QDs-DNA nanosensor for the detection of hepatitis B virus DNA and the single-base mutants
We report here a quantum dots-DNA (QDs-DNA) nanosensor based on fluorescence resonance energy transfer (FRET) for the detection of the target DNA and single mismatch in hepatitis B virus (HBV) gene. The proposed one-pot DNA detection method is simple, rapid and efficient due to the elimination of th...
Gespeichert in:
Veröffentlicht in: | Biosensors & bioelectronics 2010-04, Vol.25 (8), p.1934-1940 |
---|---|
Hauptverfasser: | , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1940 |
---|---|
container_issue | 8 |
container_start_page | 1934 |
container_title | Biosensors & bioelectronics |
container_volume | 25 |
creator | Wang, Xiang Lou, Xinhui Wang, Yi Guo, Qingchuan Fang, Zheng Zhong, Xinhua Mao, Hongju Jin, Qinghui Wu, Lei Zhao, Hui Zhao, Jianlong |
description | We report here a quantum dots-DNA (QDs-DNA) nanosensor based on fluorescence resonance energy transfer (FRET) for the detection of the target DNA and single mismatch in hepatitis B virus (HBV) gene. The proposed one-pot DNA detection method is simple, rapid and efficient due to the elimination of the washing and separation steps. In this study, the water-soluble CdSe/ZnS QDs were prepared by replacing the trioctylphosphine oxide (TOPO) on the surface of QDs with 3-mercaptopropionic acid (MPA). Subsequently, oligonucleotides were attached to the QDs surface to form functional QDs-DNA conjugates. Along with the addition of DNA targets and Cy5-modified signal DNAs into the QDs-DNA conjugates, sandwiched hybrids were formed. The resulting assembly brings the Cy5 fluorophore, the acceptor, and the QDs, the donor, into proximity, leading to fluorescence emission from the acceptor by means of FRET on illumination of the donor. In order to efficiently detect single-base mutants in HBV gene, oligonucleotide ligation assay was employed. If there existed a single-base mismatch, which could be recognized by the ligase, the detection probe was not ligated and no Cy5 emission was produced due to the lack of FRET. The feasibility of the proposed method was also demonstrated in the detection of synthetic 30-mer oliginucleotide targets derived from the HBV with a sensitivity of 4.0
nM by using a multilabel counter. The method enables a simple and efficient detection that could be potentially used for high throughput and multiplex detections of target DNA and the mutants. |
doi_str_mv | 10.1016/j.bios.2010.01.007 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733231064</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0956566310000199</els_id><sourcerecordid>733231064</sourcerecordid><originalsourceid>FETCH-LOGICAL-c451t-af37c6bc0478ed7d84c791b8ff5d6384a387a28dbdf7ebc15890e8c4092209163</originalsourceid><addsrcrecordid>eNp9kU2LFDEQhoMo7uzqH_AguYinHvPR3UmDl3V3_YBFEfTgKaSTipuhJz2m0gv-e9POqDcPRUHxvEXxFCHPONtyxvtXu-0YZ9wKVgeMbxlTD8iGayWbVsjuIdmwoeubru_lGTlH3LFK8IE9Jmc1InU76A359vkam-uPlzTZNCMknDMNtcodUA8FXIlzonOgd3CwJZaI9A29j3lBuqZs8r9RjOn7BM1oEeh-KTYVfEIeBTshPD31C_L17c2Xq_fN7ad3H64ubxvXdrw0Nkjl-tGxVmnwyuvWqYGPOoTO9_VIK7WyQvvRBwWj450eGGjXskEINvBeXpCXx72HPP9YAIvZR3QwTTbBvKBRUgrJWd9WUhxJl2fEDMEcctzb_NNwZlajZmdWo2Y1ahg31VcNPT-tX8Y9-L-RPwor8OIEWHR2CtkmF_EfJ-oDhFy510cOqoz7CNmgi5Ac-JirZuPn-L87fgHUSJM3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733231064</pqid></control><display><type>article</type><title>QDs-DNA nanosensor for the detection of hepatitis B virus DNA and the single-base mutants</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Wang, Xiang ; Lou, Xinhui ; Wang, Yi ; Guo, Qingchuan ; Fang, Zheng ; Zhong, Xinhua ; Mao, Hongju ; Jin, Qinghui ; Wu, Lei ; Zhao, Hui ; Zhao, Jianlong</creator><creatorcontrib>Wang, Xiang ; Lou, Xinhui ; Wang, Yi ; Guo, Qingchuan ; Fang, Zheng ; Zhong, Xinhua ; Mao, Hongju ; Jin, Qinghui ; Wu, Lei ; Zhao, Hui ; Zhao, Jianlong</creatorcontrib><description>We report here a quantum dots-DNA (QDs-DNA) nanosensor