The effects of Hyaluronic acid, epidermal growth factor, and mitomycin in an experimental model of acute traumatic tympanic membrane perforation
The goal of this study was to evaluate the effects of hyaluronic acid, epidermal growth factor, and mitomycin C on the healing of acute experimental traumatic perforations of the tympanic membrane. Most acute perforations of the tympanic membrane heal spontaneously. However, some form of surgical tr...
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| Veröffentlicht in: | Otology & neurotology 2003-05, Vol.24 (3), p.371-376 |
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| creator | GÜNERI, Enis Alpin TEKIN, Selma YILMAZ, Osman ÖZKARA, Esra ERDAG, Taner Kemal IKIZ, Ahmet Ömer SARIOGLU, Sülen GÜNERI, Ataman |
| description | The goal of this study was to evaluate the effects of hyaluronic acid, epidermal growth factor, and mitomycin C on the healing of acute experimental traumatic perforations of the tympanic membrane.
Most acute perforations of the tympanic membrane heal spontaneously. However, some form of surgical treatment (i.e., myringoplasty) is needed for nonhealing perforations. Because the closure occurs by squamous epithelial migration, drugs that stimulate this regenerative process may aid in the closure of the perforation, obviating the need for more extensive treatments.
Bilateral perforations of the tympanic membrane were created in 30 rats, divided into three groups (A, B, C). The perforations in the right ears were treated with hyaluronic acid, epidermal growth factor, or mitomycin C. Those in the left ears were left untreated for comparison. To examine the healing process in different periods, 5 animals were killed in each group at days 3, 5, 7, 9 and 14. The other 5 animals in each group were observed daily to determine the duration of perforation closures. Thirty surgical specimens (5 right sides from each group and all 15 left sides in all groups) were histopathologically examined for tympanic membrane thickness, fibroblastic reaction, neovascularization, and crust morphology.
Hyaluronic acid and epidermal growth factor applications significantly shortened the healing in acute experimental traumatic perforations of the tympanic membrane (p = 0.0432); however, the difference between them was not significant (p = 0.3160). On the other side, tympanic membrane perforations treated with topical mitomycin C showed no evidence of closure. There were no significant differences in the histologic parameters between the treated groups and their contralateral control ears.
Hyaluronic acid and epidermal growth factor accelerated the closure of acute tympanic membrane perforations in rats. This may make them clinically useful in augmenting the efficiency of conservative treatments of acute perforations of the tympanic membrane. |
| doi_str_mv | 10.1097/00129492-200305000-00004 |
| format | Article |
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Most acute perforations of the tympanic membrane heal spontaneously. However, some form of surgical treatment (i.e., myringoplasty) is needed for nonhealing perforations. Because the closure occurs by squamous epithelial migration, drugs that stimulate this regenerative process may aid in the closure of the perforation, obviating the need for more extensive treatments.
Bilateral perforations of the tympanic membrane were created in 30 rats, divided into three groups (A, B, C). The perforations in the right ears were treated with hyaluronic acid, epidermal growth factor, or mitomycin C. Those in the left ears were left untreated for comparison. To examine the healing process in different periods, 5 animals were killed in each group at days 3, 5, 7, 9 and 14. The other 5 animals in each group were observed daily to determine the duration of perforation closures. Thirty surgical specimens (5 right sides from each group and all 15 left sides in all groups) were histopathologically examined for tympanic membrane thickness, fibroblastic reaction, neovascularization, and crust morphology.
Hyaluronic acid and epidermal growth factor applications significantly shortened the healing in acute experimental traumatic perforations of the tympanic membrane (p = 0.0432); however, the difference between them was not significant (p = 0.3160). On the other side, tympanic membrane perforations treated with topical mitomycin C showed no evidence of closure. There were no significant differences in the histologic parameters between the treated groups and their contralateral control ears.
