The Anticodon Triplet is not Sufficient to Confer Methionine Acceptance to a Transfer RNA
Previous work suggested that the presence of the anticodon CAU alone was enough to confer methionine acceptance to a tRNA. Conversions of Escherichia coli non-methionine tRNAs to a methionine-accepting species were obtained by substitutions reconstructing the whole methionine anticodon loop together...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1992-11, Vol.89 (22), p.10768-10771 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Senger, Bruno Despons, Laurence Walter, Philippe Fasiolo, Franco |
| description | Previous work suggested that the presence of the anticodon CAU alone was enough to confer methionine acceptance to a tRNA. Conversions of Escherichia coli non-methionine tRNAs to a methionine-accepting species were obtained by substitutions reconstructing the whole methionine anticodon loop together with preservation (or introduction) of the acceptor stem base A73. We show here that the CAU triplet alone is unable to confer methionine acceptance when transplanted into a yeast aspartic tRNA. Both non-anticodon bases of the anticodon loop of yeast tRNAMetand A73 are required in addition to CAU for methionine acceptance. The importance of these non-anticodon bases in other CAU-containing tRNA frameworks was also established. These specific non-anticodon base interactions make a substantial thermodynamic contribution to the methionine acceptance of a transfer RNA. |
| doi_str_mv | 10.1073/pnas.89.22.10768 |
| format | Article |
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Conversions of Escherichia coli non-methionine tRNAs to a methionine-accepting species were obtained by substitutions reconstructing the whole methionine anticodon loop together with preservation (or introduction) of the acceptor stem base A73. We show here that the CAU triplet alone is unable to confer methionine acceptance when transplanted into a yeast aspartic tRNA. Both non-anticodon bases of the anticodon loop of yeast tRNAMetand A73 are required in addition to CAU for methionine acceptance. The importance of these non-anticodon bases in other CAU-containing tRNA frameworks was also established. These specific non-anticodon base interactions make a substantial thermodynamic contribution to the methionine acceptance of a transfer RNA.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.89.22.10768</identifier><identifier>PMID: 1438273</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Anticodon ; Anticodon - genetics ; Anticodon - metabolism ; Base Sequence ; Biochemistry ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Chemical bases ; Enzymes ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Hydrogen ; Kinetics ; Life Sciences ; Methionine - metabolism ; Models, Structural ; Molecular and cellular biology ; Molecular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic Acid Conformation ; Nucleic acids ; Nucleotides ; Replication ; Ribonucleic acid ; RNA ; RNA, Transfer, Met - genetics ; RNA, Transfer, Met - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Transcription, Genetic ; Transfer RNA ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1992-11, Vol.89 (22), p.10768-10771</ispartof><rights>Copyright 1992 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Nov 15, 1992</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-a4b93fcac4c62c7055685d2933d316faf79a366ae15db986a3adfb034c594d363</citedby><orcidid>0000-0002-5157-7307</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/89/22.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2361992$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2361992$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4496805$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1438273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02369845$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Senger, Bruno</creatorcontrib><creatorcontrib>Despons, Laurence</creatorcontrib><creatorcontrib>Walter, Philippe</creatorcontrib><creatorcontrib>Fasiolo, Franco</creatorcontrib><title>The Anticodon Triplet is not Sufficient to Confer Methionine Acceptance to a Transfer RNA</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Previous work suggested that the presence of the anticodon CAU alone was enough to confer methionine acceptance to a tRNA. Conversions of Escherichia coli non-methionine tRNAs to a methionine-accepting species were obtained by substitutions reconstructing the whole methionine anticodon loop together with preservation (or introduction) of the acceptor stem base A73. We show here that the CAU triplet alone is unable to confer methionine acceptance when transplanted into a yeast aspartic tRNA. Both non-anticodon bases of the anticodon loop of yeast tRNAMetand A73 are required in addition to CAU for methionine acceptance. The importance of these non-anticodon bases in other CAU-containing tRNA frameworks was also established. These specific non-anticodon base interactions make a substantial thermodynamic contribution to the methionine acceptance of a transfer RNA.