Stop-transfer regions do not halt translocation of proteins into chloroplasts
Protein targeting in eukaryotic cells is determined by several topogenic signals. Among these are stop-transfer regions, which halt translocation of proteins across the endoplasmic reticulum membrane. Two different stop-transfer regions were incorporated into precursors for a chloroplast protein, th...
Gespeichert in:
| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1987-11, Vol.238 (4830), p.1112-1114 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1114 |
|---|---|
| container_issue | 4830 |
| container_start_page | 1112 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 238 |
| creator | Lubben, T.H Bansberg, J Keegstra, K |
| description | Protein targeting in eukaryotic cells is determined by several topogenic signals. Among these are stop-transfer regions, which halt translocation of proteins across the endoplasmic reticulum membrane. Two different stop-transfer regions were incorporated into precursors for a chloroplast protein, the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Both chimeric proteins were imported into chloroplasts and did not accumulate in the envelope membranes. Thus, the stop-transfer signals did not function during chloroplast protein import. These observations support the hypothesis that the mechanism for translocation of proteins across the chloroplast envelope is significantly different from that for translocation across the endoplasmic reticulum membrane. |
| doi_str_mv | 10.1126/science.238.4830.1112 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_733243416</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A6152521</galeid><jstor_id>1700850</jstor_id><sourcerecordid>A6152521</sourcerecordid><originalsourceid>FETCH-LOGICAL-c734t-61703d0ce745e4070a182829e974dc3a8cff01805fe094bf710b0682d789c3d03</originalsourceid><addsrcrecordid>eNqN0l1v0zAUBuAIgVgZ_ANAEULsYqT4I7Gdy1FBmVToRRm3luscZ6ncuLNdCf49Dq1YQRVUuYjk85xjx3mz7AVGY4wJexd0B72GMaFiXAo6rGLyIBthVFdFTRB9mI0QoqwQiFdn2ZMQVgilWk0fZ2eYC1pTxkfZ50V0myJ61QcDPvfQdq4PeePy3sX8VtmY_ypap1VMpdyZfONdhC6pro8u17fWebexKsTwNHtklA3wbP8-z24-fvg6-VTM5tPrydWs0JyWsWCYI9ogDbysoEQcKSyIIDXUvGw0VUIbg7BAlQFUl0vDMVoiJkjDRa1TIz3PLnZz01HuthCiXHdBg7WqB7cNklNKSlpiluSbf0rKSEWGW_kfxKWoGGYDfPUXXLmt79PnSoJpGsYETuhyh1plQXa9cekWdQs9eGVdD6ZLy1cMV2n3Qb89otPTwLrTR_jFHzyJCN9jq7YhyOvFl1Pl_Nup8v30RCmms0N5eUxqZy20IFMiJvNDXe209i4ED0ZufLdW_ofESA6Rl_vIyxR5OUReDpFPfS_3_2O7XENz37XPeAKv90AFraxJcdZd-O14RXnJRGLPd2wVovMHYxASFbovG-Wkan2acLMQIh2eCSHoT6fSF3M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213529681</pqid></control><display><type>article</type><title>Stop-transfer regions do not halt translocation of proteins into chloroplasts</title><source>American Association for the Advancement of Science</source><source>Jstor Complete Legacy</source><creator>Lubben, T.H ; Bansberg, J ; Keegstra, K</creator><creatorcontrib>Lubben, T.H ; Bansberg, J ; Keegstra, K</creatorcontrib><description>Protein targeting in eukaryotic cells is determined by several topogenic signals. Among these are stop-transfer regions, which halt translocation of proteins across the endoplasmic reticulum membrane. Two different stop-transfer regions were incorporated into precursors for a chloroplast protein, the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Both chimeric proteins were imported into chloroplasts and did not accumulate in the envelope membranes. Thus, the stop-transfer signals did not function during chloroplast protein import. These observations support the hypothesis that the mechanism for translocation of proteins across the chloroplast envelope is significantly different from that for translocation across the endoplasmic reticulum membrane.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.238.4830.1112</identifier><identifier>PMID: 17839367</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: The American Association for the Advancement of Science</publisher><subject>Biological and medical sciences ; Biological transport ; Biology ; CELL MEMBRANES ; Cell physiology ; Centrifugation ; Chloroplasts ; endoplasmic reticulum ; FISIOLOGIA VEGETAL ; Fundamental and applied biological sciences. Psychology ; Imports ; Medical research ; MEMBRANAS CELULARES ; Membrane and intracellular transports ; MEMBRANE CELLULAIRE ; METABOLISME DES PROTEINES ; METABOLISMO DE PROTEINAS ; Molecular and cellular biology ; Molecules ; P branes ; PHYSIOLOGIE VEGETALE ; PLANT PHYSIOLOGY ; Plasmids ; PLASTE ; PLASTIDIOS ; PLASTIDS ; PROTEIN METABOLISM ; Protein precursors ; Protein research ; Protein transport ; Proteins ; String theory ; Thylakoids ; TRANSLOCACION ; TRANSLOCATION</subject><ispartof>Science (American Association for the Advancement of Science), 1987-11, Vol.238 (4830), p.1112-1114</ispartof><rights>Copyright 1987 The American Association for the Advancement of Science</rights><rights>1988 INIST-CNRS</rights><rights>COPYRIGHT 1987 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1987 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Nov 20, 1987</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c734t-61703d0ce745e4070a182829e974dc3a8cff01805fe094bf710b0682d789c3d03</citedby><cites>FETCH-LOGICAL-c734t-61703d0ce745e4070a182829e974dc3a8cff01805fe094bf710b0682d789c3d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1700850$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1700850$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,778,782,801,2873,2874,27913,27914,58006,58239</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7537468$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17839367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lubben, T.H</creatorcontrib><creatorcontrib>Bansberg, J</creatorcontrib><creatorcontrib>Keegstra, K</creatorcontrib><title>Stop-transfer regions do not halt translocation of proteins into chloroplasts</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Protein targeting in eukaryotic cells is determined by several topogenic signals. Among these are stop-transfer regions, which halt translocation of proteins across the endoplasmic reticulum membrane. Two different stop-transfer regions were incorporated into precursors for a chloroplast protein, the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Both chimeric proteins were imported into chloroplasts and did not accumulate in the envelope membranes. Thus, the stop-transfer signals did not function during chloroplast protein import. These observations support the hypothesis that the mechanism for translocation of proteins across the chloroplast envelope is significantly different from that for translocation across the endoplasmic reticulum membrane.</description><subject>Biological and medical sciences</subject><subject>Biological transport</subject><subject>Biology</subject><subject>CELL MEMBRANES</subject><subject>Cell physiology</subject><subject>Centrifugation</subject><subject>Chloroplasts</subject><subject>endoplasmic reticulum</subject><subject>FISIOLOGIA VEGETAL</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imports</subject><subject>Medical research</subject><subject>MEMBRANAS CELULARES</subject><subject>Membrane and intracellular transports</subject><subject>MEMBRANE CELLULAIRE</subject><subject>METABOLISME DES PROTEINES</subject><subject>METABOLISMO DE PROTEINAS</subject><subject>Molecular and cellular biology</subject><subject>Molecules</subject><subject>P branes</subject><subject>PHYSIOLOGIE VEGETALE</subject><subject>PLANT PHYSIOLOGY</subject><subject>Plasmids</subject><subject>PLASTE</subject><subject>PLASTIDIOS</subject><subject>PLASTIDS</subject><subject>PROTEIN METABOLISM</subject><subject>Protein precursors</subject><subject>Protein research</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>String theory</subject><subject>Thylakoids</subject><subject>TRANSLOCACION</subject><subject>TRANSLOCATION</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqN0l1v0zAUBuAIgVgZ_ANAEULsYqT4I7Gdy1FBmVToRRm3luscZ6ncuLNdCf49Dq1YQRVUuYjk85xjx3mz7AVGY4wJexd0B72GMaFiXAo6rGLyIBthVFdFTRB9mI0QoqwQiFdn2ZMQVgilWk0fZ2eYC1pTxkfZ50V0myJ61QcDPvfQdq4PeePy3sX8VtmY_ypap1VMpdyZfONdhC6pro8u17fWebexKsTwNHtklA3wbP8-z24-fvg6-VTM5tPrydWs0JyWsWCYI9ogDbysoEQcKSyIIDXUvGw0VUIbg7BAlQFUl0vDMVoiJkjDRa1TIz3PLnZz01HuthCiXHdBg7WqB7cNklNKSlpiluSbf0rKSEWGW_kfxKWoGGYDfPUXXLmt79PnSoJpGsYETuhyh1plQXa9cekWdQs9eGVdD6ZLy1cMV2n3Qb89otPTwLrTR_jFHzyJCN9jq7YhyOvFl1Pl_Nup8v30RCmms0N5eUxqZy20IFMiJvNDXe209i4ED0ZufLdW_ofESA6Rl_vIyxR5OUReDpFPfS_3_2O7XENz37XPeAKv90AFraxJcdZd-O14RXnJRGLPd2wVovMHYxASFbovG-Wkan2acLMQIh2eCSHoT6fSF3M</recordid><startdate>19871120</startdate><enddate>19871120</enddate><creator>Lubben, T.H</creator><creator>Bansberg, J</creator><creator>Keegstra, K</creator><general>The American Association for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>M7Z</scope><scope>7X8</scope></search><sort><creationdate>19871120</creationdate><title>Stop-transfer regions do not halt translocation of proteins into