Preliminary study on somatic cell nuclear transfer in rabbits in Thailand
The objective of the study was to develop the somatic nuclear transfer technique by using rabbits as the model. The oocyte recipients aged 16 h post coitus were collected surgically from 20 superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with a vasectomized...
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| Veröffentlicht in: | Journal of the Medical Association of Thailand 2003-03, Vol.86 (3), p.276-281 |
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| creator | TECHAKUMPHU, Mongkol SUWAJANAKORN, Somchai BOONKASEMSANTI, Wisut VIRUTAMASEN, Pramuan NUMCHAISRIKA, Pranee PRUKSANANONDA, Kamtorn AHNONKITPANIT, Vichuda |
| description | The objective of the study was to develop the somatic nuclear transfer technique by using rabbits as the model. The oocyte recipients aged 16 h post coitus were collected surgically from 20 superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with a vasectomized male. The metaphase II plate and 1st polar body of oocyte was later aspirated by enucleated micropipette under an inverted microscope. A single donor cell; cumulus cell or cultured or frozen fibroblast cell from passage 1 to 9 were transferred to enucleated oocyte and fused with triple DC pulses, 3.2 kv, 20 micros. The fused embryos were cultivated in TCM 199 NaHCO3 + 10 per cent fetal calf serum (FCS) for 4 days. The cleavage rate (2-cell stage) was 37.2 per cent (32/86) from eight experiments, and 18.8 per cent (6/32) developed to the early morula stage. This study also indicated that the enucleation pipette and the somatic cell type influenced the success. |
| format | Article |
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The oocyte recipients aged 16 h post coitus were collected surgically from 20 superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with a vasectomized male. The metaphase II plate and 1st polar body of oocyte was later aspirated by enucleated micropipette under an inverted microscope. A single donor cell; cumulus cell or cultured or frozen fibroblast cell from passage 1 to 9 were transferred to enucleated oocyte and fused with triple DC pulses, 3.2 kv, 20 micros. The fused embryos were cultivated in TCM 199 NaHCO3 + 10 per cent fetal calf serum (FCS) for 4 days. The cleavage rate (2-cell stage) was 37.2 per cent (32/86) from eight experiments, and 18.8 per cent (6/32) developed to the early morula stage. This study also indicated that the enucleation pipette and the somatic cell type influenced the success.</description><identifier>ISSN: 0125-2208</identifier><identifier>PMID: 12757069</identifier><identifier>CODEN: JMTHBU</identifier><language>eng</language><publisher>Bangkok: Medical Association of Thailand</publisher><subject>Active Transport, Cell Nucleus ; Animals ; Biological and medical sciences ; Birth control ; Cloning, Organism - methods ; Cloning, Organism - veterinary ; Coculture Techniques ; Embryonic and Fetal Development - physiology ; Female ; Fibroblasts - cytology ; Gynecology. Andrology. Obstetrics ; Male ; Medical sciences ; Models, Animal ; Oocytes - growth & development ; Oocytes - transplantation ; Other methods of contraception. Sterilization ; Rabbits ; Sensitivity and Specificity ; Tropical medicine</subject><ispartof>Journal of the Medical Association of Thailand, 2003-03, Vol.86 (3), p.276-281</ispartof><rights>2004 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15115452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12757069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TECHAKUMPHU, Mongkol</creatorcontrib><creatorcontrib>SUWAJANAKORN, Somchai</creatorcontrib><creatorcontrib>BOONKASEMSANTI, Wisut</creatorcontrib><creatorcontrib>VIRUTAMASEN, Pramuan</creatorcontrib><creatorcontrib>NUMCHAISRIKA, Pranee</creatorcontrib><creatorcontrib>PRUKSANANONDA, Kamtorn</creatorcontrib><creatorcontrib>AHNONKITPANIT, Vichuda</creatorcontrib><title>Preliminary study on somatic cell nuclear transfer in rabbits in Thailand</title><title>Journal of the Medical Association of Thailand</title><addtitle>J Med Assoc Thai</addtitle><description>The objective of the study was to develop the somatic nuclear transfer technique by using rabbits as the model. The oocyte recipients aged 16 h post coitus were collected surgically from 20 superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with a vasectomized male. The metaphase II plate and 1st polar body of oocyte was later aspirated by enucleated micropipette under an inverted microscope. A single donor cell; cumulus cell or cultured or frozen fibroblast cell from passage 1 to 9 were transferred to enucleated oocyte and fused with triple DC pulses, 3.2 kv, 20 micros. The fused embryos were cultivated in TCM 199 NaHCO3 + 10 per cent fetal calf serum (FCS) for 4 days. The cleavage rate (2-cell stage) was 37.2 per cent (32/86) from eight experiments, and 18.8 per cent (6/32) developed to the early morula stage. This study also indicated that the enucleation pipette and the somatic cell type influenced the success.