Production of a rat monoclonal antibody specific for Myf5
Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early...
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| Veröffentlicht in: | Hybridoma (2005) 2010-02, Vol.29 (1), p.59-62 |
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| container_title | Hybridoma (2005) |
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| creator | Harada, Akihito Okada, Seiji Odawara, Jun Kumamaru, Hiromi Saiwai, Hirokazu Aoki, Mayumi Nakamura, Mako Nishiyama, Yuko Ohkawa, Yasuyuki |
| description | Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function. |
| doi_str_mv | 10.1089/hyb.2009.0066 |
| format | Article |
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Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function.</description><identifier>ISSN: 1554-0014</identifier><identifier>EISSN: 1557-8348</identifier><identifier>DOI: 10.1089/hyb.2009.0066</identifier><identifier>PMID: 20199153</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - isolation & purification ; Antibody Specificity ; Blotting, Western ; Cell proliferation ; Differentiation ; Enzyme-Linked Immunosorbent Assay ; Female ; Genetic aspects ; Helix-loop-helix proteins ; Homology ; Hybridomas ; Hybridomas - metabolism ; Ileum - immunology ; Immunization ; Immunoenzyme Techniques ; Lymph nodes ; Lymph Nodes - cytology ; Lymph Nodes - immunology ; Mice ; Monoclonal antibodies ; Myf5 protein ; Myoblasts ; Myoblasts - cytology ; Myoblasts - immunology ; Myoblasts - metabolism ; MyoD protein ; Myogenic Regulatory Factor 5 - genetics ; Myogenic Regulatory Factor 5 - immunology ; myogenin ; NIH 3T3 Cells ; Physiological aspects ; Plasmids ; Production processes ; Rats ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Transcription factors</subject><ispartof>Hybridoma (2005), 2010-02, Vol.29 (1), p.59-62</ispartof><rights>COPYRIGHT 2010 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-5223a69ddf29693d0f9d73c7b2eec3b2157d1f7bdf5d45ccd612d35af1159cc33</citedby><cites>FETCH-LOGICAL-c458t-5223a69ddf29693d0f9d73c7b2eec3b2157d1f7bdf5d45ccd612d35af1159cc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20199153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harada, Akihito</creatorcontrib><creatorcontrib>Okada, Seiji</creatorcontrib><creatorcontrib>Odawara, Jun</creatorcontrib><creatorcontrib>Kumamaru, Hiromi</creatorcontrib><creatorcontrib>Saiwai, Hirokazu</creatorcontrib><creatorcontrib>Aoki, Mayumi</creatorcontrib><creatorcontrib>Nakamura, Mako</creatorcontrib><creatorcontrib>Nishiyama, Yuko</creatorcontrib><creatorcontrib>Ohkawa, Yasuyuki</creatorcontrib><title>Production of a rat monoclonal antibody specific for Myf5</title><title>Hybridoma (2005)</title><addtitle>Hybridoma (Larchmt)</addtitle><description>Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Antibody Specificity</subject><subject>Blotting, Western</subject><subject>Cell proliferation</subject><subject>Differentiation</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Helix-loop-helix proteins</subject><subject>Homology</subject><subject>Hybridomas</subject><subject>Hybridomas - metabolism</subject><subject>Ileum - immunology</subject><subject>Immunization</subject><subject>Immunoenzyme Techniques</subject><subject>Lymph nodes</subject><subject>Lymph Nodes - cytology</subject><subject>Lymph Nodes - immunology</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>Myf5 protein</subject><subject>Myoblasts</subject><subject>Myoblasts - cytology</subject><subject>Myoblasts - immunology</subject><subject>Myoblasts - metabolism</subject><subject>MyoD protein</subject><subject>Myogenic Regulatory Factor 5 - genetics</subject><subject>Myogenic Regulatory Factor 5 - immunology</subject><subject>myogenin</subject><subject>NIH 3T3 Cells</subject><subject>Physiological aspects</subject><subject>Plasmids</subject><subject>Production processes</subject><subject>Rats</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Transcription