Id proteins are overexpressed in human oral squamous cell carcinomas
Background: The helix‐loop‐helix (HLH) proteins Id‐1, Id‐2 and Id‐3 have been demonstrated to inhibit the activity of transcription factors and play an important role in regulating cell growth and tissue‐specific differentiation. Methods: To elucidate the involvement of Id in human oral squamous c...
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| Veröffentlicht in: | Journal of oral pathology & medicine 2003-07, Vol.32 (6), p.350-357 |
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| container_title | Journal of oral pathology & medicine |
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| creator | Nishimine, Masayoshi Nakamura, Mitsutoshi Mishima, Kenji Kishi, Munehiro Kirita, Tadaaki Sugimura, Masahito Konishi, Noboru |
| description | Background: The helix‐loop‐helix (HLH) proteins Id‐1, Id‐2 and Id‐3 have been demonstrated to inhibit the activity of transcription factors and play an important role in regulating cell growth and tissue‐specific differentiation.
Methods: To elucidate the involvement of Id in human oral squamous cell carcinoma (OSCC), we examined 83 surgical specimens and eight normal gingival mucosae for the expression of Id proteins by immunohistochemistry; in addition, some specimens of the OSCC and the normal gingivae were also examined for the expression of Id‐1 mRNA by in situ hybridization (ISH), while Western blots were performed on six of the tumours and on cell lysates of five OSCC cell lines. We also explored the correlation between Id expressions and cellular proliferation indicating Ki‐67 or clinical parameters.
Results: We discovered a higher and more frequent expression of Id‐1 protein (27.7%) in human OSCC compared to that of Id‐2 and Id‐3 proteins (6.0 and 7.2%, respectively). Western blot analysis detected Id‐1 protein in four of six tumour samples and in all cell lines. ISH demonstrated strong cytoplasmic localization of Id‐1 mRNA in tumour samples at significantly higher levels compared to those of normal gingival mucosae.
Conclusion: Id‐1 protein expression significantly correlates with lymph node status, indicating that increased Id expression may relate to the metastatic behaviour in human OSCC. |
| doi_str_mv | 10.1034/j.1600-0714.2003.00078.x |
| format | Article |
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Methods: To elucidate the involvement of Id in human oral squamous cell carcinoma (OSCC), we examined 83 surgical specimens and eight normal gingival mucosae for the expression of Id proteins by immunohistochemistry; in addition, some specimens of the OSCC and the normal gingivae were also examined for the expression of Id‐1 mRNA by in situ hybridization (ISH), while Western blots were performed on six of the tumours and on cell lysates of five OSCC cell lines. We also explored the correlation between Id expressions and cellular proliferation indicating Ki‐67 or clinical parameters.
Results: We discovered a higher and more frequent expression of Id‐1 protein (27.7%) in human OSCC compared to that of Id‐2 and Id‐3 proteins (6.0 and 7.2%, respectively). Western blot analysis detected Id‐1 protein in four of six tumour samples and in all cell lines. ISH demonstrated strong cytoplasmic localization of Id‐1 mRNA in tumour samples at significantly higher levels compared to those of normal gingival mucosae.
Conclusion: Id‐1 protein expression significantly correlates with lymph node status, indicating that increased Id expression may relate to the metastatic behaviour in human OSCC.</description><identifier>ISSN: 0904-2512</identifier><identifier>EISSN: 1600-0714</identifier><identifier>DOI: 10.1034/j.1600-0714.2003.00078.x</identifier><identifier>PMID: 12787042</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Blotting, Western ; Carcinoma, Squamous Cell - chemistry ; Carcinoma, Squamous Cell - metabolism ; Dentistry ; Female ; Gene Expression Regulation, Neoplastic ; Helix-Loop-Helix Motifs ; Humans ; Id-1 ; Id-2 ; Id-3 ; Immunohistochemistry ; In Situ Hybridization ; Inhibitor of Differentiation Protein 1 ; Lymphatic Metastasis ; Male ; Medical sciences ; Middle Aged ; Mouth Mucosa - chemistry ; Mouth Mucosa - metabolism ; Mouth Neoplasms - chemistry ; Mouth Neoplasms - metabolism ; Neoplasm Proteins - analysis ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - chemistry ; oral squamous cell carcinoma ; Otorhinolaryngology. Stomatology ; Repressor Proteins ; RNA, Messenger - analysis ; Transcription Factors - analysis ; Transcription Factors - biosynthesis ; Transcription Factors - chemistry ; Tumor Cells, Cultured ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Journal of oral pathology & medicine, 2003-07, Vol.32 (6), p.350-357</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4988-8719f2ad05d25fb1513776ba3f361e153328d73245fb2e20ef06ae6fc4cad7673</citedby><cites>FETCH-LOGICAL-c4988-8719f2ad05d25fb1513776ba3f361e153328d73245fb2e20ef06ae6fc4cad7673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1034%2Fj.1600-0714.2003.00078.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1600-0714.2003.00078.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14862220$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12787042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishimine, Masayoshi</creatorcontrib><creatorcontrib>Nakamura, Mitsutoshi</creatorcontrib><creatorcontrib>Mishima, Kenji</creatorcontrib><creatorcontrib>Kishi, Munehiro</creatorcontrib><creatorcontrib>Kirita, Tadaaki</creatorcontrib><creatorcontrib>Sugimura, Masahito</creatorcontrib><creatorcontrib>Konishi, Noboru</creatorcontrib><title>Id proteins are overexpressed in human oral squamous cell carcinomas</title><title>Journal of oral pathology & medicine</title><addtitle>J Oral Pathol Med</addtitle><description>Background: The helix‐loop‐helix (HLH) proteins Id‐1, Id‐2 and Id‐3 have been demonstrated to inhibit the activity of transcription factors and play an important role in regulating cell growth and tissue‐specific differentiation.
