Therapeutic Angiogenesis by Implantation of a Capillary Structure Constituted of Human Adipose Tissue Microvascular Endothelial Cells

OBJECTIVE—We previously reported a novel technology for the engineering of a capillary network using an optical lithographic technique. To apply this technology to the therapy of ischemic diseases, we tested human omental microvascular endothelial cells (HOMECs) as an autologous cell source and dece...

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Veröffentlicht in:	Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2010-07, Vol.30 (7), p.1300-1306
Hauptverfasser:	Yoshida, Tomoko, Komaki, Motohiro, Hattori, Hideshi, Negishi, Jun, Kishida, Akio, Morita, Ikuo, Abe, Mayumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipose Tissue - blood supply Amnion - transplantation Animals Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Blood vessels and receptors Blotting, Western Cardiology. Vascular system Cell Movement Cells, Cultured Disease Models, Animal Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelial Cells - transplantation Fundamental and applied biological sciences. Psychology Hindlimb Humans Ischemia - physiopathology Ischemia - surgery Lysophospholipids - metabolism Male Medical sciences Mice Mice, Inbred BALB C Mice, Nude Microvessels - cytology Microvessels - drug effects Microvessels - metabolism Microvessels - transplantation Muscle, Skeletal - blood supply Neovascularization, Physiologic - drug effects Omentum - blood supply Protein Kinase Inhibitors - pharmacology Receptors, Lysosphingolipid - genetics Receptors, Lysosphingolipid - metabolism Regional Blood Flow Reverse Transcriptase Polymerase Chain Reaction rho GTP-Binding Proteins - metabolism rho-Associated Kinases - antagonists & inhibitors rho-Associated Kinases - metabolism Signal Transduction Sphingosine - analogs & derivatives Sphingosine - metabolism Tissue Engineering - methods Tissue Scaffolds Transplantation, Autologous Vertebrates: cardiovascular system
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