Intracellular [Ca 2+] and K + and Cl − efflux responses in submandibular cells of neonatal and adult rats
The effects of graded doses (5 × 10 −8 to 10 −5 ) acetylcholine on intracellular Ca 2+ and on 86Rb and 36Cl efflux were compared in submandibular cell clusters of 1 and 7 day-old and adult rats. Initial Ca 2+ peaks were similar at agonists concentrations lower than 10 −7 M but the release of Rb + an...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-11, Vol.188 (3), p.1161-1168 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Seagrave, J.C. Ramsey, R.aChaney Barker, S. Martinez, J.R. |
| description | The effects of graded doses (5 × 10
−8 to 10
−5 ) acetylcholine on intracellular Ca
2+ and on
86Rb and
36Cl efflux were compared in submandibular cell clusters of 1 and 7 day-old and adult rats. Initial Ca
2+ peaks were similar at agonists concentrations lower than 10
−7 M but the release of Rb
+ and Cl
− were smaller in cells of young animals.At higher agonist concentrations, Ca
2+ peaks were higher in immature cells; however, initial Cl
− (but not Rb
+) efflux was similar to that of mature cells. Plateau Ca
2+ levels were independent of age and agonist concentrations but the content of Cl
− and Rb
+ varied greatly and differences between age groups were less evident. These data confirm a dissociation between intracellular Ca
2+ levels and Ca
2+-mediated ion transport in immature salivary cells. |
| doi_str_mv | 10.1016/0006-291X(92)91353-R |
| format | Article |
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−8 to 10
−5 ) acetylcholine on intracellular Ca
2+ and on
86Rb and
36Cl efflux were compared in submandibular cell clusters of 1 and 7 day-old and adult rats. Initial Ca
2+ peaks were similar at agonists concentrations lower than 10
−7 M but the release of Rb
+ and Cl
− were smaller in cells of young animals.At higher agonist concentrations, Ca
2+ peaks were higher in immature cells; however, initial Cl
− (but not Rb
+) efflux was similar to that of mature cells. Plateau Ca
2+ levels were independent of age and agonist concentrations but the content of Cl
− and Rb
+ varied greatly and differences between age groups were less evident. These data confirm a dissociation between intracellular Ca
2+ levels and Ca
2+-mediated ion transport in immature salivary cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(92)91353-R</identifier><identifier>PMID: 1445350</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Acetylcholine - pharmacology ; Aging ; Animals ; Animals, Newborn ; Biological and medical sciences ; Calcium - metabolism ; Chlorides - metabolism ; Fluorometry ; Fundamental and applied biological sciences. Psychology ; Fura-2 ; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus ; Radioisotopes - metabolism ; Rats ; Rats, Sprague-Dawley ; Rubidium Radioisotopes - metabolism ; Submandibular Gland - cytology ; Submandibular Gland - drug effects ; Submandibular Gland - metabolism ; Time Factors ; Vertebrates: digestive system</subject><ispartof>Biochemical and biophysical research communications, 1992-11, Vol.188 (3), p.1161-1168</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-e00663290d285435fef0aa457d07eb78a136e2554aa1e80b9f580e3da5175bc93</citedby><cites>FETCH-LOGICAL-c386t-e00663290d285435fef0aa457d07eb78a136e2554aa1e80b9f580e3da5175bc93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(92)91353-R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4443363$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1445350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seagrave, J.C.</creatorcontrib><creatorcontrib>Ramsey, R.aChaney</creatorcontrib><creatorcontrib>Barker, S.</creatorcontrib><creatorcontrib>Martinez, J.R.</creatorcontrib><title>Intracellular [Ca 2+] and K + and Cl − efflux responses in submandibular cells of neonatal and adult rats</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The effects of graded doses (5 × 10
−8 to 10
−5 ) acetylcholine on intracellular Ca
2+ and on
86Rb and
36Cl efflux were compared in submandibular cell clusters of 1 and 7 day-old and adult rats. Initial Ca
2+ peaks were similar at agonists concentrations lower than 10
−7 M but the release of Rb
+ and Cl
− were smaller in cells of young animals.At higher agonist concentrations, Ca
2+ peaks were higher in immature cells; however, initial Cl
− (but not Rb
+) efflux was similar to that of mature cells. Plateau Ca
2+ levels were independent of age and agonist concentrations but the content of Cl
− and Rb
+ varied greatly and differences between age groups were less evident. These data confirm a dissociation between intracellular Ca
2+ levels and Ca