chloroplasts</title><author>Lubben, T.H ; Bansberg, J ; Keegstra, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c734t-61703d0ce745e4070a182829e974dc3a8cff01805fe094bf710b0682d789c3d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>Biological transport</topic><topic>Biology</topic><topic>CELL MEMBRANES</topic><topic>Cell physiology</topic><topic>Centrifugation</topic><topic>Chloroplasts</topic><topic>endoplasmic reticulum</topic><topic>FISIOLOGIA VEGETAL</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Imports</topic><topic>Medical research</topic><topic>MEMBRANAS CELULARES</topic><topic>Membrane and intracellular transports</topic><topic>MEMBRANE CELLULAIRE</topic><topic>METABOLISME DES PROTEINES</topic><topic>METABOLISMO DE PROTEINAS</topic><topic>Molecular and cellular biology</topic><topic>Molecules</topic><topic>P branes</topic><topic>PHYSIOLOGIE VEGETALE</topic><topic>PLANT PHYSIOLOGY</topic><topic>Plasmids</topic><topic>PLASTE</topic><topic>PLASTIDIOS</topic><topic>PLASTIDS</topic><topic>PROTEIN METABOLISM</topic><topic>Protein precursors</topic><topic>Protein research</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>String theory</topic><topic>Thylakoids</topic><topic>TRANSLOCACION</topic><topic>TRANSLOCATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lubben, T.H</creatorcontrib><creatorcontrib>Bansberg, J</creatorcontrib><creatorcontrib>Keegstra, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>Gale In Context: Biography</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Canada</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lubben, T.H</au><au>Bansberg, J</au><au>Keegstra, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stop-transfer regions do not halt translocation of proteins into chloroplasts</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1987-11-20</date><risdate>1987</risdate><volume>238</volume><issue>4830</issue><spage>1112</spage><epage>1114</epage><pages>1112-1114</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Protein targeting in eukaryotic cells is determined by several topogenic signals. Among these are stop-transfer regions, which halt translocation of proteins across the endoplasmic reticulum membrane. Two different stop-transfer regions were incorporated into precursors for a chloroplast protein, the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Both chimeric proteins were imported into chloroplasts and did not accumulate in the envelope membranes. Thus, the stop-transfer signals did not function during chloroplast protein import. These observations support the hypothesis that the mechanism for translocation of proteins across the chloroplast envelope is significantly different from that for translocation across the endoplasmic reticulum membrane.</abstract><cop>Washington, DC</cop><pub>The American Association for the Advancement of Science</pub><pmid>17839367</pmid><doi>10.1126/science.238.4830.1112</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1987-11, Vol.238 (4830), p.1112-1114 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733243416 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy |
| subjects | Biological and medical sciences Biological transport Biology CELL MEMBRANES Cell physiology Centrifugation Chloroplasts endoplasmic reticulum FISIOLOGIA VEGETAL Fundamental and applied biological sciences. Psychology Imports Medical research MEMBRANAS CELULARES Membrane and intracellular transports MEMBRANE CELLULAIRE METABOLISME DES PROTEINES METABOLISMO DE PROTEINAS Molecular and cellular biology Molecules P branes PHYSIOLOGIE VEGETALE PLANT PHYSIOLOGY Plasmids PLASTE PLASTIDIOS PLASTIDS PROTEIN METABOLISM Protein precursors Protein research Protein transport Proteins String theory Thylakoids TRANSLOCACION TRANSLOCATION |
| title | Stop-transfer regions do not halt translocation of proteins into chloroplasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T09%3A42%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stop-transfer%20regions%20do%20not%20halt%20translocation%20of%20proteins%20into%20chloroplasts&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Lubben,%20T.H&rft.date=1987-11-20&rft.volume=238&rft.issue=4830&rft.spage=1112&rft.epage=1114&rft.pages=1112-1114&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.238.4830.1112&rft_dat=%3Cgale_proqu%3EA6152521%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213529681&rft_id=info:pmid/17839367&rft_galeid=A6152521&rft_jstor_id=1700850&rfr_iscdi=true |