Expression and purification of recombinant NFI proteins for functional analysis

Nuclear factor I (NFI) is a transcription factor playing wide role in signal transduction pathways and developmental processes in higher eukaryotes. In order to produce recombinant NFI proteins for functional and structural studies, full length cDNAs of individual isoforms were subcloned into pETM30...

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Veröffentlicht in:	General physiology and biophysics 2009-12, Vol.28 (4), p.331-339
Hauptverfasser:	Kollárovic, Gabriel, Majera, Dusana, Luciaková, Katarína, Baráth, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - immunology Blotting, Western Cell Extracts Cell Nucleus - metabolism DNA - metabolism Electrophoresis, Polyacrylamide Gel Gene Expression Immunoassay Immunoprecipitation NFI Transcription Factors - genetics NFI Transcription Factors - immunology NFI Transcription Factors - isolation & purification NFI Transcription Factors - metabolism Protein Isoforms - genetics Protein Isoforms - immunology Protein Isoforms - isolation & purification Protein Isoforms - metabolism Rats Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism
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