An Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Trypanosomal Antigens in Goat Serum Using a Monoclonal Antibody
An IgM murine monoclonal antibody (MAb) TEA 1/23.3.4.6 raised against circulating trypanosome antigens was used in a sandwich ELISA to assay trypanosomal antigens in a trypanosome lysate preparation and in sera from goats infected with Trypanosoma brucei evansi . As little as 1.25ug/ml of trypanosom...
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| Veröffentlicht in: | Journal of immunoassay (Monticello, N.Y.) N.Y.), 1992-06, Vol.13 (2), p.217-229 |
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| container_title | Journal of immunoassay (Monticello, N.Y.) |
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| creator | Olaho-Mukani, William Munyua, W. K. Njogu, A. R. |
| description | An IgM murine monoclonal antibody (MAb) TEA 1/23.3.4.6 raised against circulating trypanosome antigens was used in a sandwich ELISA to assay trypanosomal antigens in a trypanosome lysate preparation and in sera from goats infected with
Trypanosoma brucei evansi
. As little as 1.25ug/ml of trypanosomal antigen could be detected by this assay. Following infection, trypanosomal antigens were first detected in goat serum 24 hours after the intravenous (i/v) or 6 days after the intramuscular (i/m) inoculation of trypanosome parasites. Antigen levels remained detectable during the course of infection. After treatment with diminazene aceturate, antigens dropped to undetectable levels between day 12 to 41, suggesting that this assay offers a promising approach to the diagnosis of African Trypanosomiasis. |
| doi_str_mv | 10.1080/15321819208021228 |
| format | Article |
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Trypanosoma brucei evansi
. As little as 1.25ug/ml of trypanosomal antigen could be detected by this assay. Following infection, trypanosomal antigens were first detected in goat serum 24 hours after the intravenous (i/v) or 6 days after the intramuscular (i/m) inoculation of trypanosome parasites. Antigen levels remained detectable during the course of infection. After treatment with diminazene aceturate, antigens dropped to undetectable levels between day 12 to 41, suggesting that this assay offers a promising approach to the diagnosis of African Trypanosomiasis.</description><identifier>ISSN: 0197-1522</identifier><identifier>EISSN: 2332-4090</identifier><identifier>DOI: 10.1080/15321819208021228</identifier><identifier>PMID: 1430240</identifier><identifier>CODEN: JOUIDK</identifier><language>eng</language><publisher>Monticello, NY: Taylor & Francis Group</publisher><subject>African Trypanosomiasis ; Animals ; Antibodies, Monoclonal ; Antigens, Protozoan - blood ; Biological and medical sciences ; Diminazene - analogs & derivatives ; Diminazene - therapeutic use ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - statistics & numerical data ; Evaluation Studies as Topic ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Goats ; Immunoglobulin M ; Molecular immunology ; Monoclonal antibody ; Sensitivity and Specificity ; Techniques ; Trypanosoma brucei brucei - immunology ; Trypanosoma brucei evansi ; trypanosomal antigens ; Trypanosomiasis, African - diagnosis ; Trypanosomiasis, African - drug therapy ; Trypanosomiasis, African - immunology</subject><ispartof>Journal of immunoassay (Monticello, N.Y.), 1992-06, Vol.13 (2), p.217-229</ispartof><rights>Copyright Taylor & Francis Group, LLC 1992</rights><rights>1992 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-ec7bb698964464c64ab4a81c77a5a21ae21fb96ebf6983e0bd1921d47727c1843</citedby><cites>FETCH-LOGICAL-c378t-ec7bb698964464c64ab4a81c77a5a21ae21fb96ebf6983e0bd1921d47727c1843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/15321819208021228$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/15321819208021228$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,60409</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5374974$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1430240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olaho-Mukani, William</creatorcontrib><creatorcontrib>Munyua, W. K.</creatorcontrib><creatorcontrib>Njogu, A. R.</creatorcontrib><title>An Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Trypanosomal Antigens in Goat Serum Using a Monoclonal Antibody</title><title>Journal of immunoassay (Monticello, N.Y.)</title><addtitle>J Immunoassay</addtitle><description>An IgM murine monoclonal antibody (MAb) TEA 1/23.3.4.6 raised against circulating trypanosome antigens was used in a sandwich ELISA to assay trypanosomal antigens in a trypanosome lysate preparation and in sera from goats infected with
Trypanosoma brucei evansi
. As little as 1.25ug/ml of trypanosomal antigen could be detected by this assay. Following infection, trypanosomal antigens were first detected in goat serum 24 hours after the intravenous (i/v) or 6 days after the intramuscular (i/m) inoculation of trypanosome parasites. Antigen levels remained detectable during the course of infection. After treatment with diminazene aceturate, antigens dropped to undetectable levels between day 12 to 41, suggesting that this assay offers a promising approach to the diagnosis of African Trypanosomiasis.</description><subject>African Trypanosomiasis</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens, Protozoan - blood</subject><subject>Biological and medical sciences</subject><subject>Diminazene - analogs & derivatives</subject><subject>Diminazene - therapeutic use</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - statistics & numerical data</subject><subject>Evaluation Studies as Topic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Goats</subject><subject>Immunoglobulin M</subject><subject>Molecular immunology</subject><subject>Monoclonal antibody</subject><subject>Sensitivity and Specificity</subject><subject>Techniques</subject><subject>Trypanosoma brucei brucei - immunology</subject><subject>Trypanosoma brucei evansi</subject><subject>trypanosomal antigens</subject><subject>Trypanosomiasis, African - diagnosis</subject><subject>Trypanosomiasis, African - drug therapy</subject><subject>Trypanosomiasis, African - immunology</subject><issn>0197-1522</issn><issn>2332-4090</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2LFDEQhoMo67DuD_Ag5CDiHlrzNZ1u8NKs4zow4mF3z02Srl6jnWRM0kh7239uhhn1INalKOp5i-JB6DklbyhpyFu65ow2tGVlYJSx5hFaMc5ZJUhLHqMVoa2s6Jqxp-gipa-kVCuIaOozdEYFJ0yQFXroPN74n4uDamf9Nxjw1rnZhxSiBp9xl5Ja8OvNbnvTXeIxRJy_AH4PGUy2weMw4tu47NUh4dSEO5_tPfiErcfXQWV8A3F2-C5Zf48V_hR8MFPwJ1KHYXmGnoxqSnBx6ufo7sPm9upjtft8vb3qdpXhsskVGKl13TZtLUQtTC2UFqqhRkq1VowqYHTUbQ16LBAHoodihg5CSiYNbQQ_R6-Od_cxfJ8h5d7ZZGCalIcwp17y4k6SuoD0CJoYUoow9vtonYpLT0l_MN__Y75kXpyOz9rB8Ddx9Fz2L097lYyaxqi8sekPtuZStPLw47sjZn1R7dSPEKehz2qZQvyd4f__4hcPUpzD</recordid><startdate>19920601</startdate><enddate>19920601</enddate><creator>Olaho-Mukani, William</creator><creator>Munyua, W. K.</creator><creator>Njogu, A. R.</creator><general>Taylor & Francis Group</general><general>Dekker</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920601</creationdate><title>An Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Trypanosomal Antigens in Goat Serum Using a Monoclonal Antibody</title><author>Olaho-Mukani, William ; Munyua, W. K. ; Njogu, A. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-ec7bb698964464c64ab4a81c77a5a21ae21fb96ebf6983e0bd1921d47727c1843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>African Trypanosomiasis</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, Protozoan - blood</topic><topic>Biological and medical sciences</topic><topic>Diminazene - analogs & derivatives</topic><topic>Diminazene - therapeutic use</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - statistics & numerical data</topic><topic>Evaluation Studies as Topic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Goats</topic><topic>Immunoglobulin M</topic><topic>Molecular immunology</topic><topic>Monoclonal antibody</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><topic>Trypanosoma brucei brucei - immunology</topic><topic>Trypanosoma brucei evansi</topic><topic>trypanosomal antigens</topic><topic>Trypanosomiasis, African - diagnosis</topic><topic>Trypanosomiasis, African - drug therapy</topic><topic>Trypanosomiasis, African - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>Olaho-Mukani, William</creatorcontrib><creatorcontrib>Munyua, W. K.</creatorcontrib><creatorcontrib>Njogu, A. 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R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Trypanosomal Antigens in Goat Serum Using a Monoclonal Antibody</atitle><jtitle>Journal of immunoassay (Monticello, N.Y.)</jtitle><addtitle>J Immunoassay</addtitle><date>1992-06-01</date><risdate>1992</risdate><volume>13</volume><issue>2</issue><spage>217</spage><epage>229</epage><pages>217-229</pages><issn>0197-1522</issn><eissn>2332-4090</eissn><coden>JOUIDK</coden><abstract>An IgM murine monoclonal antibody (MAb) TEA 1/23.3.4.6 raised against circulating trypanosome antigens was used in a sandwich ELISA to assay trypanosomal antigens in a trypanosome lysate preparation and in sera from goats infected with
Trypanosoma brucei evansi
. As little as 1.25ug/ml of trypanosomal antigen could be detected by this assay. Following infection, trypanosomal antigens were first detected in goat serum 24 hours after the intravenous (i/v) or 6 days after the intramuscular (i/m) inoculation of trypanosome parasites. Antigen levels remained detectable during the course of infection. After treatment with diminazene aceturate, antigens dropped to undetectable levels between day 12 to 41, suggesting that this assay offers a promising approach to the diagnosis of African Trypanosomiasis.</abstract><cop>Monticello, NY</cop><pub>Taylor & Francis Group</pub><pmid>1430240</pmid><doi>10.1080/15321819208021228</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of immunoassay (Monticello, N.Y.), 1992-06, Vol.13 (2), p.217-229 |
| issn | 0197-1522 2332-4090 |
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| source | Taylor & Francis:Master (3349 titles); MEDLINE |
| subjects | African Trypanosomiasis Animals Antibodies, Monoclonal Antigens, Protozoan - blood Biological and medical sciences Diminazene - analogs & derivatives Diminazene - therapeutic use ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - statistics & numerical data Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Fundamental immunology Goats Immunoglobulin M Molecular immunology Monoclonal antibody Sensitivity and Specificity Techniques Trypanosoma brucei brucei - immunology Trypanosoma brucei evansi trypanosomal antigens Trypanosomiasis, African - diagnosis Trypanosomiasis, African - drug therapy Trypanosomiasis, African - immunology |
| title | An Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Trypanosomal Antigens in Goat Serum Using a Monoclonal Antibody |
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