Expression profiling of cell cycle regulatory proteins in oropharyngeal carcinomas using tissue microarrays
The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis. Paraffin blocks from 21 primary tumors were obt...
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| Veröffentlicht in: | In vivo (Athens) 2010-05, Vol.24 (3), p.315-320 |
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| container_title | In vivo (Athens) |
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| creator | Ribeiro, Daniel A Nascimento, Fabio D Fracalossi, Ana Carolina C Gomes, Thiago S Oshima, Celina T F Franco, Marcello F |
| description | The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis.
Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays.
Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx.
Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry. |
| format | Article |
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Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays.
Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx.
Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry.</description><identifier>ISSN: 0258-851X</identifier><identifier>PMID: 20555005</identifier><language>eng</language><publisher>Greece</publisher><subject>Biomarkers, Tumor - metabolism ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Cell Cycle Proteins - metabolism ; Cyclin-Dependent Kinase Inhibitor p16 - metabolism ; Cyclin-Dependent Kinase Inhibitor p21 - metabolism ; Humans ; Immunohistochemistry ; Oropharyngeal Neoplasms - metabolism ; Oropharyngeal Neoplasms - pathology ; Prognosis ; Protein Array Analysis ; Retinoblastoma Protein - metabolism ; Severity of Illness Index ; Tumor Suppressor Protein p53 - metabolism</subject><ispartof>In vivo (Athens), 2010-05, Vol.24 (3), p.315-320</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20555005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ribeiro, Daniel A</creatorcontrib><creatorcontrib>Nascimento, Fabio D</creatorcontrib><creatorcontrib>Fracalossi, Ana Carolina C</creatorcontrib><creatorcontrib>Gomes, Thiago S</creatorcontrib><creatorcontrib>Oshima, Celina T F</creatorcontrib><creatorcontrib>Franco, Marcello F</creatorcontrib><title>Expression profiling of cell cycle regulatory proteins in oropharyngeal carcinomas using tissue microarrays</title><title>In vivo (Athens)</title><addtitle>In Vivo</addtitle><description>The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis.
Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays.
Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx.
Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry.</description><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Oropharyngeal Neoplasms - metabolism</subject><subject>Oropharyngeal Neoplasms - pathology</subject><subject>Prognosis</subject><subject>Protein Array Analysis</subject><subject>Retinoblastoma Protein - metabolism</subject><subject>Severity of Illness Index</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><issn>0258-851X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1LxDAYhHNQ3HX1L0hungpp0rcfR1nWD1jwouCtpOnbGm2TmrcF--9t2fU0MDwMM3PBtkJCHuUQf2zYNdGXEGkmhLxiGykAQAjYsu_D7xCQyHrHh-Ab21nXct9wg13HzWw65AHbqdOjD_OKjGgdceu4D3741GF2LeoF1cFY53tNfKI1Y7REE_LemuB1CHqmG3bZ6I7w9qw79v54eNs_R8fXp5f9wzEaZCzGqFJJbApdVFmssUgrSABqJU2x-E0sTV6nGeSYJHUTV1I1WKHOwQgwkIvapGrH7k-5S9ufCWkse0vrHu3QT1RmSilQIhMLeXcmp6rHuhyC7ZdF5f8_6g9JIWOq</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Ribeiro, Daniel A</creator><creator>Nascimento, Fabio D</creator><creator>Fracalossi, Ana Carolina C</creator><creator>Gomes, Thiago S</creator><creator>Oshima, Celina T F</creator><creator>Franco, Marcello F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20100501</creationdate><title>Expression profiling of cell cycle regulatory proteins in oropharyngeal carcinomas using tissue microarrays</title><author>Ribeiro, Daniel A ; Nascimento, Fabio D ; Fracalossi, Ana Carolina C ; Gomes, Thiago S ; Oshima, Celina T F ; Franco, Marcello F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p210t-b341c9a9b71ae96b5455d32c9341f12c8d6758e44df1b23febea85c05c580dc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biomarkers, Tumor - metabolism</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Oropharyngeal Neoplasms - metabolism</topic><topic>Oropharyngeal Neoplasms - pathology</topic><topic>Prognosis</topic><topic>Protein Array Analysis</topic><topic>Retinoblastoma Protein - metabolism</topic><topic>Severity of Illness Index</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ribeiro, Daniel A</creatorcontrib><creatorcontrib>Nascimento, Fabio D</creatorcontrib><creatorcontrib>Fracalossi, Ana Carolina C</creatorcontrib><creatorcontrib>Gomes, Thiago S</creatorcontrib><creatorcontrib>Oshima, Celina T F</creatorcontrib><creatorcontrib>Franco, Marcello F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>In vivo (Athens)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ribeiro, Daniel A</au><au>Nascimento, Fabio D</au><au>Fracalossi, Ana Carolina C</au><au>Gomes, Thiago S</au><au>Oshima, Celina T F</au><au>Franco, Marcello F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression profiling of cell cycle regulatory proteins in oropharyngeal carcinomas using tissue microarrays</atitle><jtitle>In vivo (Athens)</jtitle><addtitle>In Vivo</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>24</volume><issue>3</issue><spage>315</spage><epage>320</epage><pages>315-320</pages><issn>0258-851X</issn><abstract>The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis.
Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays.
Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx.
Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry.</abstract><cop>Greece</cop><pmid>20555005</pmid><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biomarkers, Tumor - metabolism Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Cycle Proteins - metabolism Cyclin-Dependent Kinase Inhibitor p16 - metabolism Cyclin-Dependent Kinase Inhibitor p21 - metabolism Humans Immunohistochemistry Oropharyngeal Neoplasms - metabolism Oropharyngeal Neoplasms - pathology Prognosis Protein Array Analysis Retinoblastoma Protein - metabolism Severity of Illness Index Tumor Suppressor Protein p53 - metabolism |
| title | Expression profiling of cell cycle regulatory proteins in oropharyngeal carcinomas using tissue microarrays |
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