2+-mediated ion transport in immature salivary cells.</description><subject>Acetylcholine - pharmacology</subject><subject>Aging</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Chlorides - metabolism</subject><subject>Fluorometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fura-2</subject><subject>Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus</subject><subject>Radioisotopes - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rubidium Radioisotopes - metabolism</subject><subject>Submandibular Gland - cytology</subject><subject>Submandibular Gland - drug effects</subject><subject>Submandibular Gland - metabolism</subject><subject>Time Factors</subject><subject>Vertebrates: digestive system</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9qFEEQhxtR4ibxDRT6IJIQJvbfmemLIEuiIYFAiCCINDU91dDaO7N2z0h8g5zziD6JM7tLcvNUh_p-xa8-Ql5zdsoZL98zxspCGP71yIhjw6WWxc0zsuDMsEJwpp6TxSPykuzn_IMxzlVp9sgeV0pLzRbk50U3JHAY4xgh0W9LoOLkO4WupZf0ZDOXkf69f6DofRzvaMK87ruMmYaO5rFZTUhoNuH5Sqa9px32HQwQN3FoxzjQBEM-JC88xIyvdvOAfDk_u11-Lq6uP10sP14VTtblUOBUupTCsFbUWknt0TMApauWVdhUNXBZotBaAXCsWWO8rhnKFjSvdOOMPCDvtnfXqf81Yh7sKuS5HEzFxmwrKZUUagbVFnSpzzmht-sUVpD-WM7s7NjOAu0s0BphN47tzRR7s7s_vY_tU2grddq_3e0hO4g-QedCfsSUUlKWcsI-bDGcXPwOmGx2ATuHbUjoBtv24f89_gGrHpe8</recordid><startdate>19921116</startdate><enddate>19921116</enddate><creator>Seagrave, J.C.</creator><creator>Ramsey, R.aChaney</creator><creator>Barker, S.</creator><creator>Martinez, J.R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921116</creationdate><title>Intracellular [Ca 2+] and K + and Cl − efflux responses in submandibular cells of neonatal and adult rats</title><author>Seagrave, J.C. ; Ramsey, R.aChaney ; Barker, S. ; Martinez, J.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-e00663290d285435fef0aa457d07eb78a136e2554aa1e80b9f580e3da5175bc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acetylcholine - pharmacology</topic><topic>Aging</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Chlorides - metabolism</topic><topic>Fluorometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fura-2</topic><topic>Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus</topic><topic>Radioisotopes - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rubidium Radioisotopes - metabolism</topic><topic>Submandibular Gland - cytology</topic><topic>Submandibular Gland - drug effects</topic><topic>Submandibular Gland - metabolism</topic><topic>Time Factors</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seagrave, J.C.</creatorcontrib><creatorcontrib>Ramsey, R.aChaney</creatorcontrib><creatorcontrib>Barker, S.</creatorcontrib><creatorcontrib>Martinez, J.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seagrave, J.C.</au><au>Ramsey, R.aChaney</au><au>Barker, S.</au><au>Martinez, J.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracellular [Ca 2+] and K + and Cl − efflux responses in submandibular cells of neonatal and adult rats</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-11-16</date><risdate>1992</risdate><volume>188</volume><issue>3</issue><spage>1161</spage><epage>1168</epage><pages>1161-1168</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The effects of graded doses (5 × 10
−8 to 10
−5 ) acetylcholine on intracellular Ca
2+ and on
86Rb and
36Cl efflux were compared in submandibular cell clusters of 1 and 7 day-old and adult rats. Initial Ca
2+ peaks were similar at agonists concentrations lower than 10
−7 M but the release of Rb
+ and Cl
− were smaller in cells of young animals.At higher agonist concentrations, Ca
2+ peaks were higher in immature cells; however, initial Cl
− (but not Rb
+) efflux was similar to that of mature cells. Plateau Ca
2+ levels were independent of age and agonist concentrations but the content of Cl
− and Rb
+ varied greatly and differences between age groups were less evident. These data confirm a dissociation between intracellular Ca
2+ levels and Ca
2+-mediated ion transport in immature salivary cells.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1445350</pmid><doi>10.1016/0006-291X(92)91353-R</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1992-11, Vol.188 (3), p.1161-1168 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73343249 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetylcholine - pharmacology Aging Animals Animals, Newborn Biological and medical sciences Calcium - metabolism Chlorides - metabolism Fluorometry Fundamental and applied biological sciences. Psychology Fura-2 Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus Radioisotopes - metabolism Rats Rats, Sprague-Dawley Rubidium Radioisotopes - metabolism Submandibular Gland - cytology Submandibular Gland - drug effects Submandibular Gland - metabolism Time Factors Vertebrates: digestive system |
| title | Intracellular [Ca 2+] and K + and Cl − efflux responses in submandibular cells of neonatal and adult rats |
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