Inhibition of osteopontin reduces liver metastasis of human pancreatic cancer xenografts injected into the spleen in a mouse model
Purpose Pancreatic cancer is associated with the poorest prognosis of any digestive cancer due to the high incidence of liver metastasis. This study evaluated the possibility that osteopontin (OPN) RNA interference (RNAi) and anti-OPN antibody (Ab) could have antimetastatic effects. Methods The diff...
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| Veröffentlicht in: | Surgery today (Tokyo, Japan) Japan), 2010-04, Vol.40 (4), p.347-356 |
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| creator | Ohno, Keisuke Nishimori, Hidefumi Yasoshima, Takahiro Kamiguchi, Kenjiro Hata, Fumitake Fukui, Rika Okuya, Koichi Kimura, Yasutoshi Denno, Ryuichi Kon, Shigeyuki Uede, Toshimitsu Sato, Noriyuki Hirata, Koichi |
| description | Purpose
Pancreatic cancer is associated with the poorest prognosis of any digestive cancer due to the high incidence of liver metastasis. This study evaluated the possibility that osteopontin (OPN) RNA interference (RNAi) and anti-OPN antibody (Ab) could have antimetastatic effects.
Methods
The differential gene expression was measured in a parental cell line, HPC-3, and an established highly liver metastatic cell line, HPC-3H4. This study investigated the effect of OPN RNAi and anti-OPN Ab on the metastatic ability of HPC-3H4 to the liver. An OPN RNAi-expressing vector was introduced into HPC-3H4 cells (HPC-3H4/miOPN), in which OPN production was reduced to the level of the parental HPC-3 cells. Finally, the ability of anti-OPN Ab to suppress liver metastasis was investigated.
Results
Osteopontin was upregulated 11.1-fold in HPC-3H4 in comparison to HPC-3. The metastatic rate of HPC-3H4/miOPN was significantly reduced to 25% in comparison to the 100% metastatic rate of HPC-3H4 and control HPC-3H4/miNeg cells (
P
< 0.01). The metastatic rate of the group given anti-OPN Ab was 50%.
Conclusion
OPN RNAi and anti-OPN Ab had remarkable inhibitory effects against liver metastasis by the pancreatic cancer cell line. |
| doi_str_mv | 10.1007/s00595-009-4082-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733443593</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733443593</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-2d42352aa3390ed01998491ead314ee0f1dded8a198de40a9aa8c64720a41683</originalsourceid><addsrcrecordid>eNp9kE1rHDEMhk1oSDZJf0AvxbeeJpU_dnd8LKEfgUAvuRvF1mS9zNhT2xM21_7yetm0x4KRJfToRXoZ-yDgVgBsPxeAtVl3AKbT0MvucMZWQqtNJ3uh3rEVGC06IY24ZFel7AGk7gEu2KUEpYzpzYr9vo-78BRqSJGngadSKc0p1hB5Jr84KnwML5T5RBVLe6Ecud0yYeQzRpcJa3DctbRRB4rpOeNQCw9xT66Sb0lNvO6Il3kkiq3myKe0FGrR03jDzgccC71_-6_Z47evj3c_uoef3-_vvjx0TplN7aTXUq0lYlsdyINoB2gjCL0SmggG4T35HoXpPWlAg9i7jd5KQC02vbpmn06yc06_FirVTqE4GkeM1JaxW6W0VmujGilOpMuplEyDnXOYML9aAfZovD0Zb5vx9mi8PbSZj2_qy9NE_t_EX6cbIE9Aaa34TNnu05JjO_g_qn8AZ92RcA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733443593</pqid></control><display><type>article</type><title>Inhibition of osteopontin reduces liver metastasis of human pancreatic cancer xenografts injected into the spleen in a mouse model</title><source>MEDLINE</source><source>SpringerLink (Online service)</source><creator>Ohno, Keisuke ; Nishimori, Hidefumi ; Yasoshima, Takahiro ; Kamiguchi, Kenjiro ; Hata, Fumitake ; Fukui, Rika ; Okuya, Koichi ; Kimura, Yasutoshi ; Denno, Ryuichi ; Kon, Shigeyuki ; Uede, Toshimitsu ; Sato, Noriyuki ; Hirata, Koichi</creator><creatorcontrib>Ohno, Keisuke ; Nishimori, Hidefumi ; Yasoshima, Takahiro ; Kamiguchi, Kenjiro ; Hata, Fumitake ; Fukui, Rika ; Okuya, Koichi ; Kimura, Yasutoshi ; Denno, Ryuichi ; Kon, Shigeyuki ; Uede, Toshimitsu ; Sato, Noriyuki ; Hirata, Koichi</creatorcontrib><description>Purpose
Pancreatic cancer is associated with the poorest prognosis of any digestive cancer due to the high incidence of liver metastasis. This study evaluated the possibility that osteopontin (OPN) RNA interference (RNAi) and anti-OPN antibody (Ab) could have antimetastatic effects.
