Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots
Sporamin, a sweet potato tuberous storage protein, is a Kunitz-type trypsin inhibitor. Its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. To test its potential as an anti-feedant for the beet cyst nematode (Heterodera schachtii Schm.), the sporam...
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| Veröffentlicht in: | Plant molecular biology 2003-04, Vol.51 (6), p.839-849 |
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| creator | Cai, Daguang Thurau, Tim Tian, Yanyan Lange, Tina Yeh, Kai-Wun Jung, Christian |
| description | Sporamin, a sweet potato tuberous storage protein, is a Kunitz-type trypsin inhibitor. Its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. To test its potential as an anti-feedant for the beet cyst nematode (Heterodera schachtii Schm.), the sporamin gene SpTI-1 was introduced into sugar beet (Beta vulgaris L.) by Agrobacterium rhizogenes-mediated transformation. Twelve different hairy root clones expressing sporamin were selected for studying nematode development. Of these, 8 hairy root clones were found to show significant efficiency in inhibiting the growth and development of the female nematodes whereas 4 root clones did not show any inhibitory effects even though the SpTI-1 gene was regularly expressed in all of the tested hairy roots as revealed by northern and western analyses. Inhibition of nematode development correlated with trypsin inhibitor activity but not with the amount of sporamin expressed in hairy roots. These data demonstrate that the trypsin inhibitor activity is the critical factor for inhibiting growth and development of cyst nematodes in sugar beet hairy roots expressing the sporamin gene. Hence, the sweet potato sporamin can be used as a new and effective anti-feedant for controlling cyst nematodes offering an alternative strategy for establishing nematode resistance in crops. |
| doi_str_mv | 10.1023/a:1023089017906 |
| format | Article |
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Its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. To test its potential as an anti-feedant for the beet cyst nematode (Heterodera schachtii Schm.), the sporamin gene SpTI-1 was introduced into sugar beet (Beta vulgaris L.) by Agrobacterium rhizogenes-mediated transformation. Twelve different hairy root clones expressing sporamin were selected for studying nematode development. Of these, 8 hairy root clones were found to show significant efficiency in inhibiting the growth and development of the female nematodes whereas 4 root clones did not show any inhibitory effects even though the SpTI-1 gene was regularly expressed in all of the tested hairy roots as revealed by northern and western analyses. Inhibition of nematode development correlated with trypsin inhibitor activity but not with the amount of sporamin expressed in hairy roots. These data demonstrate that the trypsin inhibitor activity is the critical factor for inhibiting growth and development of cyst nematodes in sugar beet hairy roots expressing the sporamin gene. Hence, the sweet potato sporamin can be used as a new and effective anti-feedant for controlling cyst nematodes offering an alternative strategy for establishing nematode resistance in crops.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/a:1023089017906</identifier><identifier>PMID: 12777044</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; Beta vulgaris - genetics ; Beta vulgaris - parasitology ; Blotting, Northern ; Blotting, Southern ; Blotting, Western ; Cysts ; DNA, Plant - genetics ; Female ; Gene Dosage ; Gene Expression Regulation, Plant ; Genome, Plant ; Immunity, Innate - genetics ; Male ; Nematoda - growth & development ; Nematodes ; Peptides ; Plant Diseases - genetics ; Plant Diseases - parasitology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Proteins - physiology ; Plant Roots - genetics ; Plant Roots - parasitology ; Plants, Genetically Modified ; Potatoes ; RNA, Plant - genetics ; RNA, Plant - metabolism ; Roots ; Sugar ; Trypsin Inhibitors - genetics ; Trypsin Inhibitors - metabolism ; Trypsin Inhibitors - physiology ; Vegetables</subject><ispartof>Plant molecular biology, 2003-04, Vol.51 (6), p.839-849</ispartof><rights>Kluwer Academic Publishers 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12777044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cai, Daguang</creatorcontrib><creatorcontrib>Thurau, Tim</creatorcontrib><creatorcontrib>Tian, Yanyan</creatorcontrib><creatorcontrib>Lange, Tina</creatorcontrib><creatorcontrib>Yeh, Kai-Wun</creatorcontrib><creatorcontrib>Jung, Christian</creatorcontrib><title>Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Sporamin, a sweet potato tuberous storage protein, is a Kunitz-type trypsin inhibitor. Its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. To test its potential as an anti-feedant for the beet cyst nematode (Heterodera schachtii Schm.), the sporamin gene SpTI-1 was introduced into sugar beet (Beta vulgaris L.) by Agrobacterium rhizogenes-mediated transformation. Twelve different hairy root clones expressing sporamin were selected for studying nematode development. Of these, 8 hairy root clones were found to show significant efficiency in inhibiting the growth and development of the female nematodes whereas 4 root clones did not show any inhibitory effects even though the SpTI-1 gene was regularly expressed in all of the tested hairy roots as revealed by northern and western analyses. Inhibition of nematode development correlated with trypsin inhibitor activity but not with the amount of sporamin expressed in hairy roots. These data demonstrate that the trypsin inhibitor activity is the critical factor for inhibiting growth and development of cyst nematodes in sugar beet hairy roots expressing the sporamin gene. Hence, the sweet potato sporamin can be used as a new and effective anti-feedant for controlling cyst nematodes offering an alternative strategy for establishing nematode resistance in crops.