Pistil drip following pollination: a simple in planta Agrobacterium-mediated transformation in cotton

Transgenic cotton plants were developed by pistil drip inoculation in a solution containing Agrobacterium carrying a gene for resistance to the herbicide Basta (bar), 10% (w/v) sucrose, 0.05% (v/v) Silwet L-77 and 40 mg acetosyringone l⁻¹. Pistil drip during 17:00-19:00 on the first day of flowering...

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Veröffentlicht in:	Biotechnology letters 2010-04, Vol.32 (4), p.547-555
Hauptverfasser:	TianZi, Chen, ShenJie, Wu, Jun, Zhao, WangZhen, Guo, TianZhen, Zhang
Format:	Artikel
Sprache:	eng
Schlagworte:	Agricultural biotechnology Aminobutyrates - pharmacology Bacterial Proteins Biological and medical sciences Biotechnology Blotting, Southern Cotton Drug Resistance, Bacterial Flowers - physiology Fundamental and applied biological sciences. Psychology Genes Gossypium - drug effects Gossypium - genetics Gossypium - metabolism Herbicides Plant reproduction Plant resistance Pollination Polymerase Chain Reaction Rhizobium - genetics Time Factors Transformation, Genetic Transgenes Transgenic plants
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