based on fluorescence resonance energy transfer (FRET) for the detection of the target DNA and single mismatch in hepatitis B virus (HBV) gene. The proposed one-pot DNA detection method is simple, rapid and efficient due to the elimination of the washing and separation steps. In this study, the water-soluble CdSe/ZnS QDs were prepared by replacing the trioctylphosphine oxide (TOPO) on the surface of QDs with 3-mercaptopropionic acid (MPA). Subsequently, oligonucleotides were attached to the QDs surface to form functional QDs-DNA conjugates. Along with the addition of DNA targets and Cy5-modified signal DNAs into the QDs-DNA conjugates, sandwiched hybrids were formed. The resulting assembly brings the Cy5 fluorophore, the acceptor, and the QDs, the donor, into proximity, leading to fluorescence emission from the acceptor by means of FRET on illumination of the donor. In order to efficiently detect single-base mutants in HBV gene, oligonucleotide ligation assay was employed. If there existed a single-base mismatch, which could be recognized by the ligase, the detection probe was not ligated and no Cy5 emission was produced due to the lack of FRET. The feasibility of the proposed method was also demonstrated in the detection of synthetic 30-mer oliginucleotide targets derived from the HBV with a sensitivity of 4.0
nM by using a multilabel counter. The method enables a simple and efficient detection that could be potentially used for high throughput and multiplex detections of target DNA and the mutants.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2010.01.007</identifier><identifier>PMID: 20138498</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; DNA ; DNA Mutational Analysis - instrumentation ; DNA, Viral - analysis ; DNA, Viral - genetics ; Equipment Design ; Equipment Failure Analysis ; Fluorescence resonance energy transfer (FRET) ; Fluorescence Resonance Energy Transfer - instrumentation ; Fundamental and applied biological sciences. Psychology ; Hepatitis B virus (HBV) ; Hepatitis B virus - genetics ; Mutation ; Nanotechnology - instrumentation ; Oligonucleotide Array Sequence Analysis - instrumentation ; Quantum Dots ; Quantum dots (QDs) ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Biosensors & bioelectronics, 2010-04, Vol.25 (8), p.1934-1940</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-af37c6bc0478ed7d84c791b8ff5d6384a387a28dbdf7ebc15890e8c4092209163</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2010.01.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22566238$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20138498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xiang</creatorcontrib><creatorcontrib>Lou, Xinhui</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Guo, Qingchuan</creatorcontrib><creatorcontrib>Fang, Zheng</creatorcontrib><creatorcontrib>Zhong, Xinhua</creatorcontrib><creatorcontrib>Mao, Hongju</creatorcontrib><creatorcontrib>Jin, Qinghui</creatorcontrib><creatorcontrib>Wu, Lei</creatorcontrib><creatorcontrib>Zhao, Hui</creatorcontrib><creatorcontrib>Zhao, Jianlong</creatorcontrib><title>QDs-DNA nanosensor for the detection of hepatitis B virus DNA and the single-base mutants</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>We report here a quantum dots-DNA (QDs-DNA) nanosensor based on fluorescence resonance energy transfer (FRET) for the detection of the target DNA and single mismatch in hepatitis B virus (HBV) gene. The proposed one-pot DNA detection method is simple, rapid and efficient due to the elimination of the washing and separation steps. In this study, the water-soluble CdSe/ZnS QDs were prepared by replacing the trioctylphosphine oxide (TOPO) on the surface of QDs with 3-mercaptopropionic acid (MPA). Subsequently, oligonucleotides were attached to the QDs surface to form functional QDs-DNA conjugates. Along with the addition of DNA targets and Cy5-modified signal DNAs into the QDs-DNA