Approaching the Complexity of Elastase-Digested Membrane Proteomes Using Off-Gel IEF/nLC-MALDI-MS/MS

Liquid chromatography, coupled with tandem mass spectrometry, is an established method for the identification of proteins from a complex sample. Despite its wide application, the analysis of whole proteomes still represents a challenge to researchers, because of the complexity and dynamic range of p...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-03, Vol.82 (5), p.2145-2149
Hauptverfasser:	Arrey, Tabiwang N, Rietschel, Benjamin, Papasotiriou, Dimitrios G, Bornemann, Sandra, Baeumlisberger, Dominic, Karas, Michael, Meyer, Bjoern
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Bacterial Proteins - metabolism Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, Liquid - methods Corynebacterium glutamicum - metabolism Enzymes Exact sciences and technology Isoelectric Focusing - methods Mass spectrometry Membrane Proteins - metabolism Membranes Other chromatographic methods Pancreatic Elastase - metabolism Proteases Proteome Proteomics Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Tandem Mass Spectrometry - methods
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