</description><subject>Active Transport, Cell Nucleus</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cloning, Organism - methods</subject><subject>Cloning, Organism - veterinary</subject><subject>Coculture Techniques</subject><subject>Embryonic and Fetal Development - physiology</subject><subject>Female</subject><subject>Fibroblasts - cytology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Models, Animal</subject><subject>Oocytes - growth & development</subject><subject>Oocytes - transplantation</subject><subject>Other methods of contraception. Sterilization</subject><subject>Rabbits</subject><subject>Sensitivity and Specificity</subject><subject>Tropical medicine</subject><issn>0125-2208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFzz1PwzAQgGEPIFoKfwF5gS2SPxN7RBUflSrBUObo7NjCyHGCnQz996SiiOlueHS69wKtCWWyYoyoFbou5YsQIXXNr9CKskY2pNZrtHvPLoY-JMhHXKa5O-Ih4TL0MAWLrYsRp9lGBxlPGVLxLuOQcAZjwlRO6-ETQoTU3aBLD7G42_PcoI_np8P2tdq_vey2j_tqpJJNlSCi9pxrZcBax4wxQhhDuTdKd5ppokGAIMorb6U1nXTEWmlI3TnqmkbzDXr4vTvm4Xt2ZWr7UE6PQnLDXNqGc6a5Ugu8O8PZ9K5rxxz6pbL9i1_A_RlAsRD90mdD-XeSUikk4z8CB2Km</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>TECHAKUMPHU, Mongkol</creator><creator>SUWAJANAKORN, Somchai</creator><creator>BOONKASEMSANTI, Wisut</creator><creator>VIRUTAMASEN, Pramuan</creator><creator>NUMCHAISRIKA, Pranee</creator><creator>PRUKSANANONDA, Kamtorn</creator><creator>AHNONKITPANIT, Vichuda</creator><general>Medical Association of Thailand</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Preliminary study on somatic cell nuclear transfer in rabbits in Thailand</title><author>TECHAKUMPHU, Mongkol ; SUWAJANAKORN, Somchai ; BOONKASEMSANTI, Wisut ; VIRUTAMASEN, Pramuan ; NUMCHAISRIKA, Pranee ; PRUKSANANONDA, Kamtorn ; AHNONKITPANIT, Vichuda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p152t-4046f3398bacce2bbb44bb13fb89d92909a4a408f8fc5cbd5e0cc5b06de1e7793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Active Transport, Cell Nucleus</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cloning, Organism - methods</topic><topic>Cloning, Organism - veterinary</topic><topic>Coculture Techniques</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Female</topic><topic>Fibroblasts - cytology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Models, Animal</topic><topic>Oocytes - growth & development</topic><topic>Oocytes - transplantation</topic><topic>Other methods of contraception. Sterilization</topic><topic>Rabbits</topic><topic>Sensitivity and Specificity</topic><topic>Tropical medicine</topic><toplevel>online_resources</toplevel><creatorcontrib>TECHAKUMPHU, Mongkol</creatorcontrib><creatorcontrib>SUWAJANAKORN, Somchai</creatorcontrib><creatorcontrib>BOONKASEMSANTI, Wisut</creatorcontrib><creatorcontrib>VIRUTAMASEN, Pramuan</creatorcontrib><creatorcontrib>NUMCHAISRIKA, Pranee</creatorcontrib><creatorcontrib>PRUKSANANONDA, Kamtorn</creatorcontrib><creatorcontrib>AHNONKITPANIT, Vichuda</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the Medical Association of Thailand</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TECHAKUMPHU, Mongkol</au><au>SUWAJANAKORN, Somchai</au><au>BOONKASEMSANTI, Wisut</au><au>VIRUTAMASEN, Pramuan</au><au>NUMCHAISRIKA, Pranee</au><au>PRUKSANANONDA, Kamtorn</au><au>AHNONKITPANIT, Vichuda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preliminary study on somatic cell nuclear transfer in rabbits in Thailand</atitle><jtitle>Journal of the Medical Association of Thailand</jtitle><addtitle>J Med Assoc Thai</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>86</volume><issue>3</issue><spage>276</spage><epage>281</epage><pages>276-281</pages><issn>0125-2208</issn><coden>JMTHBU</coden><abstract>The objective of the study was to develop the somatic nuclear transfer technique by using rabbits as the model. The oocyte recipients aged 16 h post coitus were collected surgically from 20 superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with a vasectomized male. The metaphase II plate and 1st polar body of oocyte was later aspirated by enucleated micropipette under an inverted microscope. A single donor cell; cumulus cell or cultured or frozen fibroblast cell from passage 1 to 9 were transferred to enucleated oocyte and fused with triple DC pulses, 3.2 kv, 20 micros. The fused embryos were cultivated in TCM 199 NaHCO3 + 10 per cent fetal calf serum (FCS) for 4 days. The cleavage rate (2-cell stage) was 37.2 per cent (32/86) from eight experiments, and 18.8 per cent (6/32) developed to the early morula stage. This study also indicated that the enucleation pipette and the somatic cell type influenced the success.</abstract><cop>Bangkok</cop><pub>Medical Association of Thailand</pub><pmid>12757069</pmid><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Active Transport, Cell Nucleus Animals Biological and medical sciences Birth control Cloning, Organism - methods Cloning, Organism - veterinary Coculture Techniques Embryonic and Fetal Development - physiology Female Fibroblasts - cytology Gynecology. Andrology. Obstetrics Male Medical sciences Models, Animal Oocytes - growth & development Oocytes - transplantation Other methods of contraception. Sterilization Rabbits Sensitivity and Specificity Tropical medicine |
| title | Preliminary study on somatic cell nuclear transfer in rabbits in Thailand |
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