Calcium requirements for human sperm function in vitro
To determine extracellular calcium (Ca 2+) requirements for the maintenance of human sperm function in vitro. Prospective study. Basic research laboratory. Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET. Sp...
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| Veröffentlicht in: | Fertility and sterility 2003-06, Vol.79 (6), p.1396-1403 |
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| container_title | Fertility and sterility |
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| creator | Marín-Briggiler, Clara I Gonzalez-Echeverría, Fernanda Buffone, Mariano Calamera, Juan C Tezón, Jorge G Vazquez-Levin, Mónica H |
| description | To determine extracellular calcium (Ca
2+) requirements for the maintenance of human sperm function in vitro.
Prospective study.
Basic research laboratory.
Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET.
Spermatozoa were incubated for ≤18 hours in media containing different CaCl
2 concentrations (maximum, 2.5 mM [control]).
Protein tyrosine phosphorylation patterns, development of hyperactivated motility, induction of the acrosome reaction (AR) in response to human FF, and sperm interaction with homologous zona pellucida (ZP).
Cells maintained for 18 hours in medium containing ≥0.1 mM of Ca
2+ were able to undergo the AR when exposed to human FF in the presence of 2.5 mM of Ca
2+. Calcium concentrations of ≥0.22 mM were sufficient to reach protein tyrosine phosphorylation levels and hyperactivated motility values similar to those of controls. Higher Ca
2+ concentrations (≥0.58 mM) were required to produce maximum human FF–induced AR in previously capacitated cells and to obtain an adequate sperm–ZP binding.
Different steps of the fertilization process have distinctive Ca
2+ requirements. Whereas 0.22 mM of Ca
2+ is sufficient for the development of some capacitation-related events, human FF–induced AR and sperm–ZP interaction require 0.58 mM of this cation. |
| doi_str_mv | 10.1016/S0015-0282(03)00267-X |
| format | Article |
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2+) requirements for the maintenance of human sperm function in vitro.
Prospective study.
Basic research laboratory.
Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET.
Spermatozoa were incubated for ≤18 hours in media containing different CaCl
2 concentrations (maximum, 2.5 mM [control]).
Protein tyrosine phosphorylation patterns, development of hyperactivated motility, induction of the acrosome reaction (AR) in response to human FF, and sperm interaction with homologous zona pellucida (ZP).
Cells maintained for 18 hours in medium containing ≥0.1 mM of Ca
2+ were able to undergo the AR when exposed to human FF in the presence of 2.5 mM of Ca
2+. Calcium concentrations of ≥0.22 mM were sufficient to reach protein tyrosine phosphorylation levels and hyperactivated motility values similar to those of controls. Higher Ca
2+ concentrations (≥0.58 mM) were required to produce maximum human FF–induced AR in previously capacitated cells and to obtain an adequate sperm–ZP binding.
Different steps of the fertilization process have distinctive Ca
2+ requirements. Whereas 0.22 mM of Ca
2+ is sufficient for the development of some capacitation-related events, human FF–induced AR and sperm–ZP interaction require 0.58 mM of this cation.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(03)00267-X</identifier><identifier>PMID: 12798888</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Acrosome - physiology ; Acrosome reaction ; Biological and medical sciences ; calcium ; Calcium - pharmacology ; capacitation ; Fundamental and applied biological sciences. Psychology ; human spermatozoa ; Humans ; In Vitro Techniques ; Male ; Mammalian male genital system ; Morphology. Physiology ; Prospective Studies ; Sperm Capacitation - physiology ; Sperm-Ovum Interactions ; Spermatozoa - physiology ; Vertebrates: reproduction ; zona pellucida ; Zona Pellucida - physiology</subject><ispartof>Fertility and sterility, 2003-06, Vol.79 (6), p.1396-1403</ispartof><rights>2003 American Society for Reproductive Medicine</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-cfdb25be7cf2ab886e3dc23136ca3879e428ddedefb0187203dcaf265238111e3</citedby><cites>FETCH-LOGICAL-c438t-cfdb25be7cf2ab886e3dc23136ca3879e428ddedefb0187203dcaf265238111e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(03)00267-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3548,23929,23930,25139,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14849611$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12798888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marín-Briggiler, Clara I</creatorcontrib><creatorcontrib>Gonzalez-Echeverría, Fernanda</creatorcontrib><creatorcontrib>Buffone, Mariano</creatorcontrib><creatorcontrib>Calamera, Juan C</creatorcontrib><creatorcontrib>Tezón, Jorge G</creatorcontrib><creatorcontrib>Vazquez-Levin, Mónica H</creatorcontrib><title>Calcium requirements for human sperm function in vitro</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To determine extracellular calcium (Ca
2+) requirements for the maintenance of human sperm function in vitro.
Prospective study.
Basic research laboratory.
Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET.
Spermatozoa were incubated for ≤18 hours in media containing different CaCl
2 concentrations (maximum, 2.5 mM [control]).
Protein tyrosine phosphorylation patterns, development of hyperactivated motility, induction of the acrosome reaction (AR) in response to human FF, and sperm interaction with homologous zona pellucida (ZP).
Cells maintained for 18 hours in medium containing ≥0.1 mM of Ca
2+ were able to undergo the AR when exposed to human FF in the presence of 2.5 mM of Ca
2+. Calcium concentrations of ≥0.22 mM were sufficient to reach protein tyrosine phosphorylation levels and hyperactivated motility values similar to those of controls. Higher Ca
2+ concentrations (≥0.58 mM) were required to produce maximum human FF–induced AR in previously capacitated cells and to obtain an adequate sperm–ZP binding.
