Rolling Circle Amplification Combined with Gold Nanoparticle Aggregates for Highly Sensitive Identification of Single-Nucleotide Polymorphisms

A highly sensitive and specific colorimetry-based rolling circle amplification (RCA) assay method for single-nucleotide polymorphism genotyping has been developed. A circular template is generated by ligation upon the recognition of a point mutation on DNA targets. An RCA amplification is then initi...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-04, Vol.82 (7), p.2811-2816
Hauptverfasser:	Li, Jishan, Deng, Ting, Chu, Xia, Yang, Ronghua, Jiang, Jianhui, Shen, Guoli, Yu, Ruqin
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemistry Deoxyribonucleic acid DNA DNA Probes - chemistry DNA Probes - genetics Exact sciences and technology Gene amplification Genotype Genotype & phenotype Gold Gold - chemistry Humans Metal Nanoparticles - chemistry Molecules Nanoparticles Nucleic Acid Amplification Techniques - methods Polymorphism Polymorphism, Single Nucleotide Spectrometric and optical methods Spectrophotometry, Ultraviolet Thalassemia - genetics
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