</description><subject>Anticodon</subject><subject>Anticodon - genetics</subject><subject>Anticodon - metabolism</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Chemical bases</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Methionine - metabolism</subject><subject>Models, Structural</subject><subject>Molecular and cellular biology</subject><subject>Molecular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic acids</subject><subject>Nucleotides</subject><subject>Replication</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Transfer, Met - genetics</subject><subject>RNA, Transfer, Met - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Transcription, Genetic</subject><subject>Transfer RNA</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks-LEzEUx4Moa63ePSgOsogeWvN7EvBSyuoKVUHrwVNIM4lNmSbjJLPof2_G1rruQU8h-X6-eY_vewA8RHCOYE1edkGnuZBzjMc7F7fABEGJZpxKeBtMIMT1TFBM74J7Ke0ghJIJeAbOECUC12QCvqy3tlqE7E1sYqjWve9amyufqhBz9WlwzhtvQ65yrJYxONtX72ze-hh8KEZjbJd1MHbUdbHrkEbm4_vFfXDH6TbZB8dzCj6_vlgvL2erD2_eLhermWFC5JmmG0mc0YYajk0NGeOCNVgS0hDEnXa11IRzbRFrNlJwTXTjNpBQwyRtCCdT8Orwbzds9rYxpdlet6rr_V73P1TUXv2tBL9VX-OVYpBiUuwvDvbtDdPlYqXGN4gJl4KyK1TYZ8dSffw22JTV3idj21YHG4ekakKwqPH_QcQJpQyO4NMb4C4OfSh5KQwR5vUYxBTAA2T6mFJv3alPBNW4BmpcAyWkwlj9WoNieXw9lD-Gw9yLfn7UdTK6dWVuxqcTRqnkArKCPT9iY4Hf6rVCyg1tm-33XNAn_0YL8ehA7FKO_Qkp8SIpMfkJNEDbww</recordid><startdate>19921115</startdate><enddate>19921115</enddate><creator>Senger, Bruno</creator><creator>Despons, Laurence</creator><creator>Walter, Philippe</creator><creator>Fasiolo, Franco</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5157-7307</orcidid></search><sort><creationdate>19921115</creationdate><title>The Anticodon Triplet is not Sufficient to Confer Methionine Acceptance to a Transfer RNA</title><author>Senger, Bruno ; Despons, Laurence ; Walter, Philippe ; Fasiolo, Franco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-a4b93fcac4c62c7055685d2933d316faf79a366ae15db986a3adfb034c594d363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Anticodon</topic><topic>Anticodon - genetics</topic><topic>Anticodon - metabolism</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Chemical bases</topic><topic>Enzymes</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Methionine - metabolism</topic><topic>Models, Structural</topic><topic>Molecular and cellular biology</topic><topic>Molecular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic acids</topic><topic>Nucleotides</topic><topic>Replication</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Transfer, Met - genetics</topic><topic>RNA, Transfer, Met - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Transcription, Genetic</topic><topic>Transfer RNA</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Senger, Bruno</creatorcontrib><creatorcontrib>Despons, Laurence</creatorcontrib><creatorcontrib>Walter, Philippe</creatorcontrib><creatorcontrib>Fasiolo, Franco</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Senger, Bruno</au><au>Despons, Laurence</au><au>Walter, Philippe</au><au>Fasiolo, Franco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Anticodon Triplet is not Sufficient to Confer Methionine Acceptance to a Transfer RNA</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1992-11-15</date><risdate>1992</risdate><volume>89</volume><issue>22</issue><spage>10768</spage><epage>10771</epage><pages>10768-10771</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Previous work suggested that the presence of the anticodon CAU alone was enough to confer methionine acceptance to a tRNA. Conversions of Escherichia coli non-methionine tRNAs to a methionine-accepting species were obtained by substitutions reconstructing the whole methionine anticodon loop together with preservation (or introduction) of the acceptor stem base A73. We show here that the CAU triplet alone is unable to confer methionine acceptance when transplanted into a yeast aspartic tRNA. Both non-anticodon bases of the anticodon loop of yeast tRNAMetand A73 are required in addition to CAU for methionine acceptance. The importance of these non-anticodon bases in other CAU-containing tRNA frameworks was also established. These specific non-anticodon base interactions make a substantial thermodynamic contribution to the methionine acceptance of a transfer RNA.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1438273</pmid><doi>10.1073/pnas.89.22.10768</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-5157-7307</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Anticodon Anticodon - genetics Anticodon - metabolism Base Sequence Biochemistry Biochemistry, Molecular Biology Biological and medical sciences Chemical bases Enzymes Escherichia coli Fundamental and applied biological sciences. Psychology Hydrogen Kinetics Life Sciences Methionine - metabolism Models, Structural Molecular and cellular biology Molecular biology Molecular genetics Molecular Sequence Data Nucleic Acid Conformation Nucleic acids Nucleotides Replication Ribonucleic acid RNA RNA, Transfer, Met - genetics RNA, Transfer, Met - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Transcription, Genetic Transfer RNA Yeasts |
| title | The Anticodon Triplet is not Sufficient to Confer Methionine Acceptance to a Transfer RNA |
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