An aptamer-based biosensor for mammalian initiation factor eukaryotic initiation factor 4A

Aptamers are short single-stranded DNA or RNA sequences that are selected in vitro based on their high affinity to a target molecule. Here we demonstrate that an RNA aptamer selected against eukaryotic initiation factor 4A (eIF4A) serves as an efficient biosensor. The aptamer, when immobilized to re...

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Veröffentlicht in:	Analytical biochemistry 2009-05, Vol.388 (1), p.102-107
Hauptverfasser:	Oguro, Akihiro, Ohtsu, Takashi, Nakamura, Yoshikazu
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity purification Aptamer Aptamers, Nucleotide - chemistry Biosensing Techniques - methods Biosensor Cell Line DNA, Single-Stranded - chemistry eIF4A Eukaryotic Initiation Factor-4A - chemistry Eukaryotic Initiation Factor-4A - isolation & purification Eukaryotic Initiation Factor-4A - metabolism HeLa Cells Humans Immunoblotting SPR
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