Fluorescence Detection of Telomerase Activity in Cancer Cells Based on Isothermal Circular Strand-Displacement Polymerization Reaction

On the basis of the extension reaction of a telomerase substrate (TS) primer in the presence of the telomerase, the inherent signal-transduction mechanism of the hairpin fluorescence probe, and the strand-displacement property of polymerase, an amplified fluorescence detection of telomerase activity...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-04, Vol.82 (7), p.2850-2855
Hauptverfasser:	Ding, Caifeng, Li, Xiangling, Ge, Ying, Zhang, Shusheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Biological and medical sciences Biomarkers, Tumor - analysis Biomarkers, Tumor - metabolism Cancer Cell Line, Tumor Cell physiology Cells Chemistry DNA, Circular - metabolism Exact sciences and technology Fluorescence Fluorescent Dyes - chemistry Fundamental and applied biological sciences. Psychology HeLa Cells Humans Molecular and cellular biology Molecular genetics Polymerase Chain Reaction Replication Signal transduction Spectrometric and optical methods Spectrometry, Fluorescence - methods Telomerase Telomerase - analysis Telomerase - metabolism
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