</description><subject>Animals</subject><subject>Beta vulgaris - genetics</subject><subject>Beta vulgaris - parasitology</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Cysts</subject><subject>DNA, Plant - genetics</subject><subject>Female</subject><subject>Gene Dosage</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genome, Plant</subject><subject>Immunity, Innate - genetics</subject><subject>Male</subject><subject>Nematoda - growth & development</subject><subject>Nematodes</subject><subject>Peptides</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - parasitology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Proteins - physiology</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - parasitology</subject><subject>Plants, Genetically Modified</subject><subject>Potatoes</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - metabolism</subject><subject>Roots</subject><subject>Sugar</subject><subject>Trypsin Inhibitors - genetics</subject><subject>Trypsin Inhibitors - metabolism</subject><subject>Trypsin Inhibitors - physiology</subject><subject>Vegetables</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkTFv1TAUhS0Eoo_CzIYsBlSGtHac2E43qIBWelKHwhzdONfETy92sJ1K-Un9lxi1LCxM5-jo07m6OoS85eycs1pcwOUfYbpjXHVMPiM73ipRtazWz8mOcamqpuH1CXmV0oExzpiQL8kJr5VSrGl25OFuCRFm56sZRwcZRxoxuZTBG6Q50AExU7OlTD3OkMOIiZ5dY8ZYbASazARmys7ROzPN5x-pS3TEBf2IPtPgaY7bkpynzk9ucDnEjYLJ7t7lrWQ0rT8hPl45-4wZ6P16LElp2ZeyCVzhYwg5vSYvLBwTvnnSU_Lj65fvV9fV_vbbzdWnfXUQgueqM4ZpiQKVla1sG42Ws8GOcmhFy0cF9dA2shYaBLfM1lYrM9Y4KKstdF0rTsmHx94lhl8rptzPLhk8HsFjWFOvhBC60_8HuVa8k5oV8P0_4CGs0ZcneqVVx4VUqkDvnqB1KFP0S3QzxK3_O5X4DZdOl-s</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Cai, Daguang</creator><creator>Thurau, Tim</creator><creator>Tian, Yanyan</creator><creator>Lange, Tina</creator><creator>Yeh, Kai-Wun</creator><creator>Jung, Christian</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots</title><author>Cai, Daguang ; Thurau, Tim ; Tian, Yanyan ; Lange, Tina ; Yeh, Kai-Wun ; Jung, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j331t-9cc086e3e7f656548ef10bfd6b5351d7a2b546238a31f0f2f87cd2eb7f8fa9953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Beta vulgaris - genetics</topic><topic>Beta vulgaris - parasitology</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Cysts</topic><topic>DNA, Plant - genetics</topic><topic>Female</topic><topic>Gene Dosage</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genome, Plant</topic><topic>Immunity, Innate - genetics</topic><topic>Male</topic><topic>Nematoda - growth & development</topic><topic>Nematodes</topic><topic>Peptides</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - parasitology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Proteins - physiology</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - parasitology</topic><topic>Plants, Genetically Modified</topic><topic>Potatoes</topic><topic>RNA, Plant - genetics</topic><topic>RNA, Plant - metabolism</topic><topic>Roots</topic><topic>Sugar</topic><topic>Trypsin Inhibitors - genetics</topic><topic>Trypsin Inhibitors - metabolism</topic><topic>Trypsin Inhibitors - physiology</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Daguang</creatorcontrib><creatorcontrib>Thurau, Tim</creatorcontrib><creatorcontrib>Tian, Yanyan</creatorcontrib><creatorcontrib>Lange, Tina</creatorcontrib><creatorcontrib>Yeh, Kai-Wun</creatorcontrib><creatorcontrib>Jung, Christian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Daguang</au><au>Thurau, Tim</au><au>Tian, Yanyan</au><au>Lange, Tina</au><au>Yeh, Kai-Wun</au><au>Jung, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>51</volume><issue>6</issue><spage>839</spage><epage>849</epage><pages>839-849</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Sporamin, a sweet potato tuberous storage protein, is a Kunitz-type trypsin inhibitor. Its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. To test its potential as an anti-feedant for the beet cyst nematode (Heterodera schachtii Schm.), the sporamin gene SpTI-1 was introduced into sugar beet (Beta vulgaris L.) by Agrobacterium rhizogenes-mediated transformation. Twelve different hairy root clones expressing sporamin were selected for studying nematode development. Of these, 8 hairy root clones were found to show significant efficiency in inhibiting the growth and development of the female nematodes whereas 4 root clones did not show any inhibitory effects even though the SpTI-1 gene was regularly expressed in all of the tested hairy roots as revealed by northern and western analyses. Inhibition of nematode development correlated with trypsin inhibitor activity but not with the amount of sporamin expressed in hairy roots. These data demonstrate that the trypsin inhibitor activity is the critical factor for inhibiting growth and development of cyst nematodes in sugar beet hairy roots expressing the sporamin gene. Hence, the sweet potato sporamin can be used as a new and effective anti-feedant for controlling cyst nematodes offering an alternative strategy for establishing nematode resistance in crops.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>12777044</pmid><doi>10.1023/a:1023089017906</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Beta vulgaris - genetics Beta vulgaris - parasitology Blotting, Northern Blotting, Southern Blotting, Western Cysts DNA, Plant - genetics Female Gene Dosage Gene Expression Regulation, Plant Genome, Plant Immunity, Innate - genetics Male Nematoda - growth & development Nematodes Peptides Plant Diseases - genetics Plant Diseases - parasitology Plant Proteins - genetics Plant Proteins - metabolism Plant Proteins - physiology Plant Roots - genetics Plant Roots - parasitology Plants, Genetically Modified Potatoes RNA, Plant - genetics RNA, Plant - metabolism Roots Sugar Trypsin Inhibitors - genetics Trypsin Inhibitors - metabolism Trypsin Inhibitors - physiology Vegetables |
| title | Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots |
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