Initial characterization of a reductive dehalogenase from Desulfitobacterium chlororespirans Co23
Desulfitobacterium chlororespirans Co23 is capable of using 3-chloro-4-hydroxybenzoate as terminal electron acceptor for growth. Membrane preparations from cells grown fermentatively on pyruvate in the presence of 3-chloro-4-hydroxybenzoate dechlorinated this compound at a rate of 3.9 nmol min-1 mg...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1996-10, Vol.62 (10), p.3809-3813 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Loffler, F.E. (Michigan State University, East Lansing, MI.) Sanford, R.A Tiedje, J.M |
| description | Desulfitobacterium chlororespirans Co23 is capable of using 3-chloro-4-hydroxybenzoate as terminal electron acceptor for growth. Membrane preparations from cells grown fermentatively on pyruvate in the presence of 3-chloro-4-hydroxybenzoate dechlorinated this compound at a rate of 3.9 nmol min-1 mg of protein-1. Fivefold-greater dechlorination rates were measured with reduced methyl viologen as the artificial electron donor. Reduced benzyl viologen, NADH, NADPH, reduced flavin adenine dinucleotide, and reduced flavin mononucleotide could not substitute for reduced methyl viologen. The maximal initial rate of catalysis was achieved at pH 6.5 and 60 degrees C. The membrane-bound dechlorinating enzyme system was not oxygen sensitive and was stable at 57 degrees C for at least 2 h. Sulfite inhibited dechlorination in cell-free assays, whereas sulfate did not. Several chlorophenols were dehalogenated exclusively in the ortho position by cell extracts |
| doi_str_mv | 10.1128/aem.62.10.3809-3813.1996 |
| format | Article |
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(Michigan State University, East Lansing, MI.) ; Sanford, R.A ; Tiedje, J.M</creator><creatorcontrib>Loffler, F.E. (Michigan State University, East Lansing, MI.) ; Sanford, R.A ; Tiedje, J.M</creatorcontrib><description>Desulfitobacterium chlororespirans Co23 is capable of using 3-chloro-4-hydroxybenzoate as terminal electron acceptor for growth. Membrane preparations from cells grown fermentatively on pyruvate in the presence of 3-chloro-4-hydroxybenzoate dechlorinated this compound at a rate of 3.9 nmol min-1 mg of protein-1. Fivefold-greater dechlorination rates were measured with reduced methyl viologen as the artificial electron donor. Reduced benzyl viologen, NADH, NADPH, reduced flavin adenine dinucleotide, and reduced flavin mononucleotide could not substitute for reduced methyl viologen. The maximal initial rate of catalysis was achieved at pH 6.5 and 60 degrees C. The membrane-bound dechlorinating enzyme system was not oxygen sensitive and was stable at 57 degrees C for at least 2 h. Sulfite inhibited dechlorination in cell-free assays, whereas sulfate did not. Several chlorophenols were dehalogenated exclusively in the ortho position by cell extracts</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.62.10.3809-3813.1996</identifier><identifier>PMID: 16535425</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ACIDE BENZOIQUE ; ACIDO BENZOICO ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ANAEROBIOSE ; ANAEROBIOSIS ; BACTERIA ; BACTERIA GRAM POSITIVA ; BACTERIE GRAM POSITIF ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; CHLORE ; CLORO ; COMPOSE ORGANOCHLORE ; COMPUESTO ORGANICO DEL CLORO ; Desulfitobacterium chlororespirans ; Enzymes ; FLORA DEL SUELO ; FLORE DU SOL ; Fundamental and applied biological sciences. Psychology ; Microbiology ; MICROORGANISME ; MICROORGANISMOS ; Microorganisms ; Mission oriented research ; Oxidation ; OXIDORREDUCTASAS ; OXIGENO ; OXIRREDUCION ; OXYDOREDUCTASE ; OXYDOREDUCTION ; OXYGENE ; Physiology and metabolism ; REACCIONES QUIMICAS ; REACTION CHIMIQUE ; REDUCCION ; REDUCTION ; TEMPERATURA ; TEMPERATURE</subject><ispartof>Applied and Environmental Microbiology, 1996-10, Vol.62 (10), p.3809-3813</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Oct 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c671t-d1962da62433fc45de89c00ebb8ee48b41b11203ba31db6e629f1ef94b0a2a93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388963/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388963/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,3190,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2474655$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16535425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loffler, F.E. (Michigan State University, East Lansing, MI.)