Genetically engineered Erwinia carotovora: survival, intraspecific competition, and effects upon selected bacterial genera
Environmental use of genetically engineered microorganisms has raised concerns about potential ecological impact. This research evaluated the survival, competitiveness, and effects upon selected bacterial genera of wild-type and genetically engineered Erwinia carotovora subsp. carotovora to ascertai...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1990-06, Vol.56 (6), p.1689-1694 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Orvos, D.R. (Roy F. Weston, Inc., West Chester, PA) Lacy, G.H Cairns, J. Jr |
| description | Environmental use of genetically engineered microorganisms has raised concerns about potential ecological impact. This research evaluated the survival, competitiveness, and effects upon selected bacterial genera of wild-type and genetically engineered Erwinia carotovora subsp. carotovora to ascertain if differences between the wild-type and genetically engineered strains exist in soil microcosms. The engineered strain contained a chromosomally inserted gene for kanamycin resistance. No significant differences in survival in nonsterile soil over 2 months or in the competitiveness of either strain were observed when the strains were added concurrently to microcosms. For reasons that remain unclear, the engineered strain did survive longer in sterilized soil. The effects of both strains on total bacteria, Pseudomonas and Staphylococcus strains, and actinomycetes were observed. While some apparent differences were observed, they were not statistically significant. A better understanding of the microbial ecology of engineered bacteria, especially pathogens genetically altered for use as biological control agents, is essential before commercial applications can be accomplished |
| doi_str_mv | 10.1128/AEM.56.6.1689-1694.1990 |
| format | Article |
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(Roy F. Weston, Inc., West Chester, PA) ; Lacy, G.H ; Cairns, J. Jr</creator><creatorcontrib>Orvos, D.R. (Roy F. Weston, Inc., West Chester, PA) ; Lacy, G.H ; Cairns, J. Jr</creatorcontrib><description>Environmental use of genetically engineered microorganisms has raised concerns about potential ecological impact. This research evaluated the survival, competitiveness, and effects upon selected bacterial genera of wild-type and genetically engineered Erwinia carotovora subsp. carotovora to ascertain if differences between the wild-type and genetically engineered strains exist in soil microcosms. The engineered strain contained a chromosomally inserted gene for kanamycin resistance. No significant differences in survival in nonsterile soil over 2 months or in the competitiveness of either strain were observed when the strains were added concurrently to microcosms. For reasons that remain unclear, the engineered strain did survive longer in sterilized soil. The effects of both strains on total bacteria, Pseudomonas and Staphylococcus strains, and actinomycetes were observed. While some apparent differences were observed, they were not statistically significant. A better understanding of the microbial ecology of engineered bacteria, especially pathogens genetically altered for use as biological control agents, is essential before commercial applications can be accomplished</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.56.6.1689-1694.1990</identifier><identifier>PMID: 16348212</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>antibiotic resistance ; Biological and medical sciences ; Biotechnology ; Environment and pollution ; ERWINIA CAROTOVORA ; Fundamental and applied biological sciences. Psychology ; genes ; genetic engineering ; Genetically engineered organisms behavior (microorganisms, plants, animals) ; Genetics and Molecular Biology ; GENIE GENETIQUE ; Industrial applications and implications. 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(Roy F. Weston, Inc., West Chester, PA)</creatorcontrib><creatorcontrib>Lacy, G.H</creatorcontrib><creatorcontrib>Cairns, J. Jr</creatorcontrib><title>Genetically engineered Erwinia carotovora: survival, intraspecific competition, and effects upon selected bacterial genera</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Environmental use of genetically engineered microorganisms has raised concerns about potential ecological impact. This research evaluated the survival, competitiveness, and effects upon selected bacterial genera of wild-type and genetically engineered Erwinia carotovora subsp. carotovora to ascertain if differences between the wild-type and genetically engineered strains exist in soil microcosms. The engineered