Fluorescence displacement method for the determination of receptor-ligand binding constants

The equilibrium constant for the binding of a spectroscopically invisible ligand to its protein receptor can be determined in a competition experiment, by using a structural analog that contains a reporter group (fluorophor). A novel mathematical treatment of the multiple equilibria allows the analy...

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Veröffentlicht in:	Analytical biochemistry 1992-08, Vol.205 (1), p.65-69
Hauptverfasser:	Kuzmič, Petr, Moss, Marcia L., Kofron, James L., Rich, Daniel H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Isomerases - metabolism binding Binding, Competitive Biological and medical sciences Carrier Proteins - metabolism Cyclosporine - metabolism Diverse techniques fluorescence Fluorescent Dyes Fundamental and applied biological sciences. Psychology Kinetics Ligands Molecular and cellular biology Peptidylprolyl Isomerase Proteins - metabolism receptors Receptors, Cell Surface - metabolism Spectrometry, Fluorescence
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