Transfected endometrial cultured cells: A system to study gene regulation by estrogens
Glandular epithelial (GE) and stromal cells were isolated from guinea-pig endometrium, cultured and subcultured separately. At the end of subculture, the purity of each cell population was higher than 95% and cells displayed a high level of estrogen receptors. Calcium phosphate transfection conditio...
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| Veröffentlicht in: | Molecular and cellular endocrinology 1992-09, Vol.87 (1), p.R11-R17 |
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| container_title | Molecular and cellular endocrinology |
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| creator | Vuillermoz, C. Jouvenot, M. Pellerin, I. Ordener, C. Royez, M. Adessi, G.L. |
| description | Glandular epithelial (GE) and stromal cells were isolated from guinea-pig endometrium, cultured and subcultured separately. At the end of subculture, the purity of each cell population was higher than 95% and cells displayed a high level of estrogen receptors. Calcium phosphate transfection conditions were defined using a control plasmid containing the bacterial CAT gene driven by viral promoter and enhancer sequences. Transfection experiments were performed with other plasmids in which CAT gene was linked to different estrogen response elements (EREs) derived from those of vitellogenin genes. CAT activity was significantly increased by estradiol-17β treatment only when GE or stromal cells were transfected with plasmids containing EREs previously reported as functional EREs in other cell types. This induction was abolished by ICI 164,384 diethylstilbestrol was as effective as estradiol-17β for CAT induction and estradiol-17α was ineffective. Transiently transfected endometrial cells in subculture are a suitable system to study the estrogen effect on gene regulatory elements. |
| doi_str_mv | 10.1016/0303-7207(92)90250-A |
| format | Article |
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At the end of subculture, the purity of each cell population was higher than 95% and cells displayed a high level of estrogen receptors. Calcium phosphate transfection conditions were defined using a control plasmid containing the bacterial CAT gene driven by viral promoter and enhancer sequences. Transfection experiments were performed with other plasmids in which CAT gene was linked to different estrogen response elements (EREs) derived from those of vitellogenin genes. CAT activity was significantly increased by estradiol-17β treatment only when GE or stromal cells were transfected with plasmids containing EREs previously reported as functional EREs in other cell types. This induction was abolished by ICI 164,384 diethylstilbestrol was as effective as estradiol-17β for CAT induction and estradiol-17α was ineffective. Transiently transfected endometrial cells in subculture are a suitable system to study the estrogen effect on gene regulatory elements.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/0303-7207(92)90250-A</identifier><identifier>PMID: 1446780</identifier><identifier>CODEN: MCEND6</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cell culture ; Cells, Cultured ; Connective Tissue Cells ; Endometrium ; Endometrium - cytology ; Endometrium - metabolism ; Enzyme Induction - drug effects ; Epithelial Cells ; Estradiol - analogs & derivatives ; Estradiol - pharmacology ; Estrogen ; Female ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation - drug effects ; Gene regulation ; Guinea Pigs ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Polyunsaturated Alkamides ; Proto-Oncogene Proteins c-fos - genetics ; Proto-Oncogene Proteins c-fos - physiology ; Recombinant Fusion Proteins - biosynthesis ; Regulatory Sequences, Nucleic Acid ; Transfection ; Uterus</subject><ispartof>Molecular and cellular endocrinology, 1992-09, Vol.87 (1), p.R11-R17</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-3a760ec5fa0e82b4bb39a0ab07c8c55c1b73483fa1fd3a2ac3b335fc5ff4e6ff3</citedby><cites>FETCH-LOGICAL-c386t-3a760ec5fa0e82b4bb39a0ab07c8c55c1b73483fa1fd3a2ac3b335fc5ff4e6ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0303-7207(92)90250-A$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5516031$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1446780$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vuillermoz, C.</creatorcontrib><creatorcontrib>Jouvenot, M.</creatorcontrib><creatorcontrib>Pellerin, I.</creatorcontrib><creatorcontrib>Ordener, C.</creatorcontrib><creatorcontrib>Royez, M.</creatorcontrib><creatorcontrib>Adessi, G.L.</creatorcontrib><title>Transfected endometrial cultured cells: A system to study gene regulation by estrogens</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Glandular epithelial (GE) and stromal cells were isolated from guinea-pig endometrium, cultured and subcultured separately. At the end of subculture, the purity of each cell population was higher than 95% and cells displayed a high level of estrogen receptors. Calcium phosphate transfection conditions were defined using a control plasmid containing the bacterial CAT gene driven by viral promoter and enhancer sequences. Transfection experiments were performed with other plasmids in which CAT gene was linked to different estrogen response elements (EREs) derived from those of vitellogenin genes. CAT activity was significantly increased by estradiol-17β treatment only when GE or stromal cells were transfected with plasmids containing EREs previously reported as functional EREs in other cell types. This induction was abolished by ICI 164,384 diethylstilbestrol was as effective as estradiol-17β for CAT induction and estradiol-17α was ineffective. Transiently transfected endometrial cells in subculture are a suitable system to study the estrogen effect on gene regulatory elements.