Methods
The differential gene expression was measured in a parental cell line, HPC-3, and an established highly liver metastatic cell line, HPC-3H4. This study investigated the effect of OPN RNAi and anti-OPN Ab on the metastatic ability of HPC-3H4 to the liver. An OPN RNAi-expressing vector was introduced into HPC-3H4 cells (HPC-3H4/miOPN), in which OPN production was reduced to the level of the parental HPC-3 cells. Finally, the ability of anti-OPN Ab to suppress liver metastasis was investigated.
Results
Osteopontin was upregulated 11.1-fold in HPC-3H4 in comparison to HPC-3. The metastatic rate of HPC-3H4/miOPN was significantly reduced to 25% in comparison to the 100% metastatic rate of HPC-3H4 and control HPC-3H4/miNeg cells (
P
< 0.01). The metastatic rate of the group given anti-OPN Ab was 50%.
Conclusion
OPN RNAi and anti-OPN Ab had remarkable inhibitory effects against liver metastasis by the pancreatic cancer cell line.</description><identifier>ISSN: 0941-1291</identifier><identifier>EISSN: 1436-2813</identifier><identifier>DOI: 10.1007/s00595-009-4082-x</identifier><identifier>PMID: 20339989</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Animals ; Antibodies - pharmacology ; Cell Line, Tumor ; Enzyme-Linked Immunosorbent Assay ; Female ; Gene Expression ; Genetic Vectors ; Humans ; Liver Neoplasms - prevention & control ; Liver Neoplasms - secondary ; Medicine ; Medicine & Public Health ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Microarray Analysis ; Original Article ; Osteopontin - genetics ; Osteopontin - immunology ; Osteopontin - physiology ; Pancreatic Neoplasms - pathology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; RNA Interference ; Surgery ; Surgical Oncology ; Transfection ; Transplantation, Heterologous</subject><ispartof>Surgery today (Tokyo, Japan), 2010-04, Vol.40 (4), p.347-356</ispartof><rights>Springer 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-2d42352aa3390ed01998491ead314ee0f1dded8a198de40a9aa8c64720a41683</citedby><cites>FETCH-LOGICAL-c396t-2d42352aa3390ed01998491ead314ee0f1dded8a198de40a9aa8c64720a41683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00595-009-4082-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00595-009-4082-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20339989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohno, Keisuke</creatorcontrib><creatorcontrib>Nishimori, Hidefumi</creatorcontrib><creatorcontrib>Yasoshima, Takahiro</creatorcontrib><creatorcontrib>Kamiguchi, Kenjiro</creatorcontrib><creatorcontrib>Hata, Fumitake</creatorcontrib><creatorcontrib>Fukui, Rika</creatorcontrib><creatorcontrib>Okuya, Koichi</creatorcontrib><creatorcontrib>Kimura, Yasutoshi</creatorcontrib><creatorcontrib>Denno, Ryuichi</creatorcontrib><creatorcontrib>Kon, Shigeyuki</creatorcontrib><creatorcontrib>Uede, Toshimitsu</creatorcontrib><creatorcontrib>Sato, Noriyuki</creatorcontrib><creatorcontrib>Hirata, Koichi</creatorcontrib><title>Inhibition of osteopontin reduces liver metastasis of human pancreatic cancer xenografts injected into the spleen in a mouse model</title><title>Surgery today (Tokyo, Japan)</title><addtitle>Surg Today</addtitle><addtitle>Surg Today</addtitle><description>Purpose
Pancreatic cancer is associated with the poorest prognosis of any digestive cancer due to the high incidence of liver metastasis. This study evaluated the possibility that osteopontin (OPN) RNA interference (RNAi) and anti-OPN antibody (Ab) could have antimetastatic effects.
