Production and synthetic dyes decolourization capacity of a recombinant laccase from Pichia pastoris

To produce and purify a recombinant laccase from Pichia pastoris and to test its ability in decolourization of synthetic dyes. A cDNA encoding for a laccase was isolated from Pycnoporus sanguineus and was expressed in P. pastoris strain SMD1168H under the control of the alcohol oxidase (AOX1) promot...

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Veröffentlicht in:	Journal of applied microbiology 2009-10, Vol.107 (4), p.1149-1156
Hauptverfasser:	Lu, L, Zhao, M, Liang, S.-C, Zhao, L.-Y, Li, D.-B, Zhang, B.-B
Format:	Artikel
Sprache:	eng
Schlagworte:	Biodegradation, Environmental Biological and medical sciences Cloning, Molecular Coloring Agents - chemistry Coloring Agents - metabolism DNA, Complementary - genetics dye decolourization Fundamental and applied biological sciences. Psychology heterologous production Hydrogen-Ion Concentration laccase Laccase - biosynthesis Laccase - genetics Laccase - metabolism Microbiology Pichia - enzymology Pichia - genetics Pichia pastoris Protein Engineering - methods purification Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Temperature Transformation, Genetic
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