojsnzGH8CpJsUz8gxZZXkhaAL0t5MwVln9Oh8n3mb6X5svdnp3vUxs8FvszigcRj3xzPd30enE2qypdEmrVN0_SZr3abNf_tu1BvMBj1gyLBDMwY_tD5gdPEFObK6i_jyME_I3aePt6uL_OrL-efV8io3vCpFjg3jom4aVmMtsRZSF9Y2EqAAaiw0gIU0tWys4FixNUcKdSNLCevKVjWr-Al5O-cOwf-aMI5q66LBrtM9-imqiqfHWbmH72Zogo8xoFVDcFsddoqC2peqUqkqlZrk60PktN5i8-gOLSbwfgZ_XIe7_-Wom9Vyjstn7eKIfx-0DvdKVKkE9e36XH2n8vbHJb1Ql8m_mb3VXulNcFHdfWVAOVBgNeeM_wPUfZlR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73346257</pqid></control><display><type>article</type><title>Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Asenstorfer, R.E ; Morgan, A.L ; Hayasaka, Y ; Sedgley, M ; Jones, G.P</creator><creatorcontrib>Asenstorfer, R.E ; Morgan, A.L ; Hayasaka, Y ; Sedgley, M ; Jones, G.P</creatorcontrib><description>A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high‐voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulphite‐addition compounds from crude extracts of plant tissue. In conjunction with liquid chromatography–electrospray mass spectrometry, the method enabled the anthocyanins from the flowers of two Banksia species and the leaves of two Acacia species to be identified. The Banksia flowers contained both cyanidin and peonidin‐based pigments, while the Acacia leaves contained cyanidin and delphinidin derivatives. Copyright © 2003 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0958-0344</identifier><identifier>EISSN: 1099-1565</identifier><identifier>DOI: 10.1002/pca.696</identifier><identifier>PMID: 12793461</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Acacia ; Acacia - chemistry ; Acacia baileyana ; Acacia glaucoptera ; anthocyanin ; anthocyanins ; Anthocyanins - chemistry ; Anthocyanins - isolation & purification ; Banksia ; Banksia coccinea ; Banksia menziesii ; bisulfites ; bisulphite ; buffers ; chemical structure ; Chromatography, High Pressure Liquid ; cyanidin ; delphinidin ; Electrophoresis, Paper - methods ; floriculture crops ; flowers ; Flowers - chemistry ; high performance liquid chromatography ; high-voltage paper electrophoresis ; HPLC-MS ; leaves ; Molecular Structure ; new methods ; Paper electrophoresis ; peonidin ; phytochemicals ; plant extracts ; Plant Leaves - chemistry ; plant pigments ; Proteaceae - chemistry ; purification ; Spectrometry, Mass, Electrospray Ionization</subject><ispartof>Phytochemical analysis, 2003-05, Vol.14 (3), p.150-154</ispartof><rights>Copyright © 2003 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3756-ed2368dd28e89e869a4ffd900401cf0d0e49c89df63e72b3e108d9590b7f78273</citedby><cites>FETCH-LOGICAL-c3756-ed2368dd28e89e869a4ffd900401cf0d0e49c89df63e72b3e108d9590b7f78273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpca.696$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpca.696$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12793461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asenstorfer, R.E</creatorcontrib><creatorcontrib>Morgan, A.L</creatorcontrib><creatorcontrib>Hayasaka, Y</creatorcontrib><creatorcontrib>Sedgley, M</creatorcontrib><creatorcontrib>Jones, G.P</creatorcontrib><title>Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis</title><title>Phytochemical analysis</title><addtitle>Phytochem. Anal</addtitle><description>A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high‐voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulphite‐addition compounds from crude extracts of plant tissue. In conjunction with liquid chromatography–electrospray mass spectrometry, the method enabled the anthocyanins from the flowers of two Banksia species and the leaves of two Acacia species to be identified. The Banksia flowers contained both cyanidin and peonidin‐based pigments, while the Acacia leaves contained cyanidin and delphinidin derivatives. Copyright © 2003 John Wiley & Sons, Ltd.</description><subject>Acacia</subject><subject>Acacia - chemistry</subject><subject>Acacia baileyana</subject><subject>Acacia glaucoptera</subject><subject>anthocyanin</subject><subject>anthocyanins</subject><subject>Anthocyanins - chemistry</subject><subject>Anthocyanins - isolation & purification</subject><subject>Banksia</subject><subject>Banksia coccinea</subject><subject>Banksia menziesii</subject><subject>bisulfites</subject><subject>bisulphite</subject><subject>buffers</subject><subject>chemical structure</subject><subject>Chromatography, High Pressure Liquid</subject><subject>cyanidin</subject><subject>delphinidin</subject><subject>Electrophoresis, Paper - methods</subject><subject>floriculture crops</subject><subject>flowers</subject><subject>Flowers - chemistry</subject><subject>high performance liquid chromatography</subject><subject>high-voltage paper electrophoresis</subject><subject>HPLC-MS</subject><subject>leaves</subject><subject>Molecular Structure</subject><subject>new methods</subject><subject>Paper electrophoresis</subject><subject>peonidin</subject><subject>phytochemicals</subject><subject>plant extracts</subject><subject>Plant Leaves - chemistry</subject><subject>plant pigments</subject><subject>Proteaceae - chemistry</subject><subject>purification</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><issn>0958-0344</issn><issn>1099-1565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MFu1DAQBmALUdGlIN4AcoIDShnbiRMftytoUatSiVYgLpbXGW9Ms3GwE2DfHq-yak-cZqT59EvzE_KKwikFYB8Go0-FFE_IgoKUOS1F-ZQsQJZ1DrwojsnzGH8CpJsUz8gxZZXkhaAL0t5MwVln9Oh8n3mb6X5svdnp3vUxs8FvszigcRj3xzPd30enE2qypdEmrVN0_SZr3abNf_tu1BvMBj1gyLBDMwY_tD5gdPEFObK6i_jyME_I3aePt6uL_OrL-efV8io3vCpFjg3jom4aVmMtsRZSF9Y2EqAAaiw0gIU0tWys4FixNUcKdSNLCevKVjWr-Al5O-cOwf-aMI5q66LBrtM9-imqiqfHWbmH72Zogo8xoFVDcFsddoqC2peqUqkqlZrk60PktN5i8-gOLSbwfgZ_XIe7_-Wom9Vyjstn7eKIfx-0DvdKVKkE9e36XH2n8vbHJb1Ql8m_mb3VXulNcFHdfWVAOVBgNeeM_wPUfZlR</recordid><startdate>200305</startdate><enddate>200305</enddate><creator>Asenstorfer, R.E</creator><creator>Morgan, A.L</creator><creator>Hayasaka, Y</creator><creator>Sedgley, M</creator><creator>Jones, G.P</creator><general>John Wiley & Sons, Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200305</creationdate><title>Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis</title><author>Asenstorfer, R.E ; Morgan, A.L ; Hayasaka, Y ; Sedgley, M ; Jones, G.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3756-ed2368dd28e89e869a4ffd900401cf0d0e49c89df63e72b3e108d9590b7f78273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Acacia</topic><topic>Acacia - chemistry</topic><topic>Acacia baileyana</topic><topic>Acacia glaucoptera</topic><topic>anthocyanin</topic><topic>anthocyanins</topic><topic>Anthocyanins - chemistry</topic><topic>Anthocyanins - isolation & purification</topic><topic>Banksia</topic><topic>Banksia coccinea</topic><topic>Banksia menziesii</topic><topic>bisulfites</topic><topic>bisulphite</topic><topic>buffers</topic><topic>chemical structure</topic><topic>Chromatography, High Pressure Liquid</topic><topic>cyanidin</topic><topic>delphinidin</topic><topic>Electrophoresis, Paper - methods</topic><topic>floriculture crops</topic><topic>flowers</topic><topic>Flowers - chemistry</topic><topic>high performance liquid chromatography</topic><topic>high-voltage paper electrophoresis</topic><topic>HPLC-MS</topic><topic>leaves</topic><topic>Molecular Structure</topic><topic>new methods</topic><topic>Paper electrophoresis</topic><topic>peonidin</topic><topic>phytochemicals</topic><topic>plant extracts</topic><topic>Plant Leaves - chemistry</topic><topic>plant pigments</topic><topic>Proteaceae - chemistry</topic><topic>purification</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asenstorfer, R.E</creatorcontrib><creatorcontrib>Morgan, A.L</creatorcontrib><creatorcontrib>Hayasaka, Y</creatorcontrib><creatorcontrib>Sedgley, M</creatorcontrib><creatorcontrib>Jones, G.P</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asenstorfer, R.E</au><au>Morgan, A.L</au><au>Hayasaka, Y</au><au>Sedgley, M</au><au>Jones, G.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis</atitle><jtitle>Phytochemical analysis</jtitle><addtitle>Phytochem. Anal</addtitle><date>2003-05</date><risdate>2003</risdate><volume>14</volume><issue>3</issue><spage>150</spage><epage>154</epage><pages>150-154</pages><issn>0958-0344</issn><eissn>1099-1565</eissn><abstract>A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high‐voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulphite‐addition compounds from crude extracts of plant tissue. In conjunction with liquid chromatography–electrospray mass spectrometry, the method enabled the anthocyanins from the flowers of two Banksia species and the leaves of two Acacia species to be identified. The Banksia flowers contained both cyanidin and peonidin‐based pigments, while the Acacia leaves contained cyanidin and delphinidin derivatives. Copyright © 2003 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>12793461</pmid><doi>10.1002/pca.696</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0958-0344 |
| ispartof | Phytochemical analysis, 2003-05, Vol.14 (3), p.150-154 |
| issn | 0958-0344 1099-1565 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73346257 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Acacia Acacia - chemistry Acacia baileyana Acacia glaucoptera anthocyanin anthocyanins Anthocyanins - chemistry Anthocyanins - isolation & purification Banksia Banksia coccinea Banksia menziesii bisulfites bisulphite buffers chemical structure Chromatography, High Pressure Liquid cyanidin delphinidin Electrophoresis, Paper - methods floriculture crops flowers Flowers - chemistry high performance liquid chromatography high-voltage paper electrophoresis HPLC-MS leaves Molecular Structure new methods Paper electrophoresis peonidin phytochemicals plant extracts Plant Leaves - chemistry plant pigments Proteaceae - chemistry purification Spectrometry, Mass, Electrospray Ionization |
| title | Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T22%3A15%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20of%20anthocyanins%20from%20species%20of%20Banksia%20and%20Acacia%20using%20high-voltage%20paper%20electrophoresis&rft.jtitle=Phytochemical%20analysis&rft.au=Asenstorfer,%20R.E&rft.date=2003-05&rft.volume=14&rft.issue=3&rft.spage=150&rft.epage=154&rft.pages=150-154&rft.issn=0958-0344&rft.eissn=1099-1565&rft_id=info:doi/10.1002/pca.696&rft_dat=%3Cproquest_cross%3E73346257%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73346257&rft_id=info:pmid/12793461&rfr_iscdi=true |