Different steps of the fertilization process have distinctive Ca
2+ requirements. Whereas 0.22 mM of Ca
2+ is sufficient for the development of some capacitation-related events, human FF–induced AR and sperm–ZP interaction require 0.58 mM of this cation.</description><subject>Acrosome - physiology</subject><subject>Acrosome reaction</subject><subject>Biological and medical sciences</subject><subject>calcium</subject><subject>Calcium - pharmacology</subject><subject>capacitation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>human spermatozoa</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Prospective Studies</subject><subject>Sperm Capacitation - physiology</subject><subject>Sperm-Ovum Interactions</subject><subject>Spermatozoa - physiology</subject><subject>Vertebrates: reproduction</subject><subject>zona pellucida</subject><subject>Zona Pellucida - physiology</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotlZ_grIXRQ-r-dhk05NI8QsKHlToLWSzE4zsR5vsFvz3pu1ij85lDvO8M8OD0DnBtwQTcfeOMeEpppJeY3aDMRV5ujhAY8K5SLng7BCN_5AROgnhG2MsSE6P0YjQfCpjjZGY6cq4vk48rHrnoYamC4ltffLV17pJwhJ8ndi-MZ1rm8Q1ydp1vj1FR1ZXAc6GPkGfT48fs5d0_vb8OnuYpyZjskuNLQvKC8iNpbqQUgArDWWECaOZzKeQUVmWUIItMJE5xXGsLRWcMkkIATZBV7u9S9-uegidql0wUFW6gbYPKmeMTrHkEeQ70Pg2BA9WLb2rtf9RBKuNMLUVpjY2FGZqK0wtYu5iONAXNZT71GAoApcDoIPRlfW6MS7suUxmU0FI5O53HEQdawdeBeOgMVBGq6ZTZev-eeUX6wqHxg</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>Marín-Briggiler, Clara I</creator><creator>Gonzalez-Echeverría, Fernanda</creator><creator>Buffone, Mariano</creator><creator>Calamera, Juan C</creator><creator>Tezón, Jorge G</creator><creator>Vazquez-Levin, Mónica H</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>Calcium requirements for human sperm function in vitro</title><author>Marín-Briggiler, Clara I ; Gonzalez-Echeverría, Fernanda ; Buffone, Mariano ; Calamera, Juan C ; Tezón, Jorge G ; Vazquez-Levin, Mónica H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-cfdb25be7cf2ab886e3dc23136ca3879e428ddedefb0187203dcaf265238111e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Acrosome - physiology</topic><topic>Acrosome reaction</topic><topic>Biological and medical sciences</topic><topic>calcium</topic><topic>Calcium - pharmacology</topic><topic>capacitation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>human spermatozoa</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Prospective Studies</topic><topic>Sperm Capacitation - physiology</topic><topic>Sperm-Ovum Interactions</topic><topic>Spermatozoa - physiology</topic><topic>Vertebrates: reproduction</topic><topic>zona pellucida</topic><topic>Zona Pellucida - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marín-Briggiler, Clara I</creatorcontrib><creatorcontrib>Gonzalez-Echeverría, Fernanda</creatorcontrib><creatorcontrib>Buffone, Mariano</creatorcontrib><creatorcontrib>Calamera, Juan C</creatorcontrib><creatorcontrib>Tezón, Jorge G</creatorcontrib><creatorcontrib>Vazquez-Levin, Mónica H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marín-Briggiler, Clara I</au><au>Gonzalez-Echeverría, Fernanda</au><au>Buffone, Mariano</au><au>Calamera, Juan C</au><au>Tezón, Jorge G</au><au>Vazquez-Levin, Mónica H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium requirements for human sperm function in vitro</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>79</volume><issue>6</issue><spage>1396</spage><epage>1403</epage><pages>1396-1403</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To determine extracellular calcium (Ca
2+) requirements for the maintenance of human sperm function in vitro.
Prospective study.
Basic research laboratory.
Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET.
Spermatozoa were incubated for ≤18 hours in media containing different CaCl
2 concentrations (maximum, 2.5 mM [control]).
Protein tyrosine phosphorylation patterns, development of hyperactivated motility, induction of the acrosome reaction (AR) in response to human FF, and sperm interaction with homologous zona pellucida (ZP).
Cells maintained for 18 hours in medium containing ≥0.1 mM of Ca
2+ were able to undergo the AR when exposed to human FF in the presence of 2.5 mM of Ca
2+. Calcium concentrations of ≥0.22 mM were sufficient to reach protein tyrosine phosphorylation levels and hyperactivated motility values similar to those of controls. Higher Ca
2+ concentrations (≥0.58 mM) were required to produce maximum human FF–induced AR in previously capacitated cells and to obtain an adequate sperm–ZP binding.
Different steps of the fertilization process have distinctive Ca
2+ requirements. Whereas 0.22 mM of Ca
2+ is sufficient for the development of some capacitation-related events, human FF–induced AR and sperm–ZP interaction require 0.58 mM of this cation.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12798888</pmid><doi>10.1016/S0015-0282(03)00267-X</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acrosome - physiology Acrosome reaction Biological and medical sciences calcium Calcium - pharmacology capacitation Fundamental and applied biological sciences. Psychology human spermatozoa Humans In Vitro Techniques Male Mammalian male genital system Morphology. Physiology Prospective Studies Sperm Capacitation - physiology Sperm-Ovum Interactions Spermatozoa - physiology Vertebrates: reproduction zona pellucida Zona Pellucida - physiology |
| title | Calcium requirements for human sperm function in vitro |
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