Purification of lipoxygenase and hydroperoxide dehydrase in flaxseeds: Interaction between these enzymatic activities
We have purified two enzymic activities from flaxseed acetone powder : a lipoxygenase and a hydroperoxide dehydrase. The lipoxygenase activity belongs to an iron-containing protein having a molecular weight of 130 kDa which, upon incubation with α-linolenic acid, forms 13-hydroperoxy-9(Z), 11 (E), 1...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-10, Vol.188 (2), p.858-864 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Rabinovitch-Chable, Hélène Cook-Moreau, Jeanne Breton, Jean-Christian Rigaud, Michel |
| description | We have purified two enzymic activities from flaxseed acetone powder : a lipoxygenase and a hydroperoxide dehydrase. The lipoxygenase activity belongs to an iron-containing protein having a molecular weight of 130 kDa which, upon incubation with α-linolenic acid, forms 13-hydroperoxy-9(Z), 11 (E), 15(Z)- octadecatrienoic acid. The hydroperoxide dehydrase (a 55 kDa protein) metabolizes this hydroperoxide to an allene oxide which in turn is spontaneously hydrolyzed to α-and γ-ketols. Relationships between these two enzymes were studied and results suggest an inhibition of the lipoxygenase by hydroperoxide dehydrase. |
| doi_str_mv | 10.1016/0006-291X(92)91135-D |
| format | Article |
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The lipoxygenase activity belongs to an iron-containing protein having a molecular weight of 130 kDa which, upon incubation with α-linolenic acid, forms 13-hydroperoxy-9(Z), 11 (E), 15(Z)- octadecatrienoic acid. The hydroperoxide dehydrase (a 55 kDa protein) metabolizes this hydroperoxide to an allene oxide which in turn is spontaneously hydrolyzed to α-and γ-ketols. 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Psychology ; GRAINE ; HIDROCARBUROS ; Hydro-Lyases - isolation & purification ; Hydro-Lyases - metabolism ; HYDROCARBURE ; INHIBICION ; INHIBITION ; Kinetics ; LIASAS ; Linolenic Acids - metabolism ; LINUM USITATISSIMUM ; LIPOXIGENASA ; LIPOXYGENASE ; Lipoxygenase - isolation & purification ; Lipoxygenase - metabolism ; LYASE ; METABOLITE ; METABOLITOS ; Oxidoreductases ; OXIDOS ; OXYDE ; PEROXIDOS ; PEROXYDE ; PURIFICACION ; PURIFICATION ; Seeds - enzymology ; SEMILLA ; VIA BIOQUIMICA DEL METABOLISMO ; VOIE BIOCHIMIQUE DU METABOLISME</subject><ispartof>Biochemical and biophysical research communications, 1992-10, Vol.188 (2), p.858-864</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-c92aa308fe93b70a0123f1091e8769b1dda6fa00a53bb9ad740cd6b95c60ce03</citedby><cites>FETCH-LOGICAL-c405t-c92aa308fe93b70a0123f1091e8769b1dda6fa00a53bb9ad740cd6b95c60ce03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(92)91135-D$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4359466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1359887$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rabinovitch-Chable, Hélène</creatorcontrib><creatorcontrib>Cook-Moreau, Jeanne</creatorcontrib><creatorcontrib>Breton, Jean-Christian</creatorcontrib><creatorcontrib>Rigaud, Michel</creatorcontrib><title>Purification of lipoxygenase and hydroperoxide dehydrase in flaxseeds: Interaction between these enzymatic activities</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have purified two enzymic activities from flaxseed acetone powder : a lipoxygenase and a hydroperoxide dehydrase. The lipoxygenase activity belongs to an iron-containing protein having a molecular weight of 130 kDa which, upon incubation with α-linolenic acid, forms 13-hydroperoxy-9(Z), 11 (E), 15(Z)- octadecatrienoic acid. The hydroperoxide dehydrase (a 55 kDa protein) metabolizes this hydroperoxide to an allene oxide which in turn is spontaneously hydrolyzed to α-and γ-ketols. Relationships between these two enzymes were studied and results suggest an inhibition of the lipoxygenase by hydroperoxide dehydrase.</description><subject>ACIDE LINOLENIQUE</subject><subject>ACIDO LINOLENICO</subject><subject>ACTIVIDAD CATALITICA</subject><subject>ACTIVITE CATALYTIQUE</subject><subject>alpha-Linolenic Acid</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Gel</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GRAINE</subject><subject>HIDROCARBUROS</subject><subject>Hydro-Lyases - isolation & purification</subject><subject>Hydro-Lyases - metabolism</subject><subject>HYDROCARBURE</subject><subject>INHIBICION</subject><subject>INHIBITION</subject><subject>Kinetics</subject><subject>LIASAS</subject><subject>Linolenic Acids - metabolism</subject><subject>LINUM USITATISSIMUM</subject><subject>LIPOXIGENASA</subject><subject>LIPOXYGENASE</subject><subject>Lipoxygenase - isolation & purification</subject><subject>Lipoxygenase - metabolism</subject><subject>LYASE</subject><subject>METABOLITE</subject><subject>METABOLITOS</subject><subject>Oxidoreductases</subject><subject>OXIDOS</subject><subject>OXYDE</subject><subject>PEROXIDOS</subject><subject>PEROXYDE</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>Seeds - enzymology</subject><subject>SEMILLA</subject><subject>VIA BIOQUIMICA DEL METABOLISMO</subject><subject>VOIE BIOCHIMIQUE DU METABOLISME</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFDEUhoModa3-AVGYCxG9GD2Zj8zGC0FaPwoFBSt4F84kJ21kNtkmM3XXX2-ms9Q7r0J4n_NyzsPYMw5vOHDxFgBEWUn-85WsXkvO67Y8vcdWHCSUFYfmPlvdIQ_Zo5R-AXDeCHnEjjIs1-tuxaZvU3TWaRxd8EWwxeC2Ybe_JI-JCvSmuNqbGLYUw84ZKgzN_zlzvrAD7hKRSe-KMz9SRH3b0tP4m8gX4xVljvyf_SbX62KOb9zoKD1mDywOiZ4c3mN28enjxcmX8vzr57OTD-elbqAdSy0rxBrWlmTdd4DAq9rm-zitOyF7bgwKiwDY1n0v0XQNaCN62WoBmqA-Zi-X2m0M1xOlUW1c0jQM6ClMSXV1XXWVqDLYLKCOIaVIVm2j22DcKw5qlq1mk2o2qWSlbmWr0zz2_NA_9Rsy_4YWuzl_ccgxaRxsRK9dusOajDVCZOzpglkMCi9jRn58lzWXdTuv9n4JKXu6cRRV0o68JuMi6VGZ4P6_5F9556aq</recordid><startdate>19921030</startdate><enddate>19921030</enddate><creator>Rabinovitch-Chable, Hélène</creator><creator>Cook-Moreau, Jeanne</creator><creator>Breton, Jean-Christian</creator><creator>Rigaud, Michel</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921030</creationdate><title>Purification of lipoxygenase and hydroperoxide dehydrase in flaxseeds: Interaction between these enzymatic activities</title><author>Rabinovitch-Chable, Hélène ; Cook-Moreau, Jeanne ; Breton, Jean-Christian ; Rigaud, Michel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-c92aa308fe93b70a0123f1091e8769b1dda6fa00a53bb9ad740cd6b95c60ce03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACIDE LINOLENIQUE</topic><topic>ACIDO LINOLENICO</topic><topic>ACTIVIDAD CATALITICA</topic><topic>ACTIVITE CATALYTIQUE</topic><topic>alpha-Linolenic Acid</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Gel</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GRAINE</topic><topic>HIDROCARBUROS</topic><topic>Hydro-Lyases - isolation & purification</topic><topic>Hydro-Lyases - metabolism</topic><topic>HYDROCARBURE</topic><topic>INHIBICION</topic><topic>INHIBITION</topic><topic>Kinetics</topic><topic>LIASAS</topic><topic>Linolenic Acids - metabolism</topic><topic>LINUM USITATISSIMUM</topic><topic>LIPOXIGENASA</topic><topic>LIPOXYGENASE</topic><topic>Lipoxygenase - isolation & purification</topic><topic>Lipoxygenase - metabolism</topic><topic>LYASE</topic><topic>METABOLITE</topic><topic>METABOLITOS</topic><topic>Oxidoreductases</topic><topic>OXIDOS</topic><topic>OXYDE</topic><topic>PEROXIDOS</topic><topic>PEROXYDE</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>Seeds - enzymology</topic><topic>SEMILLA</topic><topic>VIA BIOQUIMICA DEL METABOLISMO</topic><topic>VOIE BIOCHIMIQUE DU METABOLISME</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rabinovitch-Chable, Hélène</creatorcontrib><creatorcontrib>Cook-Moreau, Jeanne</creatorcontrib><creatorcontrib>Breton, Jean-Christian</creatorcontrib><creatorcontrib>Rigaud, Michel</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rabinovitch-Chable, Hélène</au><au>Cook-Moreau, Jeanne</au><au>Breton, Jean-Christian</au><au>Rigaud, Michel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of lipoxygenase and hydroperoxide dehydrase in flaxseeds: Interaction between these enzymatic activities</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-10-30</date><risdate>1992</risdate><volume>188</volume><issue>2</issue><spage>858</spage><epage>864</epage><pages>858-864</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>We have purified two enzymic activities from flaxseed acetone powder : a lipoxygenase and a hydroperoxide dehydrase. The lipoxygenase activity belongs to an iron-containing protein having a molecular weight of 130 kDa which, upon incubation with α-linolenic acid, forms 13-hydroperoxy-9(Z), 11 (E), 15(Z)- octadecatrienoic acid. The hydroperoxide dehydrase (a 55 kDa protein) metabolizes this hydroperoxide to an allene oxide which in turn is spontaneously hydrolyzed to α-and γ-ketols. Relationships between these two enzymes were studied and results suggest an inhibition of the lipoxygenase by hydroperoxide dehydrase.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1359887</pmid><doi>10.1016/0006-291X(92)91135-D</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1992-10, Vol.188 (2), p.858-864 |
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| subjects | ACIDE LINOLENIQUE ACIDO LINOLENICO ACTIVIDAD CATALITICA ACTIVITE CATALYTIQUE alpha-Linolenic Acid Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology GRAINE HIDROCARBUROS Hydro-Lyases - isolation & purification Hydro-Lyases - metabolism HYDROCARBURE INHIBICION INHIBITION Kinetics LIASAS Linolenic Acids - metabolism LINUM USITATISSIMUM LIPOXIGENASA LIPOXYGENASE Lipoxygenase - isolation & purification Lipoxygenase - metabolism LYASE METABOLITE METABOLITOS Oxidoreductases OXIDOS OXYDE PEROXIDOS PEROXYDE PURIFICACION PURIFICATION Seeds - enzymology SEMILLA VIA BIOQUIMICA DEL METABOLISMO VOIE BIOCHIMIQUE DU METABOLISME |
| title | Purification of lipoxygenase and hydroperoxide dehydrase in flaxseeds: Interaction between these enzymatic activities |
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