</creatorcontrib><creatorcontrib>Sanford, R.A</creatorcontrib><creatorcontrib>Tiedje, J.M</creatorcontrib><title>Initial characterization of a reductive dehalogenase from Desulfitobacterium chlororespirans Co23</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Desulfitobacterium chlororespirans Co23 is capable of using 3-chloro-4-hydroxybenzoate as terminal electron acceptor for growth. Membrane preparations from cells grown fermentatively on pyruvate in the presence of 3-chloro-4-hydroxybenzoate dechlorinated this compound at a rate of 3.9 nmol min-1 mg of protein-1. Fivefold-greater dechlorination rates were measured with reduced methyl viologen as the artificial electron donor. Reduced benzyl viologen, NADH, NADPH, reduced flavin adenine dinucleotide, and reduced flavin mononucleotide could not substitute for reduced methyl viologen. The maximal initial rate of catalysis was achieved at pH 6.5 and 60 degrees C. The membrane-bound dechlorinating enzyme system was not oxygen sensitive and was stable at 57 degrees C for at least 2 h. Sulfite inhibited dechlorination in cell-free assays, whereas sulfate did not. Several chlorophenols were dehalogenated exclusively in the ortho position by cell extracts</description><subject>ACIDE BENZOIQUE</subject><subject>ACIDO BENZOICO</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ANAEROBIOSE</subject><subject>ANAEROBIOSIS</subject><subject>BACTERIA</subject><subject>BACTERIA GRAM POSITIVA</subject><subject>BACTERIE GRAM POSITIF</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>CHLORE</subject><subject>CLORO</subject><subject>COMPOSE ORGANOCHLORE</subject><subject>COMPUESTO ORGANICO DEL CLORO</subject><subject>Desulfitobacterium chlororespirans</subject><subject>Enzymes</subject><subject>FLORA DEL SUELO</subject><subject>FLORE DU SOL</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Microbiology</topic><topic>MICROORGANISME</topic><topic>MICROORGANISMOS</topic><topic>Microorganisms</topic><topic>Mission oriented research</topic><topic>Oxidation</topic><topic>OXIDORREDUCTASAS</topic><topic>OXIGENO</topic><topic>OXIRREDUCION</topic><topic>OXYDOREDUCTASE</topic><topic>OXYDOREDUCTION</topic><topic>OXYGENE</topic><topic>Physiology and metabolism</topic><topic>REACCIONES QUIMICAS</topic><topic>REACTION CHIMIQUE</topic><topic>REDUCCION</topic><topic>REDUCTION</topic><topic>TEMPERATURA</topic><topic>TEMPERATURE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loffler, F.E. 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(Michigan State University, East Lansing, MI.)</au><au>Sanford, R.A</au><au>Tiedje, J.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Initial characterization of a reductive dehalogenase from Desulfitobacterium chlororespirans Co23</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1996-10-01</date><risdate>1996</risdate><volume>62</volume><issue>10</issue><spage>3809</spage><epage>3813</epage><pages>3809-3813</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Desulfitobacterium chlororespirans Co23 is capable of using 3-chloro-4-hydroxybenzoate as terminal electron acceptor for growth. Membrane preparations from cells grown fermentatively on pyruvate in the presence of 3-chloro-4-hydroxybenzoate dechlorinated this compound at a rate of 3.9 nmol min-1 mg of protein-1. Fivefold-greater dechlorination rates were measured with reduced methyl viologen as the artificial electron donor. Reduced benzyl viologen, NADH, NADPH, reduced flavin adenine dinucleotide, and reduced flavin mononucleotide could not substitute for reduced methyl viologen. The maximal initial rate of catalysis was achieved at pH 6.5 and 60 degrees C. The membrane-bound dechlorinating enzyme system was not oxygen sensitive and was stable at 57 degrees C for at least 2 h. Sulfite inhibited dechlorination in cell-free assays, whereas sulfate did not. Several chlorophenols were dehalogenated exclusively in the ortho position by cell extracts</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16535425</pmid><doi>10.1128/aem.62.10.3809-3813.1996</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 1996-10, Vol.62 (10), p.3809-3813 |
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| language | eng |
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| source | ASM_美国微生物学会期刊; PubMed Central; Alma/SFX Local Collection |
| subjects | ACIDE BENZOIQUE ACIDO BENZOICO ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ANAEROBIOSE ANAEROBIOSIS BACTERIA BACTERIA GRAM POSITIVA BACTERIE GRAM POSITIF Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology CHLORE CLORO COMPOSE ORGANOCHLORE COMPUESTO ORGANICO DEL CLORO Desulfitobacterium chlororespirans Enzymes FLORA DEL SUELO FLORE DU SOL Fundamental and applied biological sciences. Psychology Microbiology MICROORGANISME MICROORGANISMOS Microorganisms Mission oriented research Oxidation OXIDORREDUCTASAS OXIGENO OXIRREDUCION OXYDOREDUCTASE OXYDOREDUCTION OXYGENE Physiology and metabolism REACCIONES QUIMICAS REACTION CHIMIQUE REDUCCION REDUCTION TEMPERATURA TEMPERATURE |
| title | Initial characterization of a reductive dehalogenase from Desulfitobacterium chlororespirans Co23 |
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