The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes
Type 1 diabetes mellitus (T1DM) is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesi...
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| Veröffentlicht in: | Folia histochemica et cytobiologica 2010-01, Vol.48 (1), p.93-100 |
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| creator | Łuczyński, Włodzimierz Stasiak-Barmuta, Anna Juchniewicz, Agnieszka Wawrusiewicz-Kurylonek, Natalia Iłendo, Elzbieta Kos, Justyna Kretowski, Adam Górska, Maria Chyczewski, Lech Bossowski, Artur |
| description | Type 1 diabetes mellitus (T1DM) is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group) were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha), activatory molecules (OX40, 4-1BB) and elements of cytotoxicity (granzyme B, perforin 1) were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted. |
| doi_str_mv | 10.2478/v10042-008-0113-5 |
| format | Article |
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T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group) were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha), activatory molecules (OX40, 4-1BB) and elements of cytotoxicity (granzyme B, perforin 1) were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted.</description><identifier>ISSN: 0239-8508</identifier><identifier>EISSN: 1897-5631</identifier><identifier>DOI: 10.2478/v10042-008-0113-5</identifier><identifier>PMID: 20529823</identifier><language>eng</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Autoimmune diseases ; Beta cells ; Case-Control Studies ; CD134 antigen ; CD137 antigen ; CD25 antigen ; CD3 antigen ; CD4 antigen ; Child ; Children ; Cytokines ; Cytokines - genetics ; Cytokines - metabolism ; Cytotoxicity ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (insulin dependent) ; Diabetes Mellitus, Type 1 - genetics ; Diabetes Mellitus, Type 1 - immunology ; Flow Cytometry ; Foxp3 protein ; Gene expression ; Gene Expression Regulation ; Granzyme B ; Growth factors ; Health ; Humans ; Immunological tolerance ; Immunoregulation ; Inflammation ; Inflammation Mediators - metabolism ; Insulin ; Interferon ; Interleukin 10 ; Interleukin 2 ; Interleukin 4 ; Interleukin-2 Receptor alpha Subunit - metabolism ; Interleukin-7 Receptor alpha Subunit - metabolism ; Lymphocyte Subsets - metabolism ; Lymphocytes ; Lymphocytes T ; Perforin ; Peripheral blood ; Receptors, OX40 - genetics ; Receptors, OX40 - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Subpopulations ; T-Lymphocytes, Regulatory - metabolism ; Toxicity ; Tumor Necrosis Factor Receptor Superfamily, Member 9 - genetics ; Tumor Necrosis Factor Receptor Superfamily, Member 9 - metabolism</subject><ispartof>Folia histochemica et cytobiologica, 2010-01, Vol.48 (1), p.93-100</ispartof><rights>2010. 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright Folia Histochemica et Cytobiologica 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-39569874d4b472bc8cbef1e27bffaf1640e0549e361e7c4c64b356d3415e84a43</citedby><cites>FETCH-LOGICAL-c355t-39569874d4b472bc8cbef1e27bffaf1640e0549e361e7c4c64b356d3415e84a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20529823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Łuczyński, Włodzimierz</creatorcontrib><creatorcontrib>Stasiak-Barmuta, Anna</creatorcontrib><creatorcontrib>Juchniewicz, Agnieszka</creatorcontrib><creatorcontrib>Wawrusiewicz-Kurylonek, Natalia</creatorcontrib><creatorcontrib>Iłendo, Elzbieta</creatorcontrib><creatorcontrib>Kos, Justyna</creatorcontrib><creatorcontrib>Kretowski, Adam</creatorcontrib><creatorcontrib>Górska, Maria</creatorcontrib><creatorcontrib>Chyczewski, Lech</creatorcontrib><creatorcontrib>Bossowski, Artur</creatorcontrib><title>The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes</title><title>Folia histochemica et cytobiologica</title><addtitle>Folia Histochem Cytobiol</addtitle><description>Type 1 diabetes mellitus (T1DM) is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group) were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha), activatory molecules (OX40, 4-1BB) and elements of cytotoxicity (granzyme B, perforin 1) were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted.</description><subject>Autoimmune diseases</subject><subject>Beta cells</subject><subject>Case-Control Studies</subject><subject>CD134 antigen</subject><subject>CD137 antigen</subject><subject>CD25 antigen</subject><subject>CD3 antigen</subject><subject>CD4 antigen</subject><subject>Child</subject><subject>Children</subject><subject>Cytokines</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Cytotoxicity</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (insulin dependent)</subject><subject>Diabetes Mellitus, Type 1 - genetics</subject><subject>Diabetes Mellitus, Type 1 - immunology</subject><subject>Flow Cytometry</subject><subject>Foxp3 protein</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Granzyme B</subject><subject>Growth factors</subject><subject>Health</subject><subject>Humans</subject><subject>Immunological tolerance</subject><subject>Immunoregulation</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Insulin</subject><subject>Interferon</subject><subject>Interleukin 10</subject><subject>Interleukin 2</subject><subject>Interleukin 4</subject><subject>Interleukin-2 Receptor alpha Subunit - metabolism</subject><subject>Interleukin-7 Receptor alpha Subunit - metabolism</subject><subject>Lymphocyte Subsets - metabolism</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Perforin</subject><subject>Peripheral blood</subject><subject>Receptors, OX40 - genetics</subject><subject>Receptors, OX40 - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Subpopulations</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>Toxicity</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 9 - genetics</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 9 - metabolism</subject><issn>0239-8508</issn><issn>1897-5631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kc1u1DAUha0KRIeWB2CDLFiwcrn-d5ZVVaBS1UrVsLac5Kbjkp_BTlrm7Uk6QxdILK4sXX8-OtZHyHsOZ0JZ9-WRAyjBABwDziXTR2TFXWGZNpK_IisQsmBOgzsmb3N-ANAGFH9DjgVoUTghVyStN0i7u5tzir-3CXOOQ0-Hhm7TwGjo63nGyGLftKHrwjikHa124_Az9php7OmaJryf2sMNtu3zttrEtk7Y06c4bui42yLltI6hxBHzKXndhDbju8N5Qn58vVxffGfXt9-uLs6vWSW1HpkstCmcVbUqlRVl5aoSG47Clk0TGm4UIGhVoDQcbaUqo0qpTS0V1-hUUPKEfN7nzn_5NWEefRfzUjH0OEzZWymFLrQwM_nxH_JhmFI_l_NaWMvBcT1Dn_4HCVMIB0bxJYrvqSoNOSds_DbFLqSd5-AXaX4vzc_S_CLNL8kfDslT2WH98uKvJfkHfu-Q-A</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Łuczyński, Włodzimierz</creator><creator>Stasiak-Barmuta, Anna</creator><creator>Juchniewicz, Agnieszka</creator><creator>Wawrusiewicz-Kurylonek, Natalia</creator><creator>Iłendo, Elzbieta</creator><creator>Kos, Justyna</creator><creator>Kretowski, Adam</creator><creator>Górska, Maria</creator><creator>Chyczewski, Lech</creator><creator>Bossowski, Artur</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20100101</creationdate><title>The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes</title><author>Łuczyński, Włodzimierz ; Stasiak-Barmuta, Anna ; Juchniewicz, Agnieszka ; Wawrusiewicz-Kurylonek, Natalia ; Iłendo, Elzbieta ; Kos, Justyna ; Kretowski, Adam ; Górska, Maria ; Chyczewski, Lech ; Bossowski, Artur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-39569874d4b472bc8cbef1e27bffaf1640e0549e361e7c4c64b356d3415e84a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Autoimmune diseases</topic><topic>Beta cells</topic><topic>Case-Control Studies</topic><topic>CD134 antigen</topic><topic>CD137 antigen</topic><topic>CD25 antigen</topic><topic>CD3 antigen</topic><topic>CD4 antigen</topic><topic>Child</topic><topic>Children</topic><topic>Cytokines</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Cytotoxicity</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes mellitus (insulin