Expression of recombinant endochitinase from the Antarctic bacterium, Sanguibacter antarcticus KOPRI 21702 in Pichia pastoris by codon optimization

An endochitinase was previously purified and the gene was cloned from the psychrophilic Antarctic bacterium, Sanguibacter antarcticus (KCTC 13143). In the present study, recombinant endochitinase, rChi21702, was expressed using a yeast expression system (Pichia pastoris) and codon optimization. The...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Protein expression and purification 2010-05, Vol.71 (1), p.108-114
Hauptverfasser:	Lee, Sung Gu, Koh, Hye Yeon, Han, Se Jong, Park, Heeyong, Na, Deuk Chae, Kim, Il-Chan, Lee, Hong Kum, Yim, Joung Han
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antarctic Regions Antarctica Bacteria - drug effects Bacteria - enzymology Base Sequence Bioreactors - microbiology Chitinases - chemistry Chitinases - genetics Chitinases - isolation & purification Chitinases - metabolism Chromatography, Thin Layer Codon - genetics Codon optimization Endochitinases Genetic Techniques Hydrogen-Ion Concentration - drug effects Methanol - pharmacology Molecular Sequence Data Pichia - metabolism Pichia pastoris Recombinant Recombinant Proteins - metabolism Recombination, Genetic - drug effects Temperature
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!