Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)
Summary Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transc...
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| Veröffentlicht in: | Molecular microbiology 1992-10, Vol.6 (19), p.2797-2804 |
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| container_title | Molecular microbiology |
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| creator | Takano, E. Gramajo, H. C. Strauch, E. Andres, N. White, J. Bibb, M. J. |
| description | Summary
Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift‐down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth‐phase‐dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters. |
| doi_str_mv | 10.1111/j.1365-2958.1992.tb01459.x |
| format | Article |
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Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift‐down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth‐phase‐dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1992.tb01459.x</identifier><identifier>PMID: 1435258</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Bacterial Proteins ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genes, Regulator ; Genetics ; Guanosine Tetraphosphate - metabolism ; Microbiology ; Molecular Sequence Data ; Prodigiosin - analogs & derivatives ; Prodigiosin - biosynthesis ; Promoter Regions, Genetic ; Streptomyces - genetics ; Streptomyces - growth & development ; Streptomyces - metabolism ; Streptomyces coelicolor ; Trans-Activators - genetics ; Transcription, Genetic</subject><ispartof>Molecular microbiology, 1992-10, Vol.6 (19), p.2797-2804</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5487-ff9185ac943d7ff185c4818233f0ea84c43c8de03e94c69aa318ee4cf661d7673</citedby><cites>FETCH-LOGICAL-c5487-ff9185ac943d7ff185c4818233f0ea84c43c8de03e94c69aa318ee4cf661d7673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1992.tb01459.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1992.tb01459.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4394831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1435258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takano, E.</creatorcontrib><creatorcontrib>Gramajo, H. C.</creatorcontrib><creatorcontrib>Strauch, E.</creatorcontrib><creatorcontrib>Andres, N.</creatorcontrib><creatorcontrib>White, J.</creatorcontrib><creatorcontrib>Bibb, M. J.</creatorcontrib><title>Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift‐down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth‐phase‐dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Genes, Regulator</subject><subject>Genetics</subject><subject>Guanosine Tetraphosphate - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Prodigiosin - analogs & derivatives</subject><subject>Prodigiosin - biosynthesis</subject><subject>Promoter Regions, Genetic</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - growth & development</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces coelicolor</subject><subject>Trans-Activators - genetics</subject><subject>Transcription, Genetic</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd1qFDEUx4Moda0-ghBERC9mTCaZmcQbKfWr0OKFFbwL2czJbpbZyTTJ2O6dj-AT-TA-iRl2qeKNGALn63c-4I_QE0pKmt_LTUlZUxeVrEVJpazKtCSU17K8uYMWt6W7aEFkTQomqi_30YMYN4RQRhp2hI4oZ3VViwX6cRn0EE1wY3J-0D0OsJp6PQfYW5zWkDPdG5z-wrRJ7qtOPuAVDJBD46chRWznTPDXaf3z2_dxrSNk28EIQwdDwmPw3WT-nK6H5JbOJ2fwlBmz62fGrZyPbsD5f0oBxuS3OwMRGw-9M77PW07Y8-rFQ3TP6j7Co4M9Rp_fvb08_VCcf3x_dnpyXpiai7awVlJRayM561prs2-4oKJizBLQghvOjOiAMJDcNFJrRgUAN7ZpaNc2LTtGz_Zz83FXE8Skti4a6Hs9gJ-iahljDW3JP0HasIZJ3mTw1R40wccYwKoxuK0OO0WJmlVWGzVLqWYp1ayyOqisbnLz48OWabmF7nfrXtZcf3qo62h0b7N4xsVbjOcLBKMZe73Hrl0Pu_84QF1cnFWtbNkvj4fMgg</recordid><startdate>199210</startdate><enddate>199210</enddate><creator>Takano, E.</creator><creator>Gramajo, H. C.</creator><creator>Strauch, E.</creator><creator>Andres, N.</creator><creator>White, J.</creator><creator>Bibb, M. J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199210</creationdate><title>Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)</title><author>Takano, E. ; Gramajo, H. C. ; Strauch, E. ; Andres, N. ; White, J. ; Bibb, M. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5487-ff9185ac943d7ff185c4818233f0ea84c43c8de03e94c69aa318ee4cf661d7673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Genes, Regulator</topic><topic>Genetics</topic><topic>Guanosine Tetraphosphate - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Prodigiosin - analogs & derivatives</topic><topic>Prodigiosin - biosynthesis</topic><topic>Promoter Regions, Genetic</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - growth & development</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces coelicolor</topic><topic>Trans-Activators - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takano, E.</creatorcontrib><creatorcontrib>Gramajo, H. C.</creatorcontrib><creatorcontrib>Strauch, E.</creatorcontrib><creatorcontrib>Andres, N.</creatorcontrib><creatorcontrib>White, J.</creatorcontrib><creatorcontrib>Bibb, M. J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takano, E.</au><au>Gramajo, H. C.</au><au>Strauch, E.</au><au>Andres, N.</au><au>White, J.</au><au>Bibb, M. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1992-10</date><risdate>1992</risdate><volume>6</volume><issue>19</issue><spage>2797</spage><epage>2804</epage><pages>2797-2804</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift‐down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth‐phase‐dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1435258</pmid><doi>10.1111/j.1365-2958.1992.tb01459.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0950-382X |
| ispartof | Molecular microbiology, 1992-10, Vol.6 (19), p.2797-2804 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acid Sequence Bacterial Proteins Bacteriology Base Sequence Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Genetics Guanosine Tetraphosphate - metabolism Microbiology Molecular Sequence Data Prodigiosin - analogs & derivatives Prodigiosin - biosynthesis Promoter Regions, Genetic Streptomyces - genetics Streptomyces - growth & development Streptomyces - metabolism Streptomyces coelicolor Trans-Activators - genetics Transcription, Genetic |
| title | Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2) |
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