Hyaluronic acid and epidermal growth factor accelerated the closure of acute tympanic membrane perforations in rats. This may make them clinically useful in augmenting the efficiency of conservative treatments of acute perforations of the tympanic membrane.</description><identifier>ISSN: 1531-7129</identifier><identifier>EISSN: 1537-4505</identifier><identifier>DOI: 10.1097/00129492-200305000-00004</identifier><identifier>PMID: 12806286</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Adjuvants, Immunologic - pharmacology ; Adjuvants, Immunologic - therapeutic use ; Animals ; Anti-Ulcer Agents - pharmacology ; Anti-Ulcer Agents - therapeutic use ; Antibiotics, Antineoplastic - pharmacology ; Biological and medical sciences ; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology ; Epidermal Growth Factor - pharmacology ; Epidermal Growth Factor - therapeutic use ; Fibroblasts - drug effects ; Hyaluronic Acid - pharmacology ; Hyaluronic Acid - therapeutic use ; Medical sciences ; Mitomycin - pharmacology ; Non tumoral diseases ; Otorhinolaryngology. Stomatology ; Random Allocation ; Rats ; Regeneration - drug effects ; Regeneration - physiology ; Tympanic Membrane - blood supply ; Tympanic Membrane - drug effects ; Tympanic Membrane - physiology ; Tympanic Membrane Perforation - drug therapy</subject><ispartof>Otology & neurotology, 2003-05, Vol.24 (3), p.371-376</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-c7f9407025d4cb9dfbd8b893872bc649969e1f5dd95d172d45d7786dd865d4153</citedby><cites>FETCH-LOGICAL-c341t-c7f9407025d4cb9dfbd8b893872bc649969e1f5dd95d172d45d7786dd865d4153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14844692$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12806286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GÜNERI, Enis Alpin</creatorcontrib><creatorcontrib>TEKIN, Selma</creatorcontrib><creatorcontrib>YILMAZ, Osman</creatorcontrib><creatorcontrib>ÖZKARA, Esra</creatorcontrib><creatorcontrib>ERDAG, Taner Kemal</creatorcontrib><creatorcontrib>IKIZ, Ahmet Ömer</creatorcontrib><creatorcontrib>SARIOGLU, Sülen</creatorcontrib><creatorcontrib>GÜNERI, Ataman</creatorcontrib><title>The effects of Hyaluronic acid, epidermal growth factor, and mitomycin in an experimental model of acute traumatic tympanic membrane perforation</title><title>Otology & neurotology</title><addtitle>Otol Neurotol</addtitle><description>The goal of this study was to evaluate the effects of hyaluronic acid, epidermal growth factor, and mitomycin C on the healing of acute experimental traumatic perforations of the tympanic membrane.
Most acute perforations of the tympanic membrane heal spontaneously. However, some form of surgical treatment (i.e., myringoplasty) is needed for nonhealing perforations. Because the closure occurs by squamous epithelial migration, drugs that stimulate this regenerative process may aid in the closure of the perforation, obviating the need for more extensive treatments.
Bilateral perforations of the tympanic membrane were created in 30 rats, divided into three groups (A, B, C). The perforations in the right ears were treated with hyaluronic acid, epidermal growth factor, or mitomycin C. Those in the left ears were left untreated for comparison. To examine the healing process in different periods, 5 animals were killed in each group at days 3, 5, 7, 9 and 14. The other 5 animals in each group were observed daily to determine the duration of perforation closures. Thirty surgical specimens (5 right sides from each group and all 15 left sides in all groups) were histopathologically examined for tympanic membrane thickness, fibroblastic reaction, neovascularization, and crust morphology.
Hyaluronic acid and epidermal growth factor applications significantly shortened the healing in acute experimental traumatic perforations of the tympanic membrane (p = 0.0432); however, the difference between them was not significant (p = 0.3160). On the other side, tympanic membrane perforations treated with topical mitomycin C showed no evidence of closure. There were no significant differences in the histologic parameters between the treated groups and their contralateral control ears.
