Novel restricted access chiral stationary phase synthesized via atom transfer radical polymerization for the analysis of chiral drugs in biological matrices
A new restricted access chiral stationary phase was designed and synthesized via atom transfer radical polymerization (ATRP). Surface modified bi-functional silica with chiral selector in the inner layer and hydrophilic external layer was prepared by taking advantage of the controlled/living propert...
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| Veröffentlicht in: | Analyst (London) 2010-01, Vol.135 (7), p.1785-1792 |
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| creator | Wang, Huaisong Xu, Dan Jiang, Ping Zhang, Min Dong, Xiangchao |
| description | A new restricted access chiral stationary phase was designed and synthesized
via
atom transfer radical polymerization (ATRP). Surface modified bi-functional silica with chiral selector in the inner layer and hydrophilic external layer was prepared by taking advantage of the controlled/living property of the ATRP method. ATRP initiator bound porous silica was synthesized to perform surface initiated polymerization. The chiral stationary phase was then synthesized by grafting poly(glycidyl methacrylate) (pGMA) on the surface of the silica through "grafting from" polymerization followed by β-cyclodextrin (β-CD) immobilization. On the surface of this β-CD immobilized material, the external pGMA layer was synthesized
via
second round ATRP using the initiator on the material. The hydrophilic structure was formed after the hydrolysis, which created a diffusion barrier for proteins. The new chiral restricted access material (chiral-CD-RAM) was characterized and its abilities for chiral separation and protein exclusion were evaluated. The result demonstrates that
enantio
-separations can be achieved for several drugs in HPLC using chiral-CD-RAM as stationary phase. Meanwhile, good protein recovery has been obtained. It indicated that this chiral-CD-RAM can be used for determination of certain chiral drugs in biological samples with direct injection in the HPLC analysis.
This multifunctional material will facilitate the separation and determination of chiral compounds in biological samples with direct injection in HPLC analysis |
| doi_str_mv | 10.1039/c0an00050g |
| format | Article |
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via
atom transfer radical polymerization (ATRP). Surface modified bi-functional silica with chiral selector in the inner layer and hydrophilic external layer was prepared by taking advantage of the controlled/living property of the ATRP method. ATRP initiator bound porous silica was synthesized to perform surface initiated polymerization. The chiral stationary phase was then synthesized by grafting poly(glycidyl methacrylate) (pGMA) on the surface of the silica through "grafting from" polymerization followed by β-cyclodextrin (β-CD) immobilization. On the surface of this β-CD immobilized material, the external pGMA layer was synthesized
via
second round ATRP using the initiator on the material. The hydrophilic structure was formed after the hydrolysis, which created a diffusion barrier for proteins. The new chiral restricted access material (chiral-CD-RAM) was characterized and its abilities for chiral separation and protein exclusion were evaluated. The result demonstrates that
enantio
-separations can be achieved for several drugs in HPLC using chiral-CD-RAM as stationary phase. Meanwhile, good protein recovery has been obtained. It indicated that this chiral-CD-RAM can be used for determination of certain chiral drugs in biological samples with direct injection in the HPLC analysis.
This multifunctional material will facilitate the separation and determination of chiral compounds in biological samples with direct injection in HPLC analysis</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c0an00050g</identifier><identifier>PMID: 20498907</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Analytical chemistry ; beta-Cyclodextrins - chemistry ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, High Pressure Liquid - methods ; Epoxy Compounds - chemistry ; Exact sciences and technology ; Free Radicals - chemistry ; Methacrylates - chemistry ; Other chromatographic methods ; Pharmaceutical Preparations - chemistry ; Pharmaceutical Preparations - isolation & purification ; Polymers - chemistry ; Porosity ; Silicon Dioxide - chemistry ; Stereoisomerism</subject><ispartof>Analyst (London), 2010-01, Vol.135 (7), p.1785-1792</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-8d7038a7ddb56f51834867654317d0e69bcbaafc8fd4ed3596d3b413546c84363</citedby><cites>FETCH-LOGICAL-c462t-8d7038a7ddb56f51834867654317d0e69bcbaafc8fd4ed3596d3b413546c84363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,2832,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23014897$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20498907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Huaisong</creatorcontrib><creatorcontrib>Xu, Dan</creatorcontrib><creatorcontrib>Jiang, Ping</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Dong, Xiangchao</creatorcontrib><title>Novel restricted access chiral stationary phase synthesized via atom transfer radical polymerization for the analysis of chiral drugs in biological matrices</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>A new restricted access chiral stationary phase was designed and synthesized
via
atom transfer radical polymerization (ATRP). Surface modified bi-functional silica with chiral selector in the inner layer and hydrophilic external layer was prepared by taking advantage of the controlled/living property of the ATRP method. ATRP initiator bound porous silica was synthesized to perform surface initiated polymerization. The chiral stationary phase was then synthesized by grafting poly(glycidyl methacrylate) (pGMA) on the surface of the silica through "grafting from" polymerization followed by β-cyclodextrin (β-CD) immobilization. On the surface of this β-CD immobilized material, the external pGMA layer was synthesized
via
second round ATRP using the initiator on the material. The hydrophilic structure was formed after the hydrolysis, which created a diffusion barrier for proteins. The new chiral restricted access material (chiral-CD-RAM) was characterized and its abilities for chiral separation and protein exclusion were evaluated. The result demonstrates that
enantio
-separations can be achieved for several drugs in HPLC using chiral-CD-RAM as stationary phase. Meanwhile, good protein recovery has been obtained. It indicated that this chiral-CD-RAM can be used for determination of certain chiral drugs in biological samples with direct injection in the HPLC analysis.