factors</subject><issn>1554-0014</issn><issn>1557-8348</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90ctLwzAcwPEgipvTo1cpeNBLZx5NkxzH8AUTPeg5pHlopG1m0h3639u6KQgiOSSEzy8EvgCcIjhHkIurt76aYwjFHMKy3ANTRCnLOSn4_te5yCFExQQcpfQOISk5ZodggiESAlEyBeIpBrPRnQ9tFlymsqi6rAlt0HVoVZ2ptvNVMH2W1lZ753XmQsweekePwYFTdbInu30GXm6un5d3-erx9n65WOW6oLzLKcZElcIYh0UpiIFOGEY0q7C1mlQYUWaQY5Vx1BRUa1MibAhVDiEqtCZkBi62765j-NjY1MnGJ23rWrU2bJJkhGDBGWGDvPxXIoIJR5zCkZ5v6auqrfStC11UeuRygTHkxQCLQc3_UMMytvE6tNb54f7XQL4d0DGkFK2T6-gbFXuJoBxzySGXHHPJMdfgz3Y_3lSNNT_6uw_5BKTXjaQ</recordid><startdate>201002</startdate><enddate>201002</enddate><creator>Harada, Akihito</creator><creator>Okada, Seiji</creator><creator>Odawara, Jun</creator><creator>Kumamaru, Hiromi</creator><creator>Saiwai, Hirokazu</creator><creator>Aoki, Mayumi</creator><creator>Nakamura, Mako</creator><creator>Nishiyama, Yuko</creator><creator>Ohkawa, Yasuyuki</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>201002</creationdate><title>Production of a rat monoclonal antibody specific for Myf5</title><author>Harada, Akihito ; Okada, Seiji ; Odawara, Jun ; Kumamaru, Hiromi ; Saiwai, Hirokazu ; Aoki, Mayumi ; Nakamura, Mako ; Nishiyama, Yuko ; Ohkawa, Yasuyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-5223a69ddf29693d0f9d73c7b2eec3b2157d1f7bdf5d45ccd612d35af1159cc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - isolation & purification</topic><topic>Antibody Specificity</topic><topic>Blotting, Western</topic><topic>Cell proliferation</topic><topic>Differentiation</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Helix-loop-helix proteins</topic><topic>Homology</topic><topic>Hybridomas</topic><topic>Hybridomas - metabolism</topic><topic>Ileum - immunology</topic><topic>Immunization</topic><topic>Immunoenzyme Techniques</topic><topic>Lymph nodes</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - immunology</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>Myf5 protein</topic><topic>Myoblasts</topic><topic>Myoblasts - cytology</topic><topic>Myoblasts - immunology</topic><topic>Myoblasts - metabolism</topic><topic>MyoD protein</topic><topic>Myogenic Regulatory Factor 5 - genetics</topic><topic>Myogenic Regulatory Factor 5 - immunology</topic><topic>myogenin</topic><topic>NIH 3T3 Cells</topic><topic>Physiological aspects</topic><topic>Plasmids</topic><topic>Production processes</topic><topic>Rats</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harada, Akihito</creatorcontrib><creatorcontrib>Okada, Seiji</creatorcontrib><creatorcontrib>Odawara, Jun</creatorcontrib><creatorcontrib>Kumamaru, Hiromi</creatorcontrib><creatorcontrib>Saiwai, Hirokazu</creatorcontrib><creatorcontrib>Aoki, Mayumi</creatorcontrib><creatorcontrib>Nakamura, Mako</creatorcontrib><creatorcontrib>Nishiyama, Yuko</creatorcontrib><creatorcontrib>Ohkawa, Yasuyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Hybridoma (2005)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harada, Akihito</au><au>Okada, Seiji</au><au>Odawara, Jun</au><au>Kumamaru, Hiromi</au><au>Saiwai, Hirokazu</au><au>Aoki, Mayumi</au><au>Nakamura, Mako</au><au>Nishiyama, Yuko</au><au>Ohkawa, Yasuyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of a rat monoclonal antibody specific for Myf5</atitle><jtitle>Hybridoma (2005)</jtitle><addtitle>Hybridoma (Larchmt)</addtitle><date>2010-02</date><risdate>2010</risdate><volume>29</volume><issue>1</issue><spage>59</spage><epage>62</epage><pages>59-62</pages><issn>1554-0014</issn><eissn>1557-8348</eissn><abstract>Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>20199153</pmid><doi>10.1089/hyb.2009.0066</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Antibody Specificity Blotting, Western Cell proliferation Differentiation Enzyme-Linked Immunosorbent Assay Female Genetic aspects Helix-loop-helix proteins Homology Hybridomas Hybridomas - metabolism Ileum - immunology Immunization Immunoenzyme Techniques Lymph nodes Lymph Nodes - cytology Lymph Nodes - immunology Mice Monoclonal antibodies Myf5 protein Myoblasts Myoblasts - cytology Myoblasts - immunology Myoblasts - metabolism MyoD protein Myogenic Regulatory Factor 5 - genetics Myogenic Regulatory Factor 5 - immunology myogenin NIH 3T3 Cells Physiological aspects Plasmids Production processes Rats Recombinant Proteins - genetics Recombinant Proteins - immunology Transcription factors |
| title | Production of a rat monoclonal antibody specific for Myf5 |
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