conjugates, sandwiched hybrids were formed. The resulting assembly brings the Cy5 fluorophore, the acceptor, and the QDs, the donor, into proximity, leading to fluorescence emission from the acceptor by means of FRET on illumination of the donor. In order to efficiently detect single-base mutants in HBV gene, oligonucleotide ligation assay was employed. If there existed a single-base mismatch, which could be recognized by the ligase, the detection probe was not ligated and no Cy5 emission was produced due to the lack of FRET. The feasibility of the proposed method was also demonstrated in the detection of synthetic 30-mer oliginucleotide targets derived from the HBV with a sensitivity of 4.0
nM by using a multilabel counter. The method enables a simple and efficient detection that could be potentially used for high throughput and multiplex detections of target DNA and the mutants.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>DNA</subject><subject>DNA Mutational Analysis - instrumentation</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fluorescence resonance energy transfer (FRET)</subject><subject>Fluorescence Resonance Energy Transfer - instrumentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hepatitis B virus (HBV)</subject><subject>Hepatitis B virus - genetics</subject><subject>Mutation</subject><subject>Nanotechnology - instrumentation</subject><subject>Oligonucleotide Array Sequence Analysis - instrumentation</subject><subject>Quantum Dots</subject><subject>Quantum dots (QDs)</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo7uzqH_AguYinHvPR3UmDl3V3_YBFEfTgKaSTipuhJz2m0gv-e9POqDcPRUHxvEXxFCHPONtyxvtXu-0YZ9wKVgeMbxlTD8iGayWbVsjuIdmwoeubru_lGTlH3LFK8IE9Jmc1InU76A359vkam-uPlzTZNCMknDMNtcodUA8FXIlzonOgd3CwJZaI9A29j3lBuqZs8r9RjOn7BM1oEeh-KTYVfEIeBTshPD31C_L17c2Xq_fN7ad3H64ubxvXdrw0Nkjl-tGxVmnwyuvWqYGPOoTO9_VIK7WyQvvRBwWj450eGGjXskEINvBeXpCXx72HPP9YAIvZR3QwTTbBvKBRUgrJWd9WUhxJl2fEDMEcctzb_NNwZlajZmdWo2Y1ahg31VcNPT-tX8Y9-L-RPwor8OIEWHR2CtkmF_EfJ-oDhFy510cOqoz7CNmgi5Ac-JirZuPn-L87fgHUSJM3</recordid><startdate>20100415</startdate><enddate>20100415</enddate><creator>Wang, Xiang</creator><creator>Lou, Xinhui</creator><creator>Wang, Yi</creator><creator>Guo, Qingchuan</creator><creator>Fang, Zheng</creator><creator>Zhong, Xinhua</creator><creator>Mao, Hongju</creator><creator>Jin, Qinghui</creator><creator>Wu, Lei</creator><creator>Zhao, Hui</creator><creator>Zhao, Jianlong</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100415</creationdate><title>QDs-DNA nanosensor for the detection of hepatitis B virus DNA and the single-base mutants</title><author>Wang, Xiang ; Lou, Xinhui ; Wang, Yi ; Guo, Qingchuan ; Fang, Zheng ; Zhong, Xinhua ; Mao, Hongju ; Jin, Qinghui ; Wu, Lei ; Zhao, Hui ; Zhao, Jianlong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-af37c6bc0478ed7d84c791b8ff5d6384a387a28dbdf7ebc15890e8c4092209163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>DNA</topic><topic>DNA Mutational Analysis - instrumentation</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - genetics</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fluorescence resonance energy transfer (FRET)</topic><topic>Fluorescence Resonance Energy Transfer - instrumentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hepatitis B virus (HBV)</topic><topic>Hepatitis B virus - genetics</topic><topic>Mutation</topic><topic>Nanotechnology - instrumentation</topic><topic>Oligonucleotide Array Sequence Analysis - instrumentation</topic><topic>Quantum Dots</topic><topic>Quantum dots (QDs)</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiang</creatorcontrib><creatorcontrib>Lou, Xinhui</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Guo, Qingchuan</creatorcontrib><creatorcontrib>Fang, Zheng</creatorcontrib><creatorcontrib>Zhong, Xinhua</creatorcontrib><creatorcontrib>Mao, Hongju</creatorcontrib><creatorcontrib>Jin, Qinghui</creatorcontrib><creatorcontrib>Wu, Lei</creatorcontrib><creatorcontrib>Zhao, Hui</creatorcontrib><creatorcontrib>Zhao, Jianlong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiang</au><au>Lou, Xinhui</au><au>Wang, Yi</au><au>Guo, Qingchuan</au><au>Fang, Zheng</au><au>Zhong, Xinhua</au><au>Mao, Hongju</au><au>Jin, Qinghui</au><au>Wu, Lei</au><au>Zhao, Hui</au><au>Zhao, Jianlong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>QDs-DNA