High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver
High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of Arabidopsis thaliana. Single cell level spatial resolution of ∼12 μm was achieved by reducing the laser beam size by using an optical fibe...
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| Veröffentlicht in: | Analytical Chemistry 2010-04, Vol.82 (8), p.3255-3265 |
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| creator | Jun, Ji Hyun Song, Zhihong Liu, Zhenjiu Nikolau, Basil J Yeung, Edward S Lee, Young Jin |
| description | High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of Arabidopsis thaliana. Single cell level spatial resolution of ∼12 μm was achieved by reducing the laser beam size by using an optical fiber with 25 μm core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. The LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741) and C28 aldehyde (m/z 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces. |
| doi_str_mv | 10.1021/ac902990p |
| format | Article |
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Single cell level spatial resolution of ∼12 μm was achieved by reducing the laser beam size by using an optical fiber with 25 μm core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. The LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741) and C28 aldehyde (m/z 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac902990p</identifier><identifier>PMID: 20235569</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>ADDUCTS ; ALDEHYDES ; Aldehydes - chemistry ; ALKANES ; Alkanes - chemistry ; Analytical chemistry ; ARABIDOPSIS ; Arabidopsis - metabolism ; Chemistry ; Colloids - chemistry ; DESORPTION ; Exact sciences and technology ; FLOWERS ; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY ; ION SOURCES ; Ions ; LASERS ; LIPIDS ; MASS RESOLUTION ; MASS SPECTROMETERS ; Mass spectrometry ; MASS SPECTROSCOPY ; METABOLITES ; Molecules ; OPTICAL FIBERS ; OTHER INSTRUMENTATION ; Plant Roots - chemistry ; POLLEN ; RESOLUTION ; SILVER ; Silver - chemistry ; SILVER IONS ; SPATIAL RESOLUTION ; Spectrometric and optical methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; STAMEN</subject><ispartof>Analytical Chemistry, 2010-04, Vol.82 (8), p.3255-3265</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Apr 15, 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a498t-f472aa4cfe5ad79fa815be8f7db1c5ee1f1cbe58d9385264b03278cfc00bcdd53</citedby><cites>FETCH-LOGICAL-a498t-f472aa4cfe5ad79fa815be8f7db1c5ee1f1cbe58d9385264b03278cfc00bcdd53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac902990p$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac902990p$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,881,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22628098$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20235569$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/976957$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Jun, Ji Hyun</creatorcontrib><creatorcontrib>Song, Zhihong</creatorcontrib><creatorcontrib>Liu, Zhenjiu</creatorcontrib><creatorcontrib>Nikolau, Basil J</creatorcontrib><creatorcontrib>Yeung, Edward S</creatorcontrib><creatorcontrib>Lee, Young Jin</creatorcontrib><creatorcontrib>Ames Laboratory (AMES), Ames, IA (United States)</creatorcontrib><title>High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver</title><title>Analytical Chemistry</title><addtitle>Anal. Chem</addtitle><description>High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of Arabidopsis thaliana. Single cell level spatial resolution of ∼12 μm was achieved by reducing the laser beam size by using an optical fiber with 25 μm core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. The LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741) and C28 aldehyde (m/z 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces.</description><subject>ADDUCTS</subject><subject>ALDEHYDES</subject><subject>Aldehydes - chemistry</subject><subject>ALKANES</subject><subject>Alkanes - chemistry</subject><subject>Analytical chemistry</subject><subject>ARABIDOPSIS</subject><subject>Arabidopsis - metabolism</subject><subject>Chemistry</subject><subject>Colloids - chemistry</subject><subject>DESORPTION</subject><subject>Exact sciences and technology</subject><subject>FLOWERS</subject><subject>INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY</subject><subject>ION SOURCES</subject><subject>Ions</subject><subject>LASERS</subject><subject>LIPIDS</subject><subject>MASS RESOLUTION</subject><subject>MASS SPECTROMETERS</subject><subject>Mass spectrometry</subject><subject>MASS SPECTROSCOPY</subject><subject>METABOLITES</subject><subject>Molecules</subject><subject>OPTICAL FIBERS</subject><subject>OTHER