strain contained a chromosomally inserted gene for kanamycin resistance. No significant differences in survival in nonsterile soil over 2 months or in the competitiveness of either strain were observed when the strains were added concurrently to microcosms. For reasons that remain unclear, the engineered strain did survive longer in sterilized soil. The effects of both strains on total bacteria, Pseudomonas and Staphylococcus strains, and actinomycetes were observed. While some apparent differences were observed, they were not statistically significant. A better understanding of the microbial ecology of engineered bacteria, especially pathogens genetically altered for use as biological control agents, is essential before commercial applications can be accomplished</description><subject>antibiotic resistance</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Environment and pollution</subject><subject>ERWINIA CAROTOVORA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>genetic engineering</subject><subject>Genetically engineered organisms behavior (microorganisms, plants, animals)</subject><subject>Genetics and Molecular Biology</subject><subject>GENIE GENETIQUE</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>INGENIERIA GENETICA</subject><subject>Pseudomonas</subject><subject>Staphylococcus</subject><subject>subsp. carotovora</subject><subject>SUPERVIVENCIA</subject><subject>SURVIE</subject><subject>survival</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp9kUFv1DAQhSMEokvhD3AAcwAuzWI7jmMjcaiqpSAVcYCerYkz2TVK7K2d3ar8ehztitILp5Hl7703o1cUrxldMsbVh_PVt2Utl3LJpNIlk1osmdb0UbFgVKuyrir5uFhQqnXJuaAnxbOUflFKBZXqaXHCZCUUZ3xR_L5Ej5OzMAx3BP3aecSIHVnFW-cdEAsxTGEfInwkaRf3bg_DGXF-ipC2aF3vLLFh3GaPyQV_RsB3BPse7ZTIbhs8STjkR7ZsIY_oYCDrnBnhefGkhyHhi-M8La4_r35efCmvvl9-vTi_Kq2keio1E62QqNvWMmga2nVUYcdBiV52nebALfbItWpraxm1Dcvpqq50Z3krsapOi08H3-2uHbGzOC8_mG10I8Q7E8CZhz_ebcw67A1TQmiR9e-P-hhudpgmM7pkcRjAY9gl01SVEFJxnsl3_yWZ5I2Uos5gcwBtDClF7P9uw6iZCzaAo6mlkWYu2MwFm7ngrHz17zH3umOjGXh7BCDlVvsI3rp0z2nJK0Zn7s2B27j15tZFNJDGh7GZeXlgeggG1jH7XP_QVNQ6H_sHsNXHTg</recordid><startdate>19900601</startdate><enddate>19900601</enddate><creator>Orvos, D.R. 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Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetically engineered Erwinia carotovora: survival, intraspecific competition, and effects upon selected bacterial genera</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1990-06-01</date><risdate>1990</risdate><volume>56</volume><issue>6</issue><spage>1689</spage><epage>1694</epage><pages>1689-1694</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Environmental use of genetically engineered microorganisms has raised concerns about potential ecological impact. This research evaluated the survival, competitiveness, and effects upon selected bacterial genera of wild-type and genetically engineered Erwinia carotovora subsp. carotovora to ascertain if differences between the wild-type and genetically engineered strains exist in soil microcosms. The engineered strain contained a chromosomally inserted gene for kanamycin resistance. No significant differences in survival in nonsterile soil over 2 months or in the competitiveness of either strain were observed when the strains were added concurrently to microcosms. For reasons that remain unclear, the engineered strain did survive longer in sterilized soil. The effects of both strains on total bacteria, Pseudomonas and Staphylococcus strains, and actinomycetes were observed. While some apparent differences were observed, they were not statistically significant. A better understanding of the microbial ecology of engineered bacteria, especially pathogens genetically altered for use as biological control agents, is essential before commercial applications can be accomplished</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16348212</pmid><doi>10.1128/AEM.56.6.1689-1694.1990</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; PubMed Central; Alma/SFX Local Collection |
| subjects | antibiotic resistance Biological and medical sciences Biotechnology Environment and pollution ERWINIA CAROTOVORA Fundamental and applied biological sciences. Psychology genes genetic engineering Genetically engineered organisms behavior (microorganisms, plants, animals) Genetics and Molecular Biology GENIE GENETIQUE Industrial applications and implications. Economical aspects INGENIERIA GENETICA Pseudomonas Staphylococcus subsp. carotovora SUPERVIVENCIA SURVIE survival |
| title | Genetically engineered Erwinia carotovora: survival, intraspecific competition, and effects upon selected bacterial genera |
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