Methods
The differential gene expression was measured in a parental cell line, HPC-3, and an established highly liver metastatic cell line, HPC-3H4. This study investigated the effect of OPN RNAi and anti-OPN Ab on the metastatic ability of HPC-3H4 to the liver. An OPN RNAi-expressing vector was introduced into HPC-3H4 cells (HPC-3H4/miOPN), in which OPN production was reduced to the level of the parental HPC-3 cells. Finally, the ability of anti-OPN Ab to suppress liver metastasis was investigated.
Results
Osteopontin was upregulated 11.1-fold in HPC-3H4 in comparison to HPC-3. The metastatic rate of HPC-3H4/miOPN was significantly reduced to 25% in comparison to the 100% metastatic rate of HPC-3H4 and control HPC-3H4/miNeg cells (
P
< 0.01). The metastatic rate of the group given anti-OPN Ab was 50%.
Conclusion
OPN RNAi and anti-OPN Ab had remarkable inhibitory effects against liver metastasis by the pancreatic cancer cell line.</description><subject>Animals</subject><subject>Antibodies - pharmacology</subject><subject>Cell Line, Tumor</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Humans</subject><subject>Liver Neoplasms - prevention & control</subject><subject>Liver Neoplasms - secondary</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Microarray Analysis</subject><subject>Original Article</subject><subject>Osteopontin - genetics</subject><subject>Osteopontin - immunology</subject><subject>Osteopontin - physiology</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Transfection</subject><subject>Transplantation, Heterologous</subject><issn>0941-1291</issn><issn>1436-2813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rHDEMhk1oSDZJf0AvxbeeJpU_dnd8LKEfgUAvuRvF1mS9zNhT2xM21_7yetm0x4KRJfToRXoZ-yDgVgBsPxeAtVl3AKbT0MvucMZWQqtNJ3uh3rEVGC06IY24ZFel7AGk7gEu2KUEpYzpzYr9vo-78BRqSJGngadSKc0p1hB5Jr84KnwML5T5RBVLe6Ecud0yYeQzRpcJa3DctbRRB4rpOeNQCw9xT66Sb0lNvO6Il3kkiq3myKe0FGrR03jDzgccC71_-6_Z47evj3c_uoef3-_vvjx0TplN7aTXUq0lYlsdyINoB2gjCL0SmggG4T35HoXpPWlAg9i7jd5KQC02vbpmn06yc06_FirVTqE4GkeM1JaxW6W0VmujGilOpMuplEyDnXOYML9aAfZovD0Zb5vx9mi8PbSZj2_qy9NE_t_EX6cbIE9Aaa34TNnu05JjO_g_qn8AZ92RcA</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Ohno, Keisuke</creator><creator>Nishimori, Hidefumi</creator><creator>Yasoshima, Takahiro</creator><creator>Kamiguchi, Kenjiro</creator><creator>Hata, Fumitake</creator><creator>Fukui, Rika</creator><creator>Okuya, Koichi</creator><creator>Kimura, Yasutoshi</creator><creator>Denno, Ryuichi</creator><creator>Kon, Shigeyuki</creator><creator>Uede, Toshimitsu</creator><creator>Sato, Noriyuki</creator><creator>Hirata, Koichi</creator><general>Springer Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100401</creationdate><title>Inhibition of osteopontin reduces liver metastasis of human pancreatic cancer xenografts injected into the spleen in a mouse model</title><author>Ohno, Keisuke ; Nishimori, Hidefumi ; Yasoshima, Takahiro ; Kamiguchi, Kenjiro ; Hata, Fumitake ; Fukui, Rika ; Okuya, Koichi ; Kimura, Yasutoshi ; Denno, Ryuichi ; Kon, Shigeyuki ; Uede, Toshimitsu ; Sato, Noriyuki ; Hirata, Koichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-2d42352aa3390ed01998491ead314ee0f1dded8a198de40a9aa8c64720a41683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibodies - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Genetic Vectors</topic><topic>Humans</topic><topic>Liver Neoplasms - prevention & control</topic><topic>Liver Neoplasms - secondary</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Microarray Analysis</topic><topic>Original Article</topic><topic>Osteopontin - genetics</topic><topic>Osteopontin - immunology</topic><topic>Osteopontin - physiology</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Transfection</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohno, Keisuke</creatorcontrib><creatorcontrib>Nishimori, Hidefumi</creatorcontrib><creatorcontrib>Yasoshima, Takahiro</creatorcontrib><creatorcontrib>Kamiguchi, Kenjiro</creatorcontrib><creatorcontrib>Hata, Fumitake</creatorcontrib><creatorcontrib>Fukui, Rika</creatorcontrib><creatorcontrib>Okuya, Koichi</creatorcontrib><creatorcontrib>Kimura, Yasutoshi</creatorcontrib><creatorcontrib>Denno, Ryuichi</creatorcontrib><creatorcontrib>Kon, Shigeyuki</creatorcontrib><creatorcontrib>Uede, Toshimitsu</creatorcontrib><creatorcontrib>Sato, Noriyuki</creatorcontrib><creatorcontrib>Hirata, Koichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Surgery today (Tokyo, Japan)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohno, Keisuke</au><au>Nishimori, Hidefumi</au><au>Yasoshima, Takahiro</au><au>Kamiguchi, Kenjiro</au><au>Hata, Fumitake</au><au>Fukui, Rika</au><au>Okuya, Koichi</au><au>Kimura, Yasutoshi</au><au>Denno, Ryuichi</au><au>Kon, Shigeyuki</au><au>Uede, Toshimitsu</au><au>Sato, Noriyuki</au><au>Hirata, Koichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of osteopontin reduces liver metastasis of human pancreatic cancer xenografts injected into the spleen in a mouse model</atitle><jtitle>Surgery today (Tokyo, Japan)</jtitle><stitle>Surg Today</stitle><addtitle>Surg Today</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>40</volume><issue>4</issue><spage>347</spage><epage>356</epage><pages>347-356</pages><issn>0941-1291</issn><eissn>1436-2813</eissn><abstract>Purpose
Pancreatic cancer is associated with the poorest prognosis of any digestive cancer due to the high incidence of liver metastasis. This study evaluated the possibility that osteopontin (OPN) RNA interference (RNAi) and anti-OPN antibody (Ab) could have antimetastatic effects.
Methods
The differential gene expression was measured in a parental cell line, HPC-3, and an established highly liver metastatic cell line, HPC-3H4. This study investigated the effect of OPN RNAi and anti-OPN Ab on the metastatic ability of HPC-3H4 to the liver. An OPN RNAi-expressing vector was introduced into HPC-3H4 cells (HPC-3H4/miOPN), in which OPN production was reduced to the level of the parental HPC-3 cells. Finally, the ability of anti-OPN Ab to suppress liver metastasis was investigated.
Results
Osteopontin was upregulated 11.1-fold in HPC-3H4 in comparison to HPC-3. The metastatic rate of HPC-3H4/miOPN was significantly reduced to 25% in comparison to the 100% metastatic rate of HPC-3H4 and control HPC-3H4/miNeg cells (
P
< 0.01). The metastatic rate of the group given anti-OPN Ab was 50%.
Conclusion
OPN RNAi and anti-OPN Ab had remarkable inhibitory effects against liver metastasis by the pancreatic cancer cell line.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>20339989</pmid><doi>10.1007/s00595-009-4082-x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Surgery today (Tokyo, Japan), 2010-04, Vol.40 (4), p.347-356 |
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| subjects | Animals Antibodies - pharmacology Cell Line, Tumor Enzyme-Linked Immunosorbent Assay Female Gene Expression Genetic Vectors Humans Liver Neoplasms - prevention & control Liver Neoplasms - secondary Medicine Medicine & Public Health Mice Mice, Inbred BALB C Mice, Nude Microarray Analysis Original Article Osteopontin - genetics Osteopontin - immunology Osteopontin - physiology Pancreatic Neoplasms - pathology Reverse Transcriptase Polymerase Chain Reaction RNA RNA Interference Surgery Surgical Oncology Transfection Transplantation, Heterologous |
| title | Inhibition of osteopontin reduces liver metastasis of human pancreatic cancer xenografts injected into the spleen in a mouse model |
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