INSTRUMENTATION</subject><subject>Plant Roots - chemistry</subject><subject>POLLEN</subject><subject>RESOLUTION</subject><subject>SILVER</subject><subject>Silver - chemistry</subject><subject>SILVER IONS</subject><subject>SPATIAL RESOLUTION</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>STAMEN</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0V2L1DAUBuAiijuuXvgHJAoiXlSTtGnSy2X82IFZBMe9LqdpMpMl03RzWmH8Pf5QMx_ugF4FDg_vOeHNspeMfmCUs4-ga8rrmg6PshkTnOaVUvxxNqOUFjmXlF5kzxDvKGWMsuppdsEpL4So6ln2-9qtN_lqgNGBJ9B35DC4AUTy3WDw0-hCTxZbWLt-TYIlqyla0IbcmBHa4N1ocD--itC6LgzokIwb8A56IO2OLAFNJJ9SVBz2Ufki9O4XHFIPW1aD0WMMWzPGHbnF_ZZ58D64Lh20cv6nic-zJxY8mhen9zK7_fL5x_w6X377uphfLXMoazXmtpQcoNTWCOhkbUEx0RplZdcyLYxhlunWCNXVhRK8KltacKm01ZS2uutEcZm9PuYGHF2DOv1Nb3To-3RhU8uqFjKZd0czxHA_GRybrUNtvIfehAkbWRSlkKwqknzzj7wLU-zTBxrOZGqoKquE3h-RjgExGtsM0W0h7hpGm325zUO5yb46BU7t1nQP8m-bCbw9AUAN3kbotcOz4xVXtFZnBxrPR_2_8A9TkrrM</recordid><startdate>20100415</startdate><enddate>20100415</enddate><creator>Jun, Ji Hyun</creator><creator>Song, Zhihong</creator><creator>Liu, Zhenjiu</creator><creator>Nikolau, Basil J</creator><creator>Yeung, Edward S</creator><creator>Lee, Young Jin</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20100415</creationdate><title>High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver</title><author>Jun, Ji Hyun ; Song, Zhihong ; Liu, Zhenjiu ; Nikolau, Basil J ; Yeung, Edward S ; Lee, Young Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a498t-f472aa4cfe5ad79fa815be8f7db1c5ee1f1cbe58d9385264b03278cfc00bcdd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>ADDUCTS</topic><topic>ALDEHYDES</topic><topic>Aldehydes - chemistry</topic><topic>ALKANES</topic><topic>Alkanes - chemistry</topic><topic>Analytical chemistry</topic><topic>ARABIDOPSIS</topic><topic>Arabidopsis - metabolism</topic><topic>Chemistry</topic><topic>Colloids - chemistry</topic><topic>DESORPTION</topic><topic>Exact sciences and technology</topic><topic>FLOWERS</topic><topic>INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY</topic><topic>ION SOURCES</topic><topic>Ions</topic><topic>LASERS</topic><topic>LIPIDS</topic><topic>MASS RESOLUTION</topic><topic>MASS SPECTROMETERS</topic><topic>Mass spectrometry</topic><topic>MASS SPECTROSCOPY</topic><topic>METABOLITES</topic><topic>Molecules</topic><topic>OPTICAL FIBERS</topic><topic>OTHER INSTRUMENTATION</topic><topic>Plant Roots - chemistry</topic><topic>POLLEN</topic><topic>RESOLUTION</topic><topic>SILVER</topic><topic>Silver - chemistry</topic><topic>SILVER IONS</topic><topic>SPATIAL RESOLUTION</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>STAMEN</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jun, Ji Hyun</creatorcontrib><creatorcontrib>Song, Zhihong</creatorcontrib><creatorcontrib>Liu, Zhenjiu</creatorcontrib><creatorcontrib>Nikolau, Basil J</creatorcontrib><creatorcontrib>Yeung, Edward S</creatorcontrib><creatorcontrib>Lee, Young Jin</creatorcontrib><creatorcontrib>Ames Laboratory (AMES), Ames, IA (United States)</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Analytical Chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jun, Ji Hyun</au><au>Song, Zhihong</au><au>Liu, Zhenjiu</au><au>Nikolau, Basil J</au><au>Yeung, Edward S</au><au>Lee, Young Jin</au><aucorp>Ames Laboratory (AMES), Ames, IA (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver</atitle><jtitle>Analytical Chemistry</jtitle><addtitle>Anal. Chem</addtitle><date>2010-04-15</date><risdate>2010</risdate><volume>82</volume><issue>8</issue><spage>3255</spage><epage>3265</epage><pages>3255-3265</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of Arabidopsis thaliana. Single cell level spatial resolution of ∼12 μm was achieved by reducing the laser beam size by using an optical fiber with 25 μm core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. The LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741) and C28 aldehyde (m/z 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20235569</pmid><doi>10.1021/ac902990p</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | ADDUCTS ALDEHYDES Aldehydes - chemistry ALKANES Alkanes - chemistry Analytical chemistry ARABIDOPSIS Arabidopsis - metabolism Chemistry Colloids - chemistry DESORPTION Exact sciences and technology FLOWERS INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY ION SOURCES Ions LASERS LIPIDS MASS RESOLUTION MASS SPECTROMETERS Mass spectrometry MASS SPECTROSCOPY METABOLITES Molecules OPTICAL FIBERS OTHER INSTRUMENTATION Plant Roots - chemistry POLLEN RESOLUTION SILVER Silver - chemistry SILVER IONS SPATIAL RESOLUTION Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods STAMEN |
| title | High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver |
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