dependent)</topic><topic>Diabetes Mellitus, Type 1 - genetics</topic><topic>Diabetes Mellitus, Type 1 - immunology</topic><topic>Flow Cytometry</topic><topic>Foxp3 protein</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Granzyme B</topic><topic>Growth factors</topic><topic>Health</topic><topic>Humans</topic><topic>Immunological tolerance</topic><topic>Immunoregulation</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Insulin</topic><topic>Interferon</topic><topic>Interleukin 10</topic><topic>Interleukin 2</topic><topic>Interleukin 4</topic><topic>Interleukin-2 Receptor alpha Subunit - metabolism</topic><topic>Interleukin-7 Receptor alpha Subunit - metabolism</topic><topic>Lymphocyte Subsets - metabolism</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Perforin</topic><topic>Peripheral blood</topic><topic>Receptors, OX40 - 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Academic</collection><jtitle>Folia histochemica et cytobiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Łuczyński, Włodzimierz</au><au>Stasiak-Barmuta, Anna</au><au>Juchniewicz, Agnieszka</au><au>Wawrusiewicz-Kurylonek, Natalia</au><au>Iłendo, Elzbieta</au><au>Kos, Justyna</au><au>Kretowski, Adam</au><au>Górska, Maria</au><au>Chyczewski, Lech</au><au>Bossowski, Artur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes</atitle><jtitle>Folia histochemica et cytobiologica</jtitle><addtitle>Folia Histochem Cytobiol</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>48</volume><issue>1</issue><spage>93</spage><epage>100</epage><pages>93-100</pages><issn>0239-8508</issn><eissn>1897-5631</eissn><abstract>Type 1 diabetes mellitus (T1DM) is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group) were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha), activatory molecules (OX40, 4-1BB) and elements of cytotoxicity (granzyme B, perforin 1) were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>20529823</pmid><doi>10.2478/v10042-008-0113-5</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Folia histochemica et cytobiologica, 2010-01, Vol.48 (1), p.93-100 |
| issn | 0239-8508 1897-5631 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Autoimmune diseases Beta cells Case-Control Studies CD134 antigen CD137 antigen CD25 antigen CD3 antigen CD4 antigen Child Children Cytokines Cytokines - genetics Cytokines - metabolism Cytotoxicity Diabetes Diabetes mellitus Diabetes mellitus (insulin dependent) Diabetes Mellitus, Type 1 - genetics Diabetes Mellitus, Type 1 - immunology Flow Cytometry Foxp3 protein Gene expression Gene Expression Regulation Granzyme B Growth factors Health Humans Immunological tolerance Immunoregulation Inflammation Inflammation Mediators - metabolism Insulin Interferon Interleukin 10 Interleukin 2 Interleukin 4 Interleukin-2 Receptor alpha Subunit - metabolism Interleukin-7 Receptor alpha Subunit - metabolism Lymphocyte Subsets - metabolism Lymphocytes Lymphocytes T Perforin Peripheral blood Receptors, OX40 - genetics Receptors, OX40 - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Subpopulations T-Lymphocytes, Regulatory - metabolism Toxicity Tumor Necrosis Factor Receptor Superfamily, Member 9 - genetics Tumor Necrosis Factor Receptor Superfamily, Member 9 - metabolism |
| title | The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T19%3A12%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20mRNA%20expression%20of%20pro-%20and%20anti-inflammatory%20cytokines%20in%20T%20regulatory%20cells%20in%20children%20with%20type%201%20diabetes&rft.jtitle=Folia%20histochemica%20et%20cytobiologica&rft.au=%C5%81uczy%C5%84ski,%20W%C5%82odzimierz&rft.date=2010-01-01&rft.volume=48&rft.issue=1&rft.spage=93&rft.epage=100&rft.pages=93-100&rft.issn=0239-8508&rft.eissn=1897-5631&rft_id=info:doi/10.2478/v10042-008-0113-5&rft_dat=%3Cproquest_cross%3E733259526%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2692806416&rft_id=info:pmid/20529823&rfr_iscdi=true |