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell culture</subject><subject>Cells, Cultured</subject><subject>Connective Tissue Cells</subject><subject>Endometrium</subject><subject>Endometrium - cytology</subject><subject>Endometrium - metabolism</subject><subject>Enzyme Induction - drug effects</subject><subject>Epithelial Cells</subject><subject>Estradiol - analogs & derivatives</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene regulation</subject><subject>Guinea Pigs</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Polyunsaturated Alkamides</subject><subject>Proto-Oncogene Proteins c-fos - genetics</subject><subject>Proto-Oncogene Proteins c-fos - physiology</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Transfection</subject><subject>Uterus</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo67j6DxRyENFDa6Wr0-n2IAyLX7DgZfUa0unKEumPNZUW5t-bcYb15qmg6nmrikeI5wreKlDtO0DAytRgXvf1mx5qDdX-gdipztRVB9o8FLt75LF4wvwTAIyuuwtxoZqmNR3sxI-b5BYO5DONkpZxnSmn6CbptylvqTQ9TRO_l3vJB840y7xKztt4kLe0kEx0u00ux3WRw0ES57SWPj8Vj4KbmJ6d66X4_unjzdWX6vrb569X--vKY9fmCp1pgbwODqirh2YYsHfgBjC-81p7NRhsOgxOhRFd7TwOiDqUQGioDQEvxavT3ru0_trKeTtHPn7sFlo3tgYRe9NgAZsT6NPKnCjYuxRnlw5WgT3qtEdX9ujK9rX9q9PuS-zFef82zDT-C538lfnL89yxd1MoMn3ke0xr1QKqgn04YVRc_I6ULPtIi6cxpqLejmv8_x9_AA2ZkoY</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Vuillermoz, C.</creator><creator>Jouvenot, M.</creator><creator>Pellerin, I.</creator><creator>Ordener, C.</creator><creator>Royez, M.</creator><creator>Adessi, G.L.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920901</creationdate><title>Transfected endometrial cultured cells: A system to study gene regulation by estrogens</title><author>Vuillermoz, C. ; Jouvenot, M. ; Pellerin, I. ; Ordener, C. ; Royez, M. ; Adessi, G.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-3a760ec5fa0e82b4bb39a0ab07c8c55c1b73483fa1fd3a2ac3b335fc5ff4e6ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell culture</topic><topic>Cells, Cultured</topic><topic>Connective Tissue Cells</topic><topic>Endometrium</topic><topic>Endometrium - cytology</topic><topic>Endometrium - metabolism</topic><topic>Enzyme Induction - drug effects</topic><topic>Epithelial Cells</topic><topic>Estradiol - analogs & derivatives</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene regulation</topic><topic>Guinea Pigs</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Polyunsaturated Alkamides</topic><topic>Proto-Oncogene Proteins c-fos - genetics</topic><topic>Proto-Oncogene Proteins c-fos - physiology</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Transfection</topic><topic>Uterus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vuillermoz, C.</creatorcontrib><creatorcontrib>Jouvenot, M.</creatorcontrib><creatorcontrib>Pellerin, I.</creatorcontrib><creatorcontrib>Ordener, C.</creatorcontrib><creatorcontrib>Royez, M.</creatorcontrib><creatorcontrib>Adessi, G.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vuillermoz, C.</au><au>Jouvenot, M.</au><au>Pellerin, I.</au><au>Ordener, C.</au><au>Royez, M.</au><au>Adessi, G.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transfected endometrial cultured cells: A system to study gene regulation by estrogens</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>87</volume><issue>1</issue><spage>R11</spage><epage>R17</epage><pages>R11-R17</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><coden>MCEND6</coden><abstract>Glandular epithelial (GE) and stromal cells were isolated from guinea-pig endometrium, cultured and subcultured separately. At the end of subculture, the purity of each cell population was higher than 95% and cells displayed a high level of estrogen receptors. Calcium phosphate transfection conditions were defined using a control plasmid containing the bacterial CAT gene driven by viral promoter and enhancer sequences. Transfection experiments were performed with other plasmids in which CAT gene was linked to different estrogen response elements (EREs) derived from those of vitellogenin genes. CAT activity was significantly increased by estradiol-17β treatment only when GE or stromal cells were transfected with plasmids containing EREs previously reported as functional EREs in other cell types. This induction was abolished by ICI 164,384 diethylstilbestrol was as effective as estradiol-17β for CAT induction and estradiol-17α was ineffective. 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| ispartof | Molecular and cellular endocrinology, 1992-09, Vol.87 (1), p.R11-R17 |
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| subjects | Animals Base Sequence Biological and medical sciences Cell culture Cells, Cultured Connective Tissue Cells Endometrium Endometrium - cytology Endometrium - metabolism Enzyme Induction - drug effects Epithelial Cells Estradiol - analogs & derivatives Estradiol - pharmacology Estrogen Female Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation - drug effects Gene regulation Guinea Pigs Molecular and cellular biology Molecular genetics Molecular Sequence Data Polyunsaturated Alkamides Proto-Oncogene Proteins c-fos - genetics Proto-Oncogene Proteins c-fos - physiology Recombinant Fusion Proteins - biosynthesis Regulatory Sequences, Nucleic Acid Transfection Uterus |
| title | Transfected endometrial cultured cells: A system to study gene regulation by estrogens |
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