nanosensor for the detection of hepatitis B virus DNA and the single-base mutants</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2010-04-15</date><risdate>2010</risdate><volume>25</volume><issue>8</issue><spage>1934</spage><epage>1940</epage><pages>1934-1940</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>We report here a quantum dots-DNA (QDs-DNA) nanosensor based on fluorescence resonance energy transfer (FRET) for the detection of the target DNA and single mismatch in hepatitis B virus (HBV) gene. The proposed one-pot DNA detection method is simple, rapid and efficient due to the elimination of the washing and separation steps. In this study, the water-soluble CdSe/ZnS QDs were prepared by replacing the trioctylphosphine oxide (TOPO) on the surface of QDs with 3-mercaptopropionic acid (MPA). Subsequently, oligonucleotides were attached to the QDs surface to form functional QDs-DNA conjugates. Along with the addition of DNA targets and Cy5-modified signal DNAs into the QDs-DNA conjugates, sandwiched hybrids were formed. The resulting assembly brings the Cy5 fluorophore, the acceptor, and the QDs, the donor, into proximity, leading to fluorescence emission from the acceptor by means of FRET on illumination of the donor. In order to efficiently detect single-base mutants in HBV gene, oligonucleotide ligation assay was employed. If there existed a single-base mismatch, which could be recognized by the ligase, the detection probe was not ligated and no Cy5 emission was produced due to the lack of FRET. The feasibility of the proposed method was also demonstrated in the detection of synthetic 30-mer oliginucleotide targets derived from the HBV with a sensitivity of 4.0
nM by using a multilabel counter. The method enables a simple and efficient detection that could be potentially used for high throughput and multiplex detections of target DNA and the mutants.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>20138498</pmid><doi>10.1016/j.bios.2010.01.007</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0956-5663 |
ispartof | Biosensors & bioelectronics, 2010-04, Vol.25 (8), p.1934-1940 |
issn | 0956-5663 1873-4235 |
language | eng |
recordid | cdi_proquest_miscellaneous_733231064 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology DNA DNA Mutational Analysis - instrumentation DNA, Viral - analysis DNA, Viral - genetics Equipment Design Equipment Failure Analysis Fluorescence resonance energy transfer (FRET) Fluorescence Resonance Energy Transfer - instrumentation Fundamental and applied biological sciences. Psychology Hepatitis B virus (HBV) Hepatitis B virus - genetics Mutation Nanotechnology - instrumentation Oligonucleotide Array Sequence Analysis - instrumentation Quantum Dots Quantum dots (QDs) Reproducibility of Results Sensitivity and Specificity |
title | QDs-DNA nanosensor for the detection of hepatitis B virus DNA and the single-base mutants |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T12%3A43%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=QDs-DNA%20nanosensor%20for%20the%20detection%20of%20hepatitis%20B%20virus%20DNA%20and%20the%20single-base%20mutants&rft.jtitle=Biosensors%20&%20bioelectronics&rft.au=Wang,%20Xiang&rft.date=2010-04-15&rft.volume=25&rft.issue=8&rft.spage=1934&rft.epage=1940&rft.pages=1934-1940&rft.issn=0956-5663&rft.eissn=1873-4235&rft_id=info:doi/10.1016/j.bios.2010.01.007&rft_dat=%3Cproquest_cross%3E733231064%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733231064&rft_id=info:pmid/20138498&rft_els_id=S0956566310000199&rfr_iscdi=true |