This multifunctional material will facilitate the separation and determination of chiral compounds in biological samples with direct injection in HPLC analysis</description><subject>Analytical chemistry</subject><subject>beta-Cyclodextrins - chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Epoxy Compounds - chemistry</subject><subject>Exact sciences and technology</subject><subject>Free Radicals - chemistry</subject><subject>Methacrylates - chemistry</subject><subject>Other chromatographic methods</subject><subject>Pharmaceutical Preparations - chemistry</subject><subject>Pharmaceutical Preparations - isolation & purification</subject><subject>Polymers - chemistry</subject><subject>Porosity</subject><subject>Silicon Dioxide - chemistry</subject><subject>Stereoisomerism</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1ERbeFC3eQOSCkSgF7_RHnWFUUKlVwgXM08ceuURIHT7bS9rfwY_F2t616gZNlz_POjN-XkNecfeRMNJ8sg5ExptjqGVlwoWWl1NI8J4vyKKqlVvKYnCD-KldeqBfkeMlkYxpWL8ifb-nG9zR7nHO0s3cUrPWI1K5jhp7iDHNMI-QtndaAnuJ2nNce421BbyJQmNNA5wwjBp9pBhdtkU2p3w4-x9s7NQ0p06KiMEK_xYg0hfsBLm9WSONIu5j6tLpTD7BbxuNLchSgR__qcJ6Sn5eff1x8ra6_f7m6OL-urNTLuTKuZsJA7VyndFDcCGl0Xb4teO2Y101nO4BgTXDSO6Ea7UQnuVBSWyOFFqfkw77vlNPvTbGiHSJa3_cw-rTBtlZC17UsDf9LCiGaRktVyLM9aXNCzD60U45D8bHlrN3F1j7GVuC3h7abbvDuAb3PqQDvDwBgcSgUv23ER04wLk2z497suYz2ofpk0Lt_1dvJBfEXuI64mg</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Wang, Huaisong</creator><creator>Xu, Dan</creator><creator>Jiang, Ping</creator><creator>Zhang, Min</creator><creator>Dong, Xiangchao</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100101</creationdate><title>Novel restricted access chiral stationary phase synthesized via atom transfer radical polymerization for the analysis of chiral drugs in biological matrices</title><author>Wang, Huaisong ; Xu, Dan ; Jiang, Ping ; Zhang, Min ; Dong, Xiangchao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-8d7038a7ddb56f51834867654317d0e69bcbaafc8fd4ed3596d3b413546c84363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical chemistry</topic><topic>beta-Cyclodextrins - chemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Epoxy Compounds - chemistry</topic><topic>Exact sciences and technology</topic><topic>Free Radicals - chemistry</topic><topic>Methacrylates - chemistry</topic><topic>Other chromatographic methods</topic><topic>Pharmaceutical Preparations - chemistry</topic><topic>Pharmaceutical Preparations - isolation & purification</topic><topic>Polymers - chemistry</topic><topic>Porosity</topic><topic>Silicon Dioxide - chemistry</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Huaisong</creatorcontrib><creatorcontrib>Xu, Dan</creatorcontrib><creatorcontrib>Jiang, Ping</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Dong, Xiangchao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Huaisong</au><au>Xu, Dan</au><au>Jiang, Ping</au><au>Zhang, Min</au><au>Dong, Xiangchao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel restricted access chiral stationary phase synthesized via atom transfer radical polymerization for the analysis of chiral drugs in biological matrices</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>135</volume><issue>7</issue><spage>1785</spage><epage>1792</epage><pages>1785-1792</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><coden>ANALAO</coden><abstract>A new restricted access chiral stationary phase was designed and synthesized
via
atom transfer radical polymerization (ATRP). Surface modified bi-functional silica with chiral selector in the inner layer and hydrophilic external layer was prepared by taking advantage of the controlled/living property of the ATRP method. ATRP initiator bound porous silica was synthesized to perform surface initiated polymerization. The chiral stationary phase was then synthesized by grafting poly(glycidyl methacrylate) (pGMA) on the surface of the silica through "grafting from" polymerization followed by β-cyclodextrin (β-CD) immobilization. On the surface of this β-CD immobilized material, the external pGMA layer was synthesized
via
second round ATRP using the initiator on the material. The hydrophilic structure was formed after the hydrolysis, which created a diffusion barrier for proteins. The new chiral restricted access material (chiral-CD-RAM) was characterized and its abilities for chiral separation and protein exclusion were evaluated. The result demonstrates that
enantio
-separations can be achieved for several drugs in HPLC using chiral-CD-RAM as stationary phase. Meanwhile, good protein recovery has been obtained. It indicated that this chiral-CD-RAM can be used for determination of certain chiral drugs in biological samples with direct injection in the HPLC analysis.
This multifunctional material will facilitate the separation and determination of chiral compounds in biological samples with direct injection in HPLC analysis</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>20498907</pmid><doi>10.1039/c0an00050g</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analytical chemistry beta-Cyclodextrins - chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Epoxy Compounds - chemistry Exact sciences and technology Free Radicals - chemistry Methacrylates - chemistry Other chromatographic methods Pharmaceutical Preparations - chemistry Pharmaceutical Preparations - isolation & purification Polymers - chemistry Porosity Silicon Dioxide - chemistry Stereoisomerism |
| title | Novel restricted access chiral stationary phase synthesized via atom transfer radical polymerization for the analysis of chiral drugs in biological matrices |
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