Hyaluronic acid and epidermal growth factor accelerated the closure of acute tympanic membrane perforations in rats. This may make them clinically useful in augmenting the efficiency of conservative treatments of acute perforations of the tympanic membrane.</description><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Adjuvants, Immunologic - therapeutic use</subject><subject>Animals</subject><subject>Anti-Ulcer Agents - pharmacology</subject><subject>Anti-Ulcer Agents - therapeutic use</subject><subject>Antibiotics, Antineoplastic - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Epidermal Growth Factor - therapeutic use</subject><subject>Fibroblasts - drug effects</subject><subject>Hyaluronic Acid - pharmacology</subject><subject>Hyaluronic Acid - therapeutic use</subject><subject>Medical sciences</subject><subject>Mitomycin - pharmacology</subject><subject>Non tumoral diseases</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Regeneration - drug effects</subject><subject>Regeneration - physiology</subject><subject>Tympanic Membrane - blood supply</subject><subject>Tympanic Membrane - drug effects</subject><subject>Tympanic Membrane - physiology</subject><subject>Tympanic Membrane Perforation - drug therapy</subject><issn>1531-7129</issn><issn>1537-4505</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkd1uFSEUhYnR2Fp9BcONXnWU_59L06g1aeJNvZ4wsLFjhmEEJnrewkeW0x5tAmEnfGtt2AshTMk7Sqx-TwhlVlg2MEI4kYSQoW8inqBzKrkehCTy6X1NB93RM_Si1h9dpbnUz9EZZYYoZtQ5-nN7BxhiBN8qzhFfH9yyl7zOHjs_h0sM2xygJLfg7yX_anc4Ot9yucRuDTjNLaeDn1fcl1sx_N6gzAnW1vmUAyxHT-f3BrgVtyfXunE7pM0dOyRIU3Er4K6KufTLvL5Ez6JbKrw6nRfo26ePt1fXw83Xz1-uPtwMngvaBq-jFUQTJoPwkw1xCmYylhvNJq-EtcoCjTIEKwPVLAgZtDYqBKO6og_mAr198N1K_rlDbWOaq4dl6e_Jex0151xRrjpoHkBfcq0F4rj1L7pyGCkZj2mM_9IY_6cx3qfRpa9PPfYpQXgUnsbfgTcnwFXvltiH4ef6yAkjhLKM_wWYqZSB</recordid><startdate>20030501</startdate><enddate>20030501</enddate><creator>GÜNERI, Enis Alpin</creator><creator>TEKIN, Selma</creator><creator>YILMAZ, Osman</creator><creator>ÖZKARA, Esra</creator><creator>ERDAG, Taner Kemal</creator><creator>IKIZ, Ahmet Ömer</creator><creator>SARIOGLU, Sülen</creator><creator>GÜNERI, Ataman</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>20030501</creationdate><title>The effects of Hyaluronic acid, epidermal growth factor, and mitomycin in an experimental model of acute traumatic tympanic membrane perforation</title><author>GÜNERI, Enis Alpin ; TEKIN, Selma ; YILMAZ, Osman ; ÖZKARA, Esra ; ERDAG, Taner Kemal ; IKIZ, Ahmet Ömer ; SARIOGLU, Sülen ; GÜNERI, Ataman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-c7f9407025d4cb9dfbd8b893872bc649969e1f5dd95d172d45d7786dd865d4153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Adjuvants, Immunologic - therapeutic use</topic><topic>Animals</topic><topic>Anti-Ulcer Agents - pharmacology</topic><topic>Anti-Ulcer Agents - therapeutic use</topic><topic>Antibiotics, Antineoplastic - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Epidermal Growth Factor - therapeutic use</topic><topic>Fibroblasts - drug effects</topic><topic>Hyaluronic Acid - pharmacology</topic><topic>Hyaluronic Acid - therapeutic use</topic><topic>Medical sciences</topic><topic>Mitomycin - pharmacology</topic><topic>Non tumoral diseases</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Regeneration - drug effects</topic><topic>Regeneration - physiology</topic><topic>Tympanic Membrane - blood supply</topic><topic>Tympanic Membrane - drug effects</topic><topic>Tympanic Membrane - physiology</topic><topic>Tympanic Membrane Perforation - drug therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GÜNERI, Enis Alpin</creatorcontrib><creatorcontrib>TEKIN, Selma</creatorcontrib><creatorcontrib>YILMAZ, Osman</creatorcontrib><creatorcontrib>ÖZKARA, Esra</creatorcontrib><creatorcontrib>ERDAG, Taner Kemal</creatorcontrib><creatorcontrib>IKIZ, Ahmet Ömer</creatorcontrib><creatorcontrib>SARIOGLU, Sülen</creatorcontrib><creatorcontrib>GÜNERI, Ataman</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Otology & neurotology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GÜNERI, Enis