Purification of glucose 6-phosphate dehydrogenase from Buffalo ( Bubalus bubalis) erythrocytes and investigation of some kinetic properties

Glucose 6-phosphate dehydrogenase (G6PD) was purified from buffalo ( Bubalus bubalis) erythrocytes and some characteristics of the enzyme were investigated. The purification procedure was composed of two steps: hemolysate preparation and 2 ′,5 ′-ADP–Sepharose 4B affinity gel chromatography. Thanks t...

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Veröffentlicht in:	Protein expression and purification 2003-06, Vol.29 (2), p.304-310
Hauptverfasser:	Ciftçi, Mehmet, Beydemir, Sükrü, Yilmaz, Hayrullah, Altikat, Sayit
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - pharmacology Adenosine Triphosphate - pharmacology Animals Binding, Competitive Buffalo Buffaloes - blood Chromatography, Affinity Enzyme Inhibitors - metabolism Enzyme Inhibitors - pharmacology Erythrocyte Erythrocytes - enzymology Glucose 6-phosphate dehydrogenase Glucose-6-Phosphate - metabolism Glucosephosphate Dehydrogenase - antagonists & inhibitors Glucosephosphate Dehydrogenase - blood Glucosephosphate Dehydrogenase - chemistry Hydrogen-Ion Concentration Kinetics Molecular Weight NADP - metabolism NADP - pharmacology Purification Temperature
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