Methods: To elucidate the involvement of Id in human oral squamous cell carcinoma (OSCC), we examined 83 surgical specimens and eight normal gingival mucosae for the expression of Id proteins by immunohistochemistry; in addition, some specimens of the OSCC and the normal gingivae were also examined for the expression of Id‐1 mRNA by in situ hybridization (ISH), while Western blots were performed on six of the tumours and on cell lysates of five OSCC cell lines. We also explored the correlation between Id expressions and cellular proliferation indicating Ki‐67 or clinical parameters.
Results: We discovered a higher and more frequent expression of Id‐1 protein (27.7%) in human OSCC compared to that of Id‐2 and Id‐3 proteins (6.0 and 7.2%, respectively). Western blot analysis detected Id‐1 protein in four of six tumour samples and in all cell lines. ISH demonstrated strong cytoplasmic localization of Id‐1 mRNA in tumour samples at significantly higher levels compared to those of normal gingival mucosae.
Conclusion: Id‐1 protein expression significantly correlates with lymph node status, indicating that increased Id expression may relate to the metastatic behaviour in human OSCC.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Carcinoma, Squamous Cell - chemistry</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Dentistry</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Helix-Loop-Helix Motifs</subject><subject>Humans</subject><subject>Id-1</subject><subject>Id-2</subject><subject>Id-3</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Inhibitor of Differentiation Protein 1</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mouth Mucosa - chemistry</subject><subject>Mouth Mucosa - metabolism</subject><subject>Mouth Neoplasms - chemistry</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Neoplasm Proteins - analysis</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - chemistry</subject><subject>oral squamous cell carcinoma</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Repressor Proteins</subject><subject>RNA, Messenger - analysis</subject><subject>Transcription Factors - analysis</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - chemistry</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>0904-2512</issn><issn>1600-0714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1P3DAQhq0KVBboX0C-lFvS8UfirNRLBS0fWi0gqNqbNetMRLb5WOxNu_z7OuwKrpw80jzv-NXDGBeQClD6yzIVOUACRuhUAqgUAEyRbj6wyetij01gCjqRmZAH7DCEJYAwSouP7EBIUxjQcsLOr0q-8v2a6i5w9MT7v-Rps_IUApW87vjj0GLHe48ND08Dtv0QuKOm4Q69q7u-xXDM9itsAn3avUfs54_vD2eXyezm4urs2yxxeloUSWHEtJJYQlbKrFqITChj8gWqSuWCRKaULEqjpI5LSRKoghwpr5x2WJrcqCN2ur0bGz8NFNa2rcPYBTuKtaxRSqvcjGCxBZ3vQ_BU2ZWvW_TPVoAdDdqlHUXZUZQdDdoXg3YToye7P4ZFS-VbcKcsAp93AAaHTeWxc3V443SRSykhcl-33L-6oed3F7DXN7dxiPFkG6_DmjavcfR_bDRhMvtrfmHvpvfwG-6Vnav_NViaOw</recordid><startdate>200307</startdate><enddate>200307</enddate><creator>Nishimine, Masayoshi</creator><creator>Nakamura, Mitsutoshi</creator><creator>Mishima, Kenji</creator><creator>Kishi, Munehiro</creator><creator>Kirita, Tadaaki</creator><creator>Sugimura, Masahito</creator><creator>Konishi, Noboru</creator><general>Munksgaard International Publishers</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200307</creationdate><title>Id proteins are overexpressed in human oral squamous cell carcinomas</title><author>Nishimine, Masayoshi ; Nakamura, Mitsutoshi ; Mishima, Kenji ; Kishi, Munehiro ; Kirita, Tadaaki ; Sugimura, Masahito ; Konishi, Noboru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4988-8719f2ad05d25fb1513776ba3f361e153328d73245fb2e20ef06ae6fc4cad7673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Carcinoma, Squamous Cell - chemistry</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Dentistry</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Helix-Loop-Helix Motifs</topic><topic>Humans</topic><topic>Id-1</topic><topic>Id-2</topic><topic>Id-3</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Inhibitor of Differentiation Protein 1</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mouth Mucosa - chemistry</topic><topic>Mouth Mucosa - metabolism</topic><topic>Mouth Neoplasms - chemistry</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Neoplasm Proteins - analysis</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - chemistry</topic><topic>oral squamous cell carcinoma</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Repressor Proteins</topic><topic>RNA, Messenger - analysis</topic><topic>Transcription Factors - analysis</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - chemistry</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishimine, Masayoshi</creatorcontrib><creatorcontrib>Nakamura, Mitsutoshi</creatorcontrib><creatorcontrib>Mishima, Kenji</creatorcontrib><creatorcontrib>Kishi, Munehiro</creatorcontrib><creatorcontrib>Kirita, Tadaaki</creatorcontrib><creatorcontrib>Sugimura, Masahito</creatorcontrib><creatorcontrib>Konishi, Noboru</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of oral pathology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishimine, Masayoshi</au><au>Nakamura, Mitsutoshi</au><au>Mishima, Kenji</au><au>Kishi, Munehiro</au><au>Kirita, Tadaaki</au><au>Sugimura, Masahito</au><au>Konishi, Noboru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Id proteins are overexpressed in human oral squamous cell carcinomas</atitle><jtitle>Journal of oral pathology & medicine</jtitle><addtitle>J Oral Pathol Med</addtitle><date>2003-07</date><risdate>2003</risdate><volume>32</volume><issue>6</issue><spage>350</spage><epage>357</epage><pages>350-357</pages><issn>0904-2512</issn><eissn>1600-0714</eissn><abstract>Background: The helix‐loop‐helix (HLH) proteins Id‐1, Id‐2 and Id‐3 have been demonstrated to inhibit the activity of transcription factors and play an important role in regulating cell growth and tissue‐specific differentiation.
Methods: To elucidate the involvement of Id in human oral squamous cell carcinoma (OSCC), we examined 83 surgical specimens and eight normal gingival mucosae for the expression of Id proteins by immunohistochemistry; in addition, some specimens of the OSCC and the normal gingivae were also examined for the expression of Id‐1 mRNA by in situ hybridization (ISH), while Western blots were performed on six of the tumours and on cell lysates of five OSCC cell lines. We also explored the correlation between Id expressions and cellular proliferation indicating Ki‐67 or clinical parameters.
Results: We discovered a higher and more frequent expression of Id‐1 protein (27.7%) in human OSCC compared to that of Id‐2 and Id‐3 proteins (6.0 and 7.2%, respectively). Western blot analysis detected Id‐1 protein in four of six tumour samples and in all cell lines. ISH demonstrated strong cytoplasmic localization of Id‐1 mRNA in tumour samples at significantly higher levels compared to those of normal gingival mucosae.
Conclusion: Id‐1 protein expression significantly correlates with lymph node status, indicating that increased Id expression may relate to the metastatic behaviour in human OSCC.</abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>12787042</pmid><doi>10.1034/j.1600-0714.2003.00078.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Biological and medical sciences Blotting, Western Carcinoma, Squamous Cell - chemistry Carcinoma, Squamous Cell - metabolism Dentistry Female Gene Expression Regulation, Neoplastic Helix-Loop-Helix Motifs Humans Id-1 Id-2 Id-3 Immunohistochemistry In Situ Hybridization Inhibitor of Differentiation Protein 1 Lymphatic Metastasis Male Medical sciences Middle Aged Mouth Mucosa - chemistry Mouth Mucosa - metabolism Mouth Neoplasms - chemistry Mouth Neoplasms - metabolism Neoplasm Proteins - analysis Neoplasm Proteins - biosynthesis Neoplasm Proteins - chemistry oral squamous cell carcinoma Otorhinolaryngology. Stomatology Repressor Proteins RNA, Messenger - analysis Transcription Factors - analysis Transcription Factors - biosynthesis Transcription Factors - chemistry Tumor Cells, Cultured Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Id proteins are overexpressed in human oral squamous cell carcinomas |
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