Alpin</au><au>TEKIN, Selma</au><au>YILMAZ, Osman</au><au>ÖZKARA, Esra</au><au>ERDAG, Taner Kemal</au><au>IKIZ, Ahmet Ömer</au><au>SARIOGLU, Sülen</au><au>GÜNERI, Ataman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of Hyaluronic acid, epidermal growth factor, and mitomycin in an experimental model of acute traumatic tympanic membrane perforation</atitle><jtitle>Otology & neurotology</jtitle><addtitle>Otol Neurotol</addtitle><date>2003-05-01</date><risdate>2003</risdate><volume>24</volume><issue>3</issue><spage>371</spage><epage>376</epage><pages>371-376</pages><issn>1531-7129</issn><eissn>1537-4505</eissn><abstract>The goal of this study was to evaluate the effects of hyaluronic acid, epidermal growth factor, and mitomycin C on the healing of acute experimental traumatic perforations of the tympanic membrane.
Most acute perforations of the tympanic membrane heal spontaneously. However, some form of surgical treatment (i.e., myringoplasty) is needed for nonhealing perforations. Because the closure occurs by squamous epithelial migration, drugs that stimulate this regenerative process may aid in the closure of the perforation, obviating the need for more extensive treatments.
Bilateral perforations of the tympanic membrane were created in 30 rats, divided into three groups (A, B, C). The perforations in the right ears were treated with hyaluronic acid, epidermal growth factor, or mitomycin C. Those in the left ears were left untreated for comparison. To examine the healing process in different periods, 5 animals were killed in each group at days 3, 5, 7, 9 and 14. The other 5 animals in each group were observed daily to determine the duration of perforation closures. Thirty surgical specimens (5 right sides from each group and all 15 left sides in all groups) were histopathologically examined for tympanic membrane thickness, fibroblastic reaction, neovascularization, and crust morphology.
Hyaluronic acid and epidermal growth factor applications significantly shortened the healing in acute experimental traumatic perforations of the tympanic membrane (p = 0.0432); however, the difference between them was not significant (p = 0.3160). On the other side, tympanic membrane perforations treated with topical mitomycin C showed no evidence of closure. There were no significant differences in the histologic parameters between the treated groups and their contralateral control ears.
Hyaluronic acid and epidermal growth factor accelerated the closure of acute tympanic membrane perforations in rats. This may make them clinically useful in augmenting the efficiency of conservative treatments of acute perforations of the tympanic membrane.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>12806286</pmid><doi>10.1097/00129492-200305000-00004</doi><tpages>6</tpages></addata></record> |
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| ispartof | Otology & neurotology, 2003-05, Vol.24 (3), p.371-376 |
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| language | eng |
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| subjects | Adjuvants, Immunologic - pharmacology Adjuvants, Immunologic - therapeutic use Animals Anti-Ulcer Agents - pharmacology Anti-Ulcer Agents - therapeutic use Antibiotics, Antineoplastic - pharmacology Biological and medical sciences Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology Epidermal Growth Factor - pharmacology Epidermal Growth Factor - therapeutic use Fibroblasts - drug effects Hyaluronic Acid - pharmacology Hyaluronic Acid - therapeutic use Medical sciences Mitomycin - pharmacology Non tumoral diseases Otorhinolaryngology. Stomatology Random Allocation Rats Regeneration - drug effects Regeneration - physiology Tympanic Membrane - blood supply Tympanic Membrane - drug effects Tympanic Membrane - physiology Tympanic Membrane Perforation - drug therapy |
| title | The effects of Hyaluronic acid, epidermal growth factor, and mitomycin in an experimental model of acute traumatic tympanic membrane perforation |
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