Enhanced Resistance to Helicoverpa zea in Tobacco Expressing an Activated Form of Maize Ribosome- Inactivating Protein
Progeny of two transgenic tobacco (Nicotiana tabacum L.) lines that expressed an activated form of maize (Zea mays L.) ribosome-inactivating protein (RIP) had varying resistance to the insect species tested. A subset of R2 plants from the two lines appeared to be more resistant to larvae of the ciga...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2003-06, Vol.51 (12), p.3568-3574 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Dowd, Patrick F Zuo, Wei-Neng Gillikin, Jeffery W Johnson, Eric T Boston, Rebecca S |
| description | Progeny of two transgenic tobacco (Nicotiana tabacum L.) lines that expressed an activated form of maize (Zea mays L.) ribosome-inactivating protein (RIP) had varying resistance to the insect species tested. A subset of R2 plants from the two lines appeared to be more resistant to larvae of the cigarette beetle, Lasioderma serricorne (F.), and the tobacco hornworm, Manduca sexta (L.) than the wild type plants. Progeny (R3) of the more resistant R2 plants were tested more extensively for insect resistance. Resistance to the corn earworm, Helicoverpa zea (Boddie), was most consistent, with significantly decreased feeding often accompanied by increased mortality and reduced weights of survivors fed on leaf disks of the two transgenic lines compared to the wild type. The amount of damage by H. zea was significantly inversely correlated with levels of RIP. Resistance of RIP-producing plants to H. zea was greater than expected on the basis of prior in vitro results using diet-incorporated maize RIP. The R3 transgenic plant leaf disks were also often more resistant to feeding by larvae of L. serricorne compared to wild type plants. Although reduced feeding by M. sexta was noted when they were fed leaf disks from transgenic compared to wild type plants the first day of exposure, differences were not significant. This information provides further support for maize RIP having a role in resistance to maize-feeding insects. Keywords: Corn earworm; tobacco hornworm; Manduca; cigarette beetle; Lasioderma |
| doi_str_mv | 10.1021/jf0211433 |
| format | Article |
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A subset of R2 plants from the two lines appeared to be more resistant to larvae of the cigarette beetle, Lasioderma serricorne (F.), and the tobacco hornworm, Manduca sexta (L.) than the wild type plants. Progeny (R3) of the more resistant R2 plants were tested more extensively for insect resistance. Resistance to the corn earworm, Helicoverpa zea (Boddie), was most consistent, with significantly decreased feeding often accompanied by increased mortality and reduced weights of survivors fed on leaf disks of the two transgenic lines compared to the wild type. The amount of damage by H. zea was significantly inversely correlated with levels of RIP. Resistance of RIP-producing plants to H. zea was greater than expected on the basis of prior in vitro results using diet-incorporated maize RIP. The R3 transgenic plant leaf disks were also often more resistant to feeding by larvae of L. serricorne compared to wild type plants. Although reduced feeding by M. sexta was noted when they were fed leaf disks from transgenic compared to wild type plants the first day of exposure, differences were not significant. This information provides further support for maize RIP having a role in resistance to maize-feeding insects. Keywords: Corn earworm; tobacco hornworm; Manduca; cigarette beetle; Lasioderma</description><identifier>ISSN: 0021-8561</identifier><identifier>ISSN: 1520-5118</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf0211433</identifier><identifier>PMID: 12769526</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Agronomy. Soil science and plant productions ; Animals ; Biological and medical sciences ; Biotechnology ; Coleoptera ; corn ; Feeding Behavior ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Genetics and breeding of economic plants ; Helicoverpa zea ; insect pests ; Larva ; Lasioderma serricorne ; Manduca ; Manduca sexta ; Methods. Procedures. Technologies ; mortality ; Moths ; N-glycoside hydrolases ; Nicotiana - genetics ; Nicotiana - metabolism ; Nicotiana tabacum ; Pest animals ; Pest resistance ; phytophagous insects ; Plant Extracts - genetics ; Plant Extracts - metabolism ; plant pests ; plant proteins ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Ribosome Inactivating Proteins ; ribosome-inactivating protein ; tobacco ; transgenes ; Transgenic animals and transgenic plants ; Transgenic plants ; Varietal selection. Specialized plant breeding, plant breeding aims ; Zea mays</subject><ispartof>Journal of agricultural and food chemistry, 2003-06, Vol.51 (12), p.3568-3574</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a434t-7bbe3bc7c5a4131ecf46010875837b1fe06fe21a8c02c2c2d63980d97c563ad63</citedby><cites>FETCH-LOGICAL-a434t-7bbe3bc7c5a4131ecf46010875837b1fe06fe21a8c02c2c2d63980d97c563ad63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf0211433$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf0211433$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,778,782,2754,27059,27907,27908,56721,56771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14849892$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12769526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dowd, Patrick F</creatorcontrib><creatorcontrib>Zuo, Wei-Neng</creatorcontrib><creatorcontrib>Gillikin, Jeffery W</creatorcontrib><creatorcontrib>Johnson, Eric T</creatorcontrib><creatorcontrib>Boston, Rebecca S</creatorcontrib><title>Enhanced Resistance to Helicoverpa zea in Tobacco Expressing an Activated Form of Maize Ribosome- Inactivating Protein</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Progeny of two transgenic tobacco (Nicotiana tabacum L.) lines that expressed an activated form of maize (Zea mays L.) ribosome-inactivating protein (RIP) had varying resistance to the insect species tested. A subset of R2 plants from the two lines appeared to be more resistant to larvae of the cigarette beetle, Lasioderma serricorne (F.), and the tobacco hornworm, Manduca sexta (L.) than the wild type plants. Progeny (R3) of the more resistant R2 plants were tested more extensively for insect resistance. Resistance to the corn earworm, Helicoverpa zea (Boddie), was most consistent, with significantly decreased feeding often accompanied by increased mortality and reduced weights of survivors fed on leaf disks of the two transgenic lines compared to the wild type. The amount of damage by H. zea was significantly inversely correlated with levels of RIP. Resistance of RIP-producing plants to H. zea was greater than expected on the basis of prior in vitro results using diet-incorporated maize RIP. The R3 transgenic plant leaf disks were also often more resistant to feeding by larvae of L. serricorne compared to wild type plants. Although reduced feeding by M. sexta was noted when they were fed leaf disks from transgenic compared to wild type plants the first day of exposure, differences were not significant. This information provides further support for maize RIP having a role in resistance to maize-feeding insects. Keywords: Corn earworm; tobacco hornworm; Manduca; cigarette beetle; Lasioderma</description><subject>Agronomy. Soil science and plant productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Coleoptera</subject><subject>corn</subject><subject>Feeding Behavior</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetics and breeding of economic plants</subject><subject>Helicoverpa zea</subject><subject>insect pests</subject><subject>Larva</subject><subject>Lasioderma serricorne</subject><subject>Manduca</subject><subject>Manduca sexta</subject><subject>Methods. Procedures. Technologies</subject><subject>mortality</subject><subject>Moths</subject><subject>N-glycoside hydrolases</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana tabacum</subject><subject>Pest animals</subject><subject>Pest resistance</subject><subject>phytophagous insects</subject><subject>Plant Extracts - genetics</subject><subject>Plant Extracts - metabolism</subject><subject>plant pests</subject><subject>plant proteins</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Ribosome Inactivating Proteins</subject><subject>ribosome-inactivating protein</subject><subject>tobacco</subject><subject>transgenes</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>Zea mays</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQhiMEYsvCgT8AvoDEITATJ7FzXFbdD6mIVduFozVxncUljbt2Wi3763GUantBQpbGHvuZ1_a8SfIW4TNChl_WTYyYc_4smWCRQVogyufJBOJ2KosST5JXIawBQBYCXiYnmImyKrJykuyn3S_qtFmxuQk29MOa9Y5dmdZqtzd-S-zRELMdW7qatHZs-rD1JgTb3THq2Jnu7Z76KHDh_Ia5hn0j-2jY3NYuuI1J2XVHIzNU3HjXG9u9Tl401Abz5jCfJrcX0-X5VTr7fnl9fjZLKed5n4q6NrzWQheUI0ejm7wEBCkKyUWNjYGyMRmS1JDpOFYlrySsqlhQcorZafJx1N16d78zoVcbG7RpW-qM2wUlOM8BMfsviEIIABwUP42g9i4Ebxq19XZD_o9CUIMb6smNyL47iO7qjVkdyUP7I_DhAFDQ1DY-tt-GI5fLvJLV8Lp05KJD5uHpnPxvVQouCrW8WahZ_vWSL-Y_1M_Ivx_5hpyiOx81bxcZIAeUFYgMjzeTDmrtdr6LNvzjC38BPeS1Ww</recordid><startdate>20030604</startdate><enddate>20030604</enddate><creator>Dowd, Patrick F</creator><creator>Zuo, Wei-Neng</creator><creator>Gillikin, Jeffery W</creator><creator>Johnson, Eric T</creator><creator>Boston, Rebecca S</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030604</creationdate><title>Enhanced Resistance to Helicoverpa zea in Tobacco Expressing an Activated Form of Maize Ribosome- Inactivating Protein</title><author>Dowd, Patrick F ; Zuo, Wei-Neng ; Gillikin, Jeffery W ; Johnson, Eric T ; Boston, Rebecca S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a434t-7bbe3bc7c5a4131ecf46010875837b1fe06fe21a8c02c2c2d63980d97c563ad63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Coleoptera</topic><topic>corn</topic><topic>Feeding Behavior</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetics and breeding of economic plants</topic><topic>Helicoverpa zea</topic><topic>insect pests</topic><topic>Larva</topic><topic>Lasioderma serricorne</topic><topic>Manduca</topic><topic>Manduca sexta</topic><topic>Methods. Procedures. Technologies</topic><topic>mortality</topic><topic>Moths</topic><topic>N-glycoside hydrolases</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana tabacum</topic><topic>Pest animals</topic><topic>Pest resistance</topic><topic>phytophagous insects</topic><topic>Plant Extracts - genetics</topic><topic>Plant Extracts - metabolism</topic><topic>plant pests</topic><topic>plant proteins</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Ribosome Inactivating Proteins</topic><topic>ribosome-inactivating protein</topic><topic>tobacco</topic><topic>transgenes</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dowd, Patrick F</creatorcontrib><creatorcontrib>Zuo, Wei-Neng</creatorcontrib><creatorcontrib>Gillikin, Jeffery W</creatorcontrib><creatorcontrib>Johnson, Eric T</creatorcontrib><creatorcontrib>Boston, Rebecca S</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dowd, Patrick F</au><au>Zuo, Wei-Neng</au><au>Gillikin, Jeffery W</au><au>Johnson, Eric T</au><au>Boston, Rebecca S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Resistance to Helicoverpa zea in Tobacco Expressing an Activated Form of Maize Ribosome- Inactivating Protein</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2003-06-04</date><risdate>2003</risdate><volume>51</volume><issue>12</issue><spage>3568</spage><epage>3574</epage><pages>3568-3574</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Progeny of two transgenic tobacco (Nicotiana tabacum L.) lines that expressed an activated form of maize (Zea mays L.) ribosome-inactivating protein (RIP) had varying resistance to the insect species tested. A subset of R2 plants from the two lines appeared to be more resistant to larvae of the cigarette beetle, Lasioderma serricorne (F.), and the tobacco hornworm, Manduca sexta (L.) than the wild type plants. Progeny (R3) of the more resistant R2 plants were tested more extensively for insect resistance. Resistance to the corn earworm, Helicoverpa zea (Boddie), was most consistent, with significantly decreased feeding often accompanied by increased mortality and reduced weights of survivors fed on leaf disks of the two transgenic lines compared to the wild type. The amount of damage by H. zea was significantly inversely correlated with levels of RIP. Resistance of RIP-producing plants to H. zea was greater than expected on the basis of prior in vitro results using diet-incorporated maize RIP. The R3 transgenic plant leaf disks were also often more resistant to feeding by larvae of L. serricorne compared to wild type plants. Although reduced feeding by M. sexta was noted when they were fed leaf disks from transgenic compared to wild type plants the first day of exposure, differences were not significant. This information provides further support for maize RIP having a role in resistance to maize-feeding insects. Keywords: Corn earworm; tobacco hornworm; Manduca; cigarette beetle; Lasioderma</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>12769526</pmid><doi>10.1021/jf0211433</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2003-06, Vol.51 (12), p.3568-3574 |
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| subjects | Agronomy. Soil science and plant productions Animals Biological and medical sciences Biotechnology Coleoptera corn Feeding Behavior Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetics and breeding of economic plants Helicoverpa zea insect pests Larva Lasioderma serricorne Manduca Manduca sexta Methods. Procedures. Technologies mortality Moths N-glycoside hydrolases Nicotiana - genetics Nicotiana - metabolism Nicotiana tabacum Pest animals Pest resistance phytophagous insects Plant Extracts - genetics Plant Extracts - metabolism plant pests plant proteins Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Ribosome Inactivating Proteins ribosome-inactivating protein tobacco transgenes Transgenic animals and transgenic plants Transgenic plants Varietal selection. Specialized plant breeding, plant breeding aims Zea mays |
| title | Enhanced Resistance to Helicoverpa zea in Tobacco Expressing an Activated Form of Maize Ribosome- Inactivating Protein |
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