On-Chip Aptamer-Based Sandwich Assay for Thrombin Detection Employing Magnetic Beads and Quantum Dots

In this paper, we report the development of an on-chip aptamer-based fluorescence assay for protein detection and quantification based on sandwich ELISA principles. Thrombin was selected as a model analyte to validate the assay design, which involves two DNA thrombin aptamers recognizing two differe...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Analytical chemistry (Washington) 2010-07, Vol.82 (13), p.5591-5597
Hauptverfasser:	Tennico, Yolanda H, Hutanu, Daniela, Koesdjojo, Myra T, Bartel, Cheryl Moody, Remcho, Vincent T
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Aptamers, Nucleotide - chemistry Chemistry Enzyme-Linked Immunosorbent Assay Exact sciences and technology Fluorescence Magnetics Microfluidic Analytical Techniques - methods Microscopy, Fluorescence - methods Miscellaneous Protein Binding